全文获取类型
收费全文 | 2815篇 |
免费 | 204篇 |
国内免费 | 12篇 |
专业分类
耳鼻咽喉 | 17篇 |
儿科学 | 117篇 |
妇产科学 | 53篇 |
基础医学 | 284篇 |
口腔科学 | 76篇 |
临床医学 | 303篇 |
内科学 | 618篇 |
皮肤病学 | 58篇 |
神经病学 | 255篇 |
特种医学 | 288篇 |
外科学 | 454篇 |
综合类 | 31篇 |
一般理论 | 1篇 |
预防医学 | 175篇 |
眼科学 | 21篇 |
药学 | 161篇 |
中国医学 | 12篇 |
肿瘤学 | 107篇 |
出版年
2023年 | 16篇 |
2022年 | 23篇 |
2021年 | 61篇 |
2020年 | 41篇 |
2019年 | 74篇 |
2018年 | 100篇 |
2017年 | 49篇 |
2016年 | 70篇 |
2015年 | 89篇 |
2014年 | 97篇 |
2013年 | 101篇 |
2012年 | 158篇 |
2011年 | 148篇 |
2010年 | 105篇 |
2009年 | 110篇 |
2008年 | 135篇 |
2007年 | 110篇 |
2006年 | 94篇 |
2005年 | 100篇 |
2004年 | 93篇 |
2003年 | 105篇 |
2002年 | 66篇 |
2001年 | 74篇 |
2000年 | 71篇 |
1999年 | 58篇 |
1998年 | 60篇 |
1997年 | 60篇 |
1996年 | 74篇 |
1995年 | 56篇 |
1994年 | 49篇 |
1993年 | 51篇 |
1992年 | 38篇 |
1991年 | 23篇 |
1990年 | 32篇 |
1989年 | 54篇 |
1988年 | 40篇 |
1987年 | 42篇 |
1986年 | 46篇 |
1985年 | 39篇 |
1984年 | 29篇 |
1983年 | 21篇 |
1982年 | 26篇 |
1981年 | 18篇 |
1980年 | 12篇 |
1979年 | 16篇 |
1978年 | 10篇 |
1977年 | 17篇 |
1976年 | 17篇 |
1975年 | 19篇 |
1974年 | 6篇 |
排序方式: 共有3031条查询结果,搜索用时 15 毫秒
61.
A. Florentino A. Castro J.A. Fuentes 《Clinical and experimental hypertension (New York, N.Y. : 1993)》2013,35(5):873-885
ABSTRACTHigh systolic blood pressure (BP) was induced in young Wistar rats by daily exposure for 30 mins to environmental stimuli consisting of intense (100 dB) sound of 7500 cps and flashing light (0.3 cps). Maximal BP enhancement was obtained after the application of both these stimuli for 3 consecutive days. Such a hypertensive response was detected 24 hrs, but not 1 hr after the trial. High BP backed to normal 72 hrs after discontinuation of the audiovisual stimulation trials. Clinically effective antihypertensive agents (clonidine, prazosin, propranolol, practolol and metoprolol) as well as a new compound under investigation (cicletanine) were proved active in this model. Normotensive nonstressed rats run in parallel did not show any variation in their BP after administration of the same dose of the drugs assayed. This experimental model of hypertension may be a useful tool not only to test new antihypertensive agents but also to study the intriguing question of the role of stress in the development and maintenance of essential hypertension. 相似文献
62.
63.
Carmen María Martín-Navarro Jacob Lorenzo-Morales Rubén P. Machin Atteneri López-Arencibia José Manuel García-Castellano Isabel de Fuentes Brendan Loftus Sutherland K. Maciver Basilio Valladares José E. Pi?ero 《Antimicrobial agents and chemotherapy》2013,57(1):375-381
Acanthamoeba is an opportunistic pathogen in humans, whose infections most commonly manifest as Acanthamoeba keratitis or, more rarely, granulomatous amoebic encephalitis. Although there are many therapeutic options for the treatment of Acanthamoeba, they are generally lengthy and/or have limited efficacy. Therefore, there is a requirement for the identification, validation, and development of novel therapeutic targets against these pathogens. Recently, RNA interference (RNAi) has been widely used for these validation purposes and has proven to be a powerful tool for Acanthamoeba therapeutics. Ergosterol is one of the major sterols in the membrane of Acanthamoeba. 3-Hydroxy-3-methylglutaryl–coenzyme A (HMG-CoA) reductase is an enzyme that catalyzes the conversion of HMG-CoA to mevalonate, one of the precursors for the production of cholesterol in humans and ergosterol in plants, fungi, and protozoa. Statins are compounds which inhibit this enzyme and so are promising as chemotherapeutics. In order to validate whether this enzyme could be an interesting therapeutic target in Acanthamoeba, small interfering RNAs (siRNAs) against HMG-CoA were developed and used to evaluate the effects induced by the inhibition of Acanthamoeba HMG-CoA. It was found that HMG-CoA is a potential drug target in these pathogenic free-living amoebae, and various statins were evaluated in vitro against three clinical strains of Acanthamoeba by using a colorimetric assay, showing important activities against the tested strains. We conclude that the targeting of HMG-CoA and Acanthamoeba treatment using statins is a novel powerful treatment option against Acanthamoeba species in human disease. 相似文献
64.
David G. Fuentes Rachel R. Ogden Ann Ryan-Haddad Aimee F. Strang 《American journal of pharmaceutical education》2015,79(3)
During our time in the 2013 Academic Leadership Fellows Program, we explored what it takes to achieve life balance through a framework presented in a Harvard Business Review article. In this Statement, we describe 5 different areas from the article that provide infrastructure for reflecting on how we have learned to approach life balance in academia. We also provide brief messages based on this reading and others to help academics’ pursuit of life balance. 相似文献
65.
Screening of candidate biomaterials for alveolar augmentation using a critical‐size rat calvaria defect model
下载免费PDF全文
![点击此处可从《Journal of clinical periodontology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
66.
67.
Rosa A González-Polo José M Bravo-San Pedro Rubén Gómez-Sánchez Elisa Pizarro-Estrella Mireia Niso-Santano José M Fuentes 《British journal of pharmacology》2013,168(1):60-62
Huntington''s disease (HD) is a neurodegenerative disorder caused by a mutation in the gene encoding the huntingtin protein. Although the precise mechanism by which neuronal degeneration occurs is still unclear, several elements are important to its development: (1) altered gene expression and protein synthesis, (2) mitochondrial damage and (3) improper regulation of the autophagy programme. In this issue of British Journal of Pharmacology, Galindo and co-workers provide the first evidence for a role of the mitochondrial permeability transition pore (mPTP) in mitochondrial fragmentation and autophagy activation. In a model of cell death induced by 3-nitropropionic acid (3-NP) in human neural cells, the authors describe clear functions for mPTP and Bax, but not the mitochondrial fusion/fission machinery, mitochondrial fragmentation and autophagy (mitophagy). This commentary summarises the significance of this relationship and suggests several points for future development. 相似文献
68.
69.
We have explored the polymorphism of the glycophorin system in the human erythrocyte membrane using the immunoblotting techniques and examining 52 individuals selected without prior bias as to their serologic state and ten documented serologic variants of M, N, S, s blood group system. Polyclonal antisera to alpha glycophorin and to alpha glycophorin CNBr carboxyl terminal fragment C (residues 82-131) and M and N specific monoclonal antibodies (MoAbs) were used. The first two reagents detect specific regions of the alpha glycophorin molecule and all electrophoretically resolved species of glycophorins immunologically related to alpha and delta glycophorins (delta glycophorin, [alpha-delta] hybrids and other glycophorins with an alteration in the carboxyl terminal segment); the M and N MoAbs identified the glycophorin species containing or lacking the M or N determinant in the amino terminal octapeptide structures. We find that immunoblotting confirmed in all cases the serologically determined phenotype; we also find that polymorphic forms of the glycophorin system are relatively infrequent; immunoblotting, independent from serologic testing, was capable of detecting five mutants, two most likely S-s-U-phenotypes; a new glycophorin species was detected in normal red cells with both antiglycophorin and antipeptide C sera, which is not evident with MoAbs; immunoblots of known glycophorin variants (En(a-), U-, Mg, Mi I, II, III, V, and Sta) confirmed but also extended our knowledge of the abnormal glycophorins involved; and the He+ and Wrb(-) cells showed normal patterns. 相似文献
70.
Cytogenetic and histologic correlations in malignant lymphoma 总被引:9,自引:0,他引:9
Koduru PR; Filippa DA; Richardson ME; Jhanwar SC; Chaganti SR; Koziner B; Clarkson BD; Lieberman PH; Chaganti RS 《Blood》1987,69(1):97-102
Although a number of studies have indicated correlations between histologic subtypes of tumors and certain nonrandom chromosome changes, cytogenetic studies of lymphoma are in an early stage compared to those of leukemia. No comprehensive analysis of available data has so far been attempted in the literature either. Here we present an analysis of chromosome changes and their correlation with subtypes of lymphoma studied by conventional histology and cell surface markers, as observed in two sets of data: a group of 65 karyotypically abnormal tumors sequentially ascertained and studied by us during the period January 1, 1984 to April 30, 1985, and a larger data set derived by combining our data with those from two published series from the University of Minnesota that are comparable to our data. These combined data, which comprise the largest data set on the cytogenetics of lymphomas assembled so far, enabled a comprehensive analysis of correlation between chromosome change and tumor histology and the patterns of chromosome instability in these tumors. We found several significant associations, some previously described and others now recognized, between nonrandom chromosome gains, breaks, translocations, and deletions and histologic subtypes of tumors that characterize lymphomas. The data indicate that finding of chromosome breaks at certain sites (eg, 8q24, 14q32, 18q21) is of diagnostic value in dealing with cases of unusual lymphoma. Furthermore, nonrandom chromosome breakage exhibited three distinct patterns that reflected three levels of etiologically relevant genetic change. 相似文献