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101.
目的 研究原发性肝癌合并糖尿病的介入治疗方法。方法 临床明确诊断的 3 4例肝癌合并糖尿病患者在介入治疗前后通过口服降糖药或注射胰岛素将血糖、尿糖控制在安全范围内 ,并对隐性感染或感染源进行治疗 ,然后再行肝动脉化疗灌注栓塞术。结果  3 4例患者介入治疗前血糖均降到 9.0mmol/L以下 ,尿糖控制在 -~ ++,术后均未发生严重并发症 ,仅有 2例出现慢性胆囊炎急性发作 ,经抗炎、利胆治疗后安全出院。结论 肝癌合并糖尿病患者在血糖、尿糖降到一定的范围内 ,消除潜在的感染 ,行肝动脉化疗栓塞治疗是安全可行的  相似文献   
102.
反相高压液相色谱法测定血清中部分氨基酸   总被引:8,自引:0,他引:8  
目的:建立测定血清中精氨酸含量的方法。方法:反相高压液相色谱法。HypersilBDS柱,邻苯二甲醛柱前衍生化、萤光检测法检测。结果:精氨酸线性范围为383μmol/L~4789μmol/L,8种氨基酸的最低检测限为2μmol/L,日内变异系数为275%~1193%,日间变异系数为705%~1239%,5种氨基酸的回收率为9633%~10078%。正常人血清精氨酸水平为9277±1790μmol/L,糖尿病患者血清精氨酸水平则为7861±3303μmol/L(P>005)。结论:本方法简单易行,方法学实验结果满意,适用于临床测定相关氨基酸的需要  相似文献   
103.
检测25例脑肿瘤中抑制癌基因P53突变情况。突用复合PCR,聚合酶链反应-=单链构象多态性分析法,统计学处理采用X^2检验。结果:P53基因总的突变率为44.0%,其中第5外显子突变率为28.0%,第6外显子突变率12.0%,第7外显子突变率为12.0%,第8外显子突变率16.0%;病理分级Ⅱ级及其以上突变为72.7%,  相似文献   
104.
石棉相关肿瘤p53基因突变的免疫组化及PCR—SSCP研究   总被引:3,自引:0,他引:3  
为了分析石棉相关肿瘤p53基因的突变特点,对石蜡包埋的石棉相关肿瘤p53基因突变体蛋白的表达进行了免疫组化观察,提取染色体DNA,对p53基因的第5、7、8外显子进行PCR-SSCP及测序分析。免疫组化观察发现:在分析的10例病例中有5例阳性。PCR-SSCP分析发现7例(8处)发生突变,其中4处集中在第8外显子上。5例腺癌中有4例发生p53基因突变。测序发现热点区突变。提示:石棉相关肿瘤p53基因突变率高。  相似文献   
105.
106.
采用动脉血氧饱和度仪对52例新生儿肺炎患儿,在雾化吸入治疗过程中的动脉血氧饱和度(SaO2)进行了监测,对比了吸氧与否对SaO2改变的影响。结查表明:与基础值比较,雾化吸入时患儿的SaO2显著下降(P<0.01),而雾化吸入同时吸氧者SaO2无明显变化(P>0.05),但两者比较具有显著性差异(P<0.01)。结果提示雾化吸入可使新生儿肺炎患儿SaO2下降;同时吸氧,对防止SaO2的下降有一定作用。  相似文献   
107.
采用大剂量甲基强的松龙治疗脱髓鞘脑病患者15例。在治疗过程中,积极预防应激性溃疡,加强呼吸道及人工冬眠护理。经5次治疗,14例症状明显改善。  相似文献   
108.
Fu SL  Ma ZW  Yin L  Iannotti C  Lu PH  Xu XM 《Neuroscience》2005,135(3):851-862
To determine whether neural precursor cells have region-specific growth properties, we compared the proliferation, mitogenicity, and differentiation of these cells isolated from the embryonic day 16 rat forebrain and spinal cord. Neural precursor cells isolated from both regions were cultured in growth medium supplemented with epidermal growth factor, basic fibroblast growth factor, or epidermal growth factor+basic fibroblast growth factor. Under all three conditions, both neural precursor cell populations proliferated for multiple passages. While spinal cord-derived neural precursor cells proliferated moderately faster in epidermal growth factor-enriched growth medium, brain-derived cells proliferated much faster in basic fibroblast growth factor-enriched growth medium. When exposed to both epidermal growth factor and basic fibroblast growth factor, the two neural precursor cell populations expanded and proliferated more rapidly than when exposed to a single factor, with brain-derived neural precursor cells expanding significantly faster than spinal cord-derived ones (P<0.0001). Differentiation studies showed that both neural precursor cell populations were multi-potent giving rise to neurons, astrocytes, and oligodendrocytes. However, neuronal differentiation from brain-derived neural precursor cells was greater than spinal cord-derived ones (11.95+/-5.00% vs 1.92+/-1.13%; passage 2). Further, the two neural precursor cell populations differentiated into a similar percentage of oligodendrocytes (brain: 8.66+/-5.85%; spinal cord: 7.69+/-3.91%; passage 2). Immunofluorescence and Western blot studies showed that neural precursor cells derived from both regions expressed receptors for basic fibroblast growth factor and epidermal growth factor. However, brain-derived neural precursor cells expressed higher levels of the two receptors than spinal cord-derived ones in growth medium containing epidermal growth factor+basic fibroblast growth factor. Thus, our results showed that neural precursor cells isolated from the two regions of the CNS have distinct properties and growth requirements. Identifying phenotypic differences between these neural precursor cell populations and their growth requirements should provide new insights into the development of cell therapies for region-specific neurological degenerative diseases.  相似文献   
109.
On December 31, 2001, 2486 patients with terminal renal failure received dialysis treatment in Croatia. Only one third of the patients are registered on the national waiting list for cadaveric kidney transplant. In most of the others, transplantation is impossible because of comorbidity. This is mainly due to the steadily growing age of the dialytic population and therefore a higher incidence of cardiovascular disease and diabetes. Still, evaluation of the potential recipients of cadaveric kidney transplant, registered on the waiting list, often reveals contraindications for transplantation. The aim of this study was to determine the incidence and type of contraindications in transplant candidates, found during immediate preoperative evaluation. Analysis of these data should help in determining how contraindications can be early detected and prevented. Before registering onto the national waiting list transplant candidates need to be thoroughly investigated including detailed history, physical examination, routine diagnostic procedures and additional examinations, if needed, to exclude or evaluate the possibly existing contraindications for transplantation. During the period from January 1997 until June 2002, 145 potential recipients from the national waiting list were referred to the Rijeka University Hospital Center and evaluated for kidney transplantation. Eighty-eight patients underwent transplantation. Preoperative evaluation revealed contraindications for transplantation in 52 (35.9%) candidates. Twenty-two (15.2%) patients had a positive cross-match with donor lymphocytes, 6 (4.1%) patients refused transplantation, and in 24 (16.6%) patients serious comorbidity was the reason for not being accepted for transplantation and for their withdrawal from the national waiting list. Comorbidity was mainly due to cardiovascular disease (12 patients--8.3%) and infection (8 patients--5.5%). These data show a high incidence of contraindications found during the immediate preoperative evaluation of potential kidney recipients. It was the case in more than one third of patients. During the evaluation of potential candidates for kidney transplantation special attention should be addressed to the presence of cardiovascular morbidity and infection. Peripheral vascular occlusive disease, cardiac status and/or cerebrovascular disease should be evaluated. Measures used to treat or reduce the development of complications include an optimal control of blood pressure, serum phosphate, hyperparathyroidism, dyslipidemia, and renal anemia. The sites of infection must be treated and eradicated, because immunosuppressive treatment is a threat to the transplant recipient's life. The second most common cause of refusal of potential candidates was a positive cross-match with donor lymphocytes. Sensitization to human leukocyte antigens can be prevented by the avoiding of blood transfusions and use of erythopoietin in treating renal anemia. To minimize the morbidity and mortality, the potential kidney recipients should undergo rigorous selection and thorough evaluation before including them into the waiting list for kidney transplantation. Afterwards, regular examinations are obligatory to reveal contraindications, proceed to medical interventions and treat concomitant diseases in time, which can influence the patient's survival. In case that contraindications for transplantation arise, the patient must be temporarily or definitely removed from the waiting list.  相似文献   
110.
利用指数衰减原理来抑制 x射线图像中小密度组织对骨骼边缘的影响 ,利用该方法很容易使用直方图原理提取出骨骼的边缘和内部结构变化的二值图 ,并有效地抑制了背景对骨骼边缘的影响 ,结合 canny算子的结果 ,可以有效地提取人体复杂结构中骨骼的边缘。  相似文献   
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