多学科护理团队协作模式在老年患者围手术期的应用

目的 探讨多学科护理团队协作模式在老年围手术期患者中的应用效果.方法 选取笔者所在科室2018年1—12月就诊并行手术治疗的65岁以上老年患者50例为对照组,选取2019年1—12月就诊行手术治疗的65岁以上老年患者50例为观察组.对照组给予老年科常规围手术期护理,观察组在对照组基础上给予多学科护理团队协作模式进行干预...     

Golgi scaffold protein PAQR3 as a candidate suppressor of gastric cardia adenocarcinoma via regulating TGF‐β/Smad pathway

ObjectivesTo investigate the function of PAQR3 in gastric cardia adenocarcinoma (GCA) and understand the possible mechanism of PAQR3 in regulating epithelial–mesenchymal transition (EMT).MethodsWe detected PAQR3 protein in 146 GCA tissues and paired normal adjacent tissues (PNTs) specimens using immunohistochemical analysis, and explored its clinical significance. The expression levels of PAQR3 protein in 20 GCA tissues, their paired PNTs, HGC27, SGC7901, and GES‐1 cells were analyzed by Western blot. Wild‐type PAQR3 was overexpressed in HGC27 cells. The effects of PAQR3 overexpression on the function of HGC27 cells and its underlying mechanisms were then analyzed through a series of cell and molecular biology experiments.ResultsPAQR3 was significantly down‐regulated in GCA tissues when compared with paired PNTs (p < 0.0001). The expression level of PAQR3 in GCA tissues was significantly negatively correlated with Helicobacter pylori infection (p = 0.000), venous invasion (p = 0.000), invasion depth (p = 0.000), lymph node metastasis (p = 0.022), tumor stage (p = 0.000), and patient survival (p = 0.009). Downregulation of PAQR3 was highly correlated with increased EMT signature and activated TGF‐β/Smad pathway in GCA tissues. Overexpression of PAQR3 in HGC27 cells negatively regulates its cellular functions, such as cell proliferation and migration, and suppresses EMT. Mechanistically, overexpression of PAQR3 significantly down‐regulates the protein expression levels of TGF‐1, p‐Smad2, and p‐Smad3 in HGC27 cells.ConclusionPAQR3 was significantly down‐regulated in GCA tissues, HGC27, and SGC7901 cells. PAQR3 significantly inhibits the proliferation, migration, and invasion of HGC27 cells. Mechanistically, PAQR3 can inhibit the EMT process in HGC27 cells by regulating TGF‐β/Smad signaling pathway.     

Evaluation and comparison of antioxidant abilities of five bioactive molecules with C–H and O–H bonds in thermodynamics and kinetics

In this work, the antioxidant abilities of NADH coenzyme analogue BNAH, F420 reduction prototype analogue F420H, vitamin C analogue iAscH⁻, caffeic acid, and (+)-catechin in acetonitrile in chemical reactions were studied and discussed. Three physical parameters of the antioxidant XH, homolytic bond dissociation free energy ΔG^°(XH), self-exchange HAT reaction activation free energy ΔG^≠_XH/X, and thermo-kinetic parameter ΔG^≠°(XH), were used to evaluate the antioxidant ability of XH in thermodynamics, kinetics, and thermo-kinetics. By comparing ΔG^°(XH), ΔG^≠_XH/X and ΔG^≠°(XH) of these five bioactive antioxidants to release hydrogen atoms, it is easy to find that iAscH⁻ is the best hydrogen atom donor both thermodynamically and kinetically among these antioxidants. Caffeic acid is the worst hydrogen atom donor thermodynamically, and F420H is the worst hydrogen atom donor kinetically. In addition, the thermodynamic hydride donating abilities of BNAH, F420H, and iAscH⁻ were also discussed, and the order of thermodynamic hydride donating abilities was BNAH > F420H > iAscH⁻. Four HAT reactions BNAH/DPPH^˙, (+)-catechin/DPPH^˙, F420H/DPPH^˙, and caffeic acid/DPPH^˙ in acetonitrile at 298 K were studied by the stopped-flow method. The actual order of H-donating abilities of these four antioxidants in the HAT reactions is consistent with the order predicted by thermo-kinetic parameters. It is feasible to predict accurately the antioxidant abilities of antioxidants using thermo-kinetic parameters.

In this work, the antioxidant abilities of NADH coenzyme analogue BNAH, F420 reduction prototype analogue F420H, vitamin C analogue iAscH⁻, caffeic acid, and (+)-catechin in acetonitrile in chemical reactions were studied and discussed.     

IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer

Bone is a common site of metastasis in lung cancer, but the regulatory mechanism remains incompletely understood. Osteoclasts are known to play crucial roles in osteolytic bone metastasis by digesting bone matrix and indirectly enhancing tumor colonization. In this study, we found that IL receptor 20 subunit β (IL-20RB) mediated a direct tumoral response to osteoclasts. Tumoral expression of IL-20RB was associated with bone metastasis of lung cancer, and functionally, IL-20RB promoted metastatic growth of lung cancer cells in bone. Mechanistically, tumor cells induced osteoclasts to secrete the IL-20RB ligand IL-19, and IL-19 stimulated IL-20RB–expressing tumor cells to activate downstream JAK1/STAT3 signaling, leading to enhanced proliferation of tumor cells in bone. Importantly, blocking IL-20RB with a neutralizing antibody significantly suppressed bone metastasis of lung cancer. Overall, our data revealed a direct protumor role of osteoclastic niche in bone metastasis and supported IL-20RB–targeting approaches for metastasis treatment.     

宜昌夷陵地区儿童肥胖合并哮喘调查及影响因素

目的 探究宜昌市夷陵地区儿童肥胖合并哮喘患儿调查及影响因素.方法 采用分层随机整群抽样方法选取2018-2019年夷陵地区肥胖合并哮喘患儿379例,同期210例体检健康儿童作为对照组.通过问卷调查方式获取患儿一般资料,并进行单因素及多因素logistic分析.结果 肥胖伴哮喘患儿男性、居住在城市、存在哮喘家族史、二手烟...     

MCU在高糖诱导心肌H9c2细胞凋亡中的作用机制

目的:探讨线粒体钙离子单向转运体(mitochondrial calcium uniporter, MCU)在高糖(high glucose, HG)诱导心肌H9c2细胞凋亡中的作用机制。方法:将心肌H9c2细胞随机分为3组:对照(control)组,5.5 mmol/L葡萄糖处理细胞;HG组,25 mmol/L葡萄糖处理细胞;精胺(spermine,Sp)干预(HG+Sp)组,25 mmol/L葡萄糖和5μmol/L Sp共同处理细胞。Western blot检测H9c2细胞MCU、caspase-9和caspase-3蛋白的表达;RT-qPCR检测H9c2细胞MCU的mRNA水平;Rhod-2 AM探针检测线粒体内Ca²⁺的荧光强度;吸光度法检测丙酮酸脱氢酶(pyruvate dehydrogenase, PDH)的活性;萤火虫萤光素酶检测细胞裂解液ATP的浓度;JC-1染色法检测线粒体膜电位(mitochondrial membrane potential,Δψ_m);MitoSOX^TM染色法检测线粒体活性氧簇(re...     

Highly selective NH3 gas sensor based on Co(OH)2/Ti3C2Tx nanocomposites operating at room temperature

Ammonia (NH₃) is a common air pollutant and is a biomarker for kidney disease. Therefore, the preparation of ammonia gas sensors with high sensitivity, good selectivity and low operating temperature is of great importance for health protection. Using the in situ electrostatic self-assembly approach, a chemoresistive gas sensor based on Co(OH)₂/Ti₃C₂T_x hybrid material was created in this study. The prepared samples were characterized by XRD, XPS, TEM, BET and other testing methods for structure, surface topography and elements. These samples were fabricated into sensors, and the gas sensing properties of the materials were investigated under different test conditions. The results show that the gas response value of the C/M-2 sensor is up to about 14.7%/100 ppm, which is three times the response value of the sensor made of pure MXene to NH₃. In addition, the Co(OH)₂/Ti₃C₂T_x hybrid sensors exhibit excellent repeatability, high sensitivity under low concentration (less than 5 ppm), fast response/recovery time (29 s/49 s) and long-time stability, which indicates their promising utility in the IoT field.

The prepared Co(OH)₂@Ti₃C₂T_x composite shows good gas sensing performance for ammonia at room temperature, making it a promising candidate for a practical detector for NH₃.     

球面对称设计结合遗传神经网络优选黄芪-红花药对水提工艺

目的 采用球面对称设计及遗传神经网络优选黄芪-红花药对的水提工艺.方法 将黄芪-红花药对水提液中8种有效成分(羟基红花黄色素A、紫丁香苷、毛蕊异黄酮苷、脱水红花黄色素B、山柰酚-3-O-芸香糖苷、芒柄花苷、毛蕊异黄酮、黄芪甲苷)的含量进行赋权,以计算得到的综合得分作为水提工艺的评价指标.在单因素实验基础上,选择液料比、...     

益气活血泄浊法治疗慢性肾衰竭的临床疗效观察

目的:探究益气活血泄浊法治疗浊毒内蕴、肾气衰惫型慢性肾衰竭患者的效果。方法:选取2018年10月至2019年4月辽宁中医药大学附属医院收治的慢性肾衰竭患者100例作为研究对象,按照随机数字表法随机分为对照组和观察组,每组50例。对照组患者进行利水消肿、纠正电解质失衡、控制心率血压、低盐低脂饮食等常规西医治疗。观察组患者在对照组患者的治疗基础上给予益气泄浊汤口服。治疗周期为15 d。观察2组患者治疗前后中医证候积分、血肌酐(SCr)、转化生长因子(TGF-β)、Ⅳ-C、肝细胞生长因子(HGF)及临床有效率情况。结果:治疗后,2组的中医证候主证评分、SCr、TGF-β、HGF、Ⅳ-C含量较治疗前均有所改善,且观察组明显优于对照组,差异有统计学意义(P<0.05)。治疗后对照组临床总有效率为70%,观察组临床总有效率为88%,差异有统计学意义(P<0.05)。结论:益气泄浊汤治疗浊毒内蕴、肾气衰惫型慢性肾衰竭可有效抑制肾纤维化形成,提高肾小球滤过率,改善临床症状,提高临床疗效。     

赤参壮骨方配合经皮微创内固定术治疗四肢骨折的临床观察

目的 分析赤参壮骨方配合经皮微创内固定术在四肢骨折治疗中的应用价值.方法 选取2019年1月—2020年1月期间于华北石油管理局总医院收治的94例四肢骨折患者,随机分为对照组47例和治疗组47例.两组均行经皮微创内固定术,术后给予常规抗感染处理,治疗组在此基础上配合赤参壮骨方治疗,连续服用8周.比较两组手术及术后基本情...