Incorporation of magnesium ions into photo‐crosslinked alginate hydrogel enhanced cell adhesion ability

Photo‐crosslinked alginate hydrogel attracts wide interest in tissue engineering because of its excellent controllability and stability. However, its highly hydrophilic property makes cell adhesion difficult. Plenty of studies have confirmed that magnesium ions (Mg²⁺) can efficiently improve the attachment of osteoblasts. In this study, for the first time, we fabricated a durable, crosslinked, alginate hydrogel with a dual‐crosslinking network. Photo‐crosslinked alginate hydrogel was chosen as the basic backbone, and various amounts of Mg²⁺ were incorporated into the hydrogel through ionic crosslinking. The results showed that the physicochemical properties of the hydrogels, including surface structure, composition, swelling ratio, ion release and elastic modulus, could be well tuned by controlling the amount of Mg²⁺ incorporated. In addition, a certain amount of Mg²⁺ significantly improved the attachment and spread of osteoblasts on the hydrogels. These characteristics make Mg²⁺‐incorporated photo‐crosslinked alginate hydrogel a promising scaffold for bone tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.     

螺旋CT仿真支气管镜的临床应用价值

目的　研究螺旋ＣＴ仿真支气管镜（ＣＴＶＢ） 的临床应用价值。方法　对２５ 例患者进行ＣＴＶＢ检查,其中２２ 例经纤维支气管镜（ＦＯＢ） 检查,并将二者所见表现对照分析。结果　ＣＴＶＢ 获得的图像效果类似纤维支气管镜镜下所见的支气管腔内壁及腔内病变的表现。ＣＴＶＢ可以到达一般纤维支气管镜所不能到达的第Ｖ 级支气管并可以越过阻塞部位对远端的支气管进行观察。结论　螺旋ＣＴ 仿真支气管镜可以较真实地反映复杂气道的解剖关系及气道的形态改变,为临床提供了一种新的无创的诊断方法。     

盐酸替利定在Amberlite IRP-69上的静态离子交换特性

背景：离子交换树脂分子结构中含有可电离活化的基团,可以与离子性药物交换,使得药物进入其骨架而掩盖不良嗅味。目的：观察盐酸替利定在Amberlite IRP-69上的离子交换平衡特性、热力学及动力学特征。方法;采用静态离子交换法制备盐酸替利定树脂复合物,并进行离子交换平衡特性及动力学特征检测。结果与结论：在293,303,313K3个温度下及研究的浓度范围内,离子交换吸附等温方程、Freundlich方程和Langmuir方程都能够对盐酸替利定在Amberlite IRP-69上的离子交换行为进行很好的拟合。在290,298K下,其离子交换动力学特征基本符合一级动力学方程。盐酸替利定在Amberlite IRP-69上的离子交换热力学参数ΔH〉0及ΔS〉0,表明该离子交换反应为吸热反应,温度升高有利于反应正向进行,且该反应是熵增加的反应;离子交换自由能变ΔG〈0,说明该离子交换反应能够自发进行。初始质量浓度为5g/L的盐酸替利定在Amberlite IRP-69上的平衡交换吸附量为751.88g/L。提示在室温下,可利用一定浓度的盐酸替利定溶液,加入一定量的Amberlite IRP-69作为载体,持续搅拌2h制备药物树脂复合物,进而研制相关制剂。     

肿瘤相关科室护理人员自身健康状况及影响因素的调查

[目的]分析肿瘤相关科室护理人员自身健康状况的影响因素,为提高临床护理人员的自身健康状况,规范职业防护行为提供依据。[方法]采用江会设计的《护理人员抗肿瘤药物（化疗药物）职业防护状况个案调查表》对综合医院的418名肿瘤相关科室的护理人员进行调查。[结果]护理人员自身健康状况得分与医院的关系、职务、配药室设备、科室、配药室通风状况、接触抗肿瘤药物频繁程度不同的护理人员自身健康状况存在差异（P＆lt;0.05）,配药室设备配置对护理人员的自身健康状况有影响。[结论]管理部门应建立健全职业防护制度,规范职业防护行为,加大职业防护设施的配备。     

中医临床常见脉象分布情况调查

目的：调查临床常见脉象的分布情况,为脉象客观化研究提供参考。方法：调查和分析了全国23个省市、55家单位的156位高级职称中医师在临床诊脉过程中的体会。结果：（1）156位中医师临床体会过的脉象按出现频次由高到低排在前十位的依次为弦脉、数脉、沉脉、滑脉、迟脉、浮脉、细脉、结脉、代脉、虚脉;（2）110位中医师认为临床常见的脉象按出现频次由高到低排在前十位的依次为弦脉、数脉、滑脉、沉脉、浮脉、细脉、迟脉、虚脉、缓脉、结脉;（3）130位中医师描述的涩脉特征涉及不流利、脉形细、脉率慢、脉律不齐、脉力不足、脉位沉、脉形短等8个方面的特征;（4）117位中医师描述的紧脉特征涉及紧张度甚于弦脉、有力、脉率偏快、脉位偏浮等四个方面的特征。结论：为进一步研究脉象客观化问题提供参考依据。     

五种化感物质对人参幼根皂苷含量的影响

采用比色法测定了人参根系分泌物中苯甲酸、邻苯二甲酸二异丁酯、丁二酸二异丁酯、棕榈酸和2,2-二(4-羟基苯基)丙烷在1mg·L-1、0.1mg·L-1和0.01mg·L-1浓度下对人参幼根总皂苷含量的影响。试验结果表明,酚酸类化感物质能够降低人参皂苷含量,且随浓度增加,人参皂苷含量明显减少。研究结果说明酚酸类化感物质不仅对人参种子萌发、幼苗生长具有自毒作用,而且对人参有效成分的含量有显著影响。     

电针配合梅花针叩刺后拔罐治疗肩手综合征疗效观察

目的:比较电针配合梅花针叩刺后拔罐与单纯电针治疗中风后肩手综合征的疗效差异.方法:70例随机分为治疗组及对照组各35例.治疗组取天宗、肩髃、肩髎等穴针刺,配合取疼痛、痉挛处梅花针叩刺后拔罐.对照组采用单纯电针疗法,通过上肢关节活动度、疼痛等指标判断其效果.结果:总有效率治疗组88.6%,对照组80.0%,两组比较有显著性差异(P<0.05).结论:电针配合梅花针叩刺后拔罐治疗中风后肩手综合征疗效显著.     

古籍数字化的保真问题

保真问题是在古籍数字化过程中,业内专家面临解决数字化古籍使用者所关心的首要问题。本文从实践出发,探讨了数字化过程中的古籍载体转换以及古籍发布形式两个环节上面临的保真问题,并提出了解决保真问题的新思路。     

试述仲景温心阳法在治疗津液相关疾病中的运用

津液的代谢,除了与肺脾肾三脏相关以外,尚与心联系密切。若心阳不足,则可能发生阳虚自汗、水气上冲、表证水停和阳虚水泛等津液相关疾病。因此,在此类疾病的诊治中,只要能抓住心阳（气）虚弱这一共有的病理机制,根据异病同治法则来温煦心阳,常可事半功倍。     

Quantitative analysis of chemical constituents in different commercial parts of Notopterygium incisum by HPLC–DAD–MS

Ethnopharmacological relevance

Notopterygium incisum used as an antirheumatic and analgesic medicine for the treatment of rheumatism, headache, cold, etc., is not only a traditional Qiang medicine, but also a traditional Tibetan remedy for thousands of years.

Aim of the study

In order to use this herbal medicine in a more effective way, in the present investigation, we analyzed the main chemical constituents of different commercial parts as well as fibrous roots of Notopterygium incisum.

Materials and methods

All crude Notopterygium incisum samples used in this study were collected in Sichuan province of China. We used HPLC-DAD-MS to analyze quantitatively the main chemical constituents of different commercial parts as well as fibrous roots of Notopterygium incisum.

Results

According to different commercial parts, 10 Notopterygium incisum samples collected from two main production areas have been investigated, and four main constituents have been quantitatively analyzed.

Conclusion

The quality of rhizomes is better than that of main roots. Unexpectedly, the content of two main constituents, notopterol and isoimperaorin, in fibrous roots is higher than any other parts of Notopterygium incisum. This result implied that the fibrous root may also have value, and we should study it to explore its potential.