Expression pattern of the CCAAT/enhancer-binding protein C/EBP-beta in gestational trophoblastic disease.

The CCAAT/enhancer-binding protein (C/EBP) family consists of several factors that are important regulators of intracellular processes and hormone action. C/EBP-beta, the most important member of the C/EBP family, was shown recently to be expressed in the normal human placenta where it is localized in villous syncytiotrophoblast and in the extravillous (intermediate) trophoblast but not the villous cytotrophoblast. The purpose of this study was to investigate the expression pattern of C/EBP-beta in gestational trophoblastic disease (GTD) which has not been studied so far. We used immunohistochemistry on a total of 15 cases of GTD including nine complete hydatidiform moles, one placental site nodule (PSN), one placental site trophoblastic tumor (PSTT), and four choriocarcinomas. All our tested specimens showed positivity for C/EBP-beta. The strongest C/EBP-beta expression could be observed in villous syncytiotrophoblast and in the trophoblast proliferations on the villous surface of hydatidiform moles; villous cytotrophoblast was negative. The PSN also showed positive nuclear staining but the expression was not as strong as it was in the hydatidiform moles and the total amount of stained cells was the lowest of all GTD. The PSTT also showed immunoreactivity but with a weaker and more heterogeneous staining than in the choriocarcinomas. The specific expression pattern of C/EBP-beta in GTD indicate that C/EBP-beta could potentially be an additional marker of such lesions.     

夜间电子生物阻抗测量装置对阴茎勃起功能障碍的检测

目的 :探讨夜间电子生物阻抗测量装置 (NEVA)在阴茎勃起功能障碍的诊断中的应用价值。方法 :对 4 1例主诉阴茎勃起功能障碍者 (障碍组 ) 19例主诉无勃起功能障碍但有射精障碍者 (无障碍组 )进行NEVA检测。结果 :障碍组夜间试验中的夜间勃起的次数、阴茎勃起的最大体积改变、最长持续时间等客观指标均要差于无障碍组 ,差异有统计学意义 (P <0 .0 1)。结论 :NEVA为非侵入性检测、可鉴别心理性阴茎勃起功能障碍和严重的器质性勃起功能障碍 ,且对勃起功能障碍的定性和定量具有一定的客观性。     

基于混合遗传算法的心脏病决策支持系统研究

将遗传算法和 BP算法相结合 ,建立了一个基于混合遗传算法的心脏病决策支持系统来鉴别诊断五种常见心脏病 (冠心病 ,高血压性心脏病 ,风湿性心脏病 ,慢性肺原性心脏病和先天性心脏病 )。一个含有 35 2份心脏病的数据库用来构建和测试了该系统。实验结果表明 ,构建的系统对这五种心脏病均有较好的诊断识别率 ,系统的平均识别准确性达 90 .6 % ,各疾病的用户准确性和程序准确性均大于 85 .0 % ,表现出良好的心脏病的临床诊断决策支持能力     

高校科技创新工作的若干问题及对策

我国高等学校的科技队伍不断发展和壮大 ,在我国的科技事业发展中具有举足轻重的地位 ,高等学校的科技创新对推进我国国家创新体系的建设起着越来越重要的作用。但高校科学研究工作中存在一些困难和问题 ,必须采用改革现行管理体制和运行机制等对策予以解决     

枢椎齿突骨折的治疗进展

枢椎齿突骨折是一种累及寰枢椎区稳定性的严重损伤 ,人们对它的认识是不断变化和深入的。近年来 ,出现了很多新的治疗观念和方法 ,作者对此作一综述     

顺铂持续压力下HepG2存活细胞中P53、C-myc、Bax、Bcl-2的变化及意义

目的动态观察HepG2细胞在顺铂压力下存活细胞中P53、C-myc、Bax、Bcl-2等的变化,进一步探讨HepG2顺铂耐药的机制.方法采用免疫组化法和RT-PCR法测定在顺铂压力下存活HepG2细胞内凋亡和抗凋亡因子P53、C-myc、Bax、Bcl-2、NF-κB的变化.结果在顺铂压力下存活HepG2细胞P53、C-myc等凋亡相关因子无明显变化.Bcl-2持续升高,Bax逐渐下降,Bcl-2/Bax的比率值呈进行性升高.NF-κB mRNA在7 d后出现明显阳性.结论在顺铂持续压力下HepG2细胞的存活机制与Bcl-2持续升高、Bax逐渐下降有关,NF-κB mRNA的阳性表达可能与细胞存活的后续事件有关.     

Naturlatexallergie

As a consequence of AIDS prevention strategies, the use of powdered disposable latex gloves in hospitals and private medical practices has increased conspicuously in the past 10 years, and allergic reactions to natural latex have become a frequently observed problem. The clinical picture of type I allergies (immediate hypersensitivity) to natural latex ranges from slight, local allergic symptoms (urticaria, rhinitis, and conjunctivitis) to asthmatic complaints up to severest anaphylactic reactions. In addition to health care employees, patients are being affected with an increasing tendency during a variety of surgical and other medical interventions. Due to its enormous hygienic and economic significance, natural latex has become one of the most frequently studied sources of allergies. Efforts were undertaken to identify the causes and triggering factors, to develop diagnostic tools and continue to improve upon them, and to establish preventive measures and implement them successfully.     

田径运动员最大摄氧量和无氧功测试中血乳酸的应用

目的:研究田径运动员最大摄氧量和Wingate测试后血乳酸的变化,分析血乳酸对测试结果的意义。方法:测试田径短跑运动员36例和中长跑运动员29例的最大摄氧量和Wingate无氧功,以及测试后的血乳酸值。结果:最大摄氧量测试后血乳酸数值为9～11mmol/L左右,摄氧量与乳酸值没有直接相关关系,但不同项目之间有一定的差异;Wingate测试后血乳酸数值一般大于12mmol/L,测试的主要评价指标与乳酸值都有较高的相关关系。结论:血乳酸值对于最大摄氧量测试意义不大,但对于Wingate测试可以作为一项辅助评价指标。     

去骨瓣减压术治疗大面积脑梗死的短期疗效评价

目的:评价去骨瓣减压术治疗大面积脑梗死的效果。方法:制定入选和排除标准,统一术式,比较术前、术后不同时期的神经功能变化,并对存活病例进行随访(6个月),评价术后3和6个月时的预后评分(GOS)和BarthelIndex(BI)的变化。结果:按入选标准行去骨瓣减压术26例,术后死亡率为30.8%。术前昏迷评分GCS对决定手术时机有指导作用。共随访14例患者,术后3和6个月GOS分别为3.6±0.8和4.0±0.8,与出院时GOS评分比差异有统计学意义。术后3和6个月BI分别为68.9±29.4和77.5±28.3,其中术后6个月BI>60者占85.7%。结论:对保守治疗无效的大面积脑梗死患者,去骨瓣减压术不仅可作为一种“救命”手术,而且多数存活病例恢复较好。合理选择手术适应证、及时把握手术时机以及充分手术减压可能是影响预后的重要因素。     

烤瓷贴面修复20例临床观察

目的评价烤瓷贴面的修复效果。方法选择20例患者制作烤瓷贴面92个。复诊时对烤瓷贴面的边缘适合性,边缘着色及颜色进行评价;同时采用牙龈出血指数和菌斑指数评价治疗前后的牙周状况。观察时间为12～18个月。结果5颗牙烤瓷贴面失败,成功率为95.7%;烤瓷贴面治疗前后牙龈出血指数无显著性差异(P>0.05),治疗后菌斑指数降低(P<0.01)。结论烤瓷贴面具有较好的治疗效果。