The impact of human herpesvirus 6B reactivation on early complications following allogeneic hematopoietic stem cell transplantation.

Although human herpesvirus 6 (HHV-6) has been considered an important opportunistic and potentially fatal pathogen for allogeneic hematopoietic stem cell transplantation (HSCT), the clinical significance of HHV-6 reactivation remains controversial. In this study, we monitored HHV-6 DNAemia in 72 consecutive allogeneic HSCT recipients by real-time quantitative polymerase chain reaction. A total of 680 peripheral blood specimens were collected from the recipients before HSCT or at weekly intervals after HSCT. As the predominant variant, HHV-6B was detectable at least once in 47.2% (34/72) of HSCT recipients on the median day 21 (range, 7-84 days); HHV-6A reactivation occurred in only 1 recipient (1.4%). Detectable HHV-6B reactivation was associated with increased probability of skin rash by day 30 after HSCT (hazard ratio [HR], 3.68; 95% confidence interval [CI], 1.24-10.92; P = .019), cytomegalovirus (CMV) antigenemia (HR, 2.35; 95%CI, 1.32-4.19; P = .004), and hemorrhagic cystitis (HC) (HR, 2.59; 95%CI, 0.96-6.98; P = .061) by day 100 after HSCT. Neutrophil and platelet engraftment, mortality for 100 days after HSCT were not affected by HHV-6B reactivation. In conclusion, HHV-6 reactivation is a common event, and this study demonstrates a correlation between HHV-6B infection and CMV reactivation, early rash, and possibly increased incidence of HC after transplantation.     

Design and Simulation of Active Biochip System

To solve the problems existing in passive biochip systems, we designed a novel active biochip system. This system introduces negative pressure and controlling devices to adjust the antigen-antibody reaction on the nitrocellulose membrane. Computational simulation demonstrated that this system is a rapid, stable, robust and practical system that may enhance the efficiency of antigen-antibody reactions and improve the repeatability and accuracy of biochip analysis.     

益肾化湿颗粒联合达格列净治疗糖尿病肾病脾肾气虚证临床研究

目的：观察益肾化湿颗粒联合达格列净治疗糖尿病肾病（DN） 脾肾气虚证的临床疗效及对血小板 反应蛋白1（TSP-1）、可溶性Axl 受体酪氨酸激酶（sAxl） 水平的影响。方法：采用随机数字表法将88 例DN 脾肾气虚证患者分为对照组与观察组各44 例。对照组给予单纯达格列净治疗,观察组给予益肾化湿颗粒联合 达格列净治疗。比较2 组治疗前后中医证候积分、肾脏功能指标、血糖指标、TSP-1、sAxl 水平,评估2 组临 床疗效及不良反应发生情况。结果：治疗后,观察组总有效率93.18%,高于对照组77.27%（P＜0.05）。治疗 后,2 组中医证候积分、尿白蛋白/肌酐比值（UACR）、尿白蛋白排泄率（UAER）、空腹血糖（FBG）、餐后2 h 血糖（P2hBG）、糖化血红蛋白（HbA1c）、TSP-1、sAxl 水平均低于治疗前（P＜0.05）, 肾小球滤过 率（eGFR） 水平高于治疗前（P＜0.05）,且观察组治疗后UACR、UAER、TSP-1、sAxl 水平及血糖指标均低 于对照组（P＜0.05）,eGFR 水平高于对照组（P＜0.05）。2 组不良反应发生率比较,差异无统计学意义（P＞ 0.05）。结论：益肾化湿颗粒联合达格列净治疗DN 脾肾气虚证疗效显著,能有效改善患者肾脏功能,控制血 糖,降低TSP-1、sAxl 水平,安全性较高。     

An economic analysis of pancreas transplantation: costs, insurance coverage, and reimbursement

Since 1988 the demand for the pancreas transplantation has continued to increase. This has been accompanied by a growth in the number of centers offering the procedure, and an increase in the number of transplants performed. The National Cooperative Transplantation Study was undertaken to document the costs of all transplants, including pancreas transplantation. Data on transplantation procedure charges, from date of transplant to discharge, were obtained from 66.7% of all pancreas transplantation programs active in 1988. These programs accounted for 72% of all transplants performed that year. Valid sample survey data (no more than 25 transplants per center) were obtained for 133 randomly selected patients. This constituted 54% of all procedures done in the United States in 1988. Detailed data were also collected on sources of payment and amount reimbursed. Due to outlier data, we report statistical medians, rather than means, as our measure of central tendency. The median charge for a pancreas transplant with or without a kidney was $66917, with a hospital length of stay of 21 days, compared with a kidney transplant alone at $39625 and a hospital length of stay of 14 days. Total pancreas transplant charges fell between $45260 and $105375 for 50% of the cases studied. Half of the patients had a hospital length of stay between 16 and 33. Due to the small number of cases available for analysis, it was not meaningful to cross-classify the data according to various prognostic variables.(ABSTRACT TRUNCATED AT 250 WORDS)     

海水淹溺肺水肿兔血浆MDA和SOD的变化

目的 :观察海水淹溺肺水肿 (PE SWD)兔血浆中丙二醛 (MDA)和超氧化物歧化酶 (SOD)变化规律 ,探讨上述两项指标发生变化的原因和机制。方法 :用核黄素光照法和硫代巴比妥酸法 ,对PE SWD兔动脉血浆MDA和SOD进行定量检测和动态观察。结果 :灌海水后 30min ,MDA和SOD较灌海水前均显著升高 ,尔后两者均逐渐下降 ,但仍远远高于灌海水前。结论 :PE SWD发生发展过程中 ,MDA的损害性作用使肺泡表面活性物质表面特性发生改变 ,影响了正常的呼吸功能 ,并掩盖了SOD的保护性作用。     

The role of mtDNA deletion in the sensitivity to aminoglycoside antibiotic induced deafness]

OBJECTIVE: To establish an animal model of mitochondrial DNA (mtDNA) deletion and investigate the possible role of mtDNA deletion in aminoglycoside antibiotic induced deafness. METHODS: Thirty wistar rats (4 months) were randomly divided into group A and B. Doxorubicin (DOX) was subcutaneously injected at doses of 2 mg/kg twice per week for 3 months in group A and then kanamycin (KM) was intraperitoneally injected 500 mg/kg per day for 10 consecutive days. The treatments of group B were identical to group A, except normal saline was substituted for DOX. The thresholds of auditory brainstem response (ABR) were measured before and after the drug administrations. The inner ear membranous labyrinthine tissue was harvested and mtDNA was amplified to identify 4,834 bp deletion by PCR technique. RESULTS: The elevation of the mean ABR thresholds in group A(67.08 +/- 8.59) dB peSPL was significantly higher than that in group B (12.71 +/- 4.42) dB peSPL after KM administration (P < 0.001). In group A, 9 of the 15 rats demonstrated 4,834 bp mtDNA deletion. However, mtDNA 4,834 bp deletion was negative in group B animals. CONCLUSION: DOX can induce mtDNA deletion in the inner ear tissue of the rat. mtDNA deletion in the inner ear may play an important role in the hypersensitivity to aminoglycoside antibiotic ototoxicity.     

普萘洛尔(心得安)对灰鼠噪声性聋的影响

目的 研究噪声和β受体阻滞剂普萘洛尔（心得安）对灰鼠耳蜗复合动作电位和耳蜗血流的影响。方法 使用１／２倍频程窄带、噪声其中心频率１５００ＨＺ,强度１１５ｄＢＳＰＬ,持续刺激９０分钟。观察不同浓度普洛尔对灰鼠耳蜗复合动作电位和耳蜗充的影响结果     

多普勒组织显像评价高血压病心房内传导间期变化与房性心律失常的关系

目的　应用多普勒组织显像评价高血压病心房内传导间期变化与房性心律失常的关系 ;方法　利用超声多普勒组织显像法测量了 2 0例高血压病心房内传导间期与正常人对比分析 ;结果　高血压病心房内传导间期存在异常 ,心房内传导间期与左房内径大小无相关 (r =0 1,P >0 2 5 ) ,与左室舒张功能指标E A比值中度相关 (r=0 5 7,0 0 1

0 2 5 ) ;结论　高血压病房性心律失常与心房内传导异常及左室舒张功能有关 ,多普勒组织显像法是一种较好的测量高血压病心房内传导间期的方法     

EFFECT OF VL AND VH CONSENSUS SEQUENCE-SPECIFIC PRIMERS ON THE BINDING AND EXPRESSION OF A MINI- MOLECULE ANTIBODY DIRECTED TOWARDS HUMAN GASTRIC CANCER

Objective. To construct ScFv and Fab from murine anti-gastric cancer monoclonal antibody(mAb) 3H11.Methods. At first,3H11 ScFv and Fab were constructed with Ⅴ genes PCR amplified by degenerate primers for FR1 .The bacterial expressed 3H11 Ab fragments showed no antigen binding activity.Then,phage antibody library and random mutated library were constructed from 3H11 hybfidoma cells and panning selection was performed. Again the i-dentification of positive clone was failed. Finally the N-terminal sequences of Ⅴ regions were resumed to 3H11 original sequences by site-directed mutagenesis via PCR.Restdts. Binding activity to gastric cancer cells was detected only from N-terminal sequence corrected 3H11 ScFv and Fab, though the expression of the Ab fragments was not affected. Correction of either VL or VH N-terminal se-quences could partially resume the antigen binding activity. Conclusion. Sequence changes of Ⅴ region N terminal introduced by PCR may seriously affect antigen binding without affecting the expression of antibody.