全文获取类型
收费全文 | 2144篇 |
免费 | 169篇 |
国内免费 | 14篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 110篇 |
妇产科学 | 39篇 |
基础医学 | 277篇 |
口腔科学 | 36篇 |
临床医学 | 236篇 |
内科学 | 579篇 |
皮肤病学 | 14篇 |
神经病学 | 191篇 |
特种医学 | 50篇 |
外科学 | 246篇 |
综合类 | 80篇 |
预防医学 | 236篇 |
眼科学 | 20篇 |
药学 | 109篇 |
中国医学 | 3篇 |
肿瘤学 | 86篇 |
出版年
2022年 | 14篇 |
2021年 | 36篇 |
2020年 | 18篇 |
2019年 | 27篇 |
2018年 | 39篇 |
2017年 | 26篇 |
2016年 | 36篇 |
2015年 | 23篇 |
2014年 | 54篇 |
2013年 | 78篇 |
2012年 | 137篇 |
2011年 | 120篇 |
2010年 | 75篇 |
2009年 | 64篇 |
2008年 | 128篇 |
2007年 | 144篇 |
2006年 | 123篇 |
2005年 | 141篇 |
2004年 | 112篇 |
2003年 | 79篇 |
2002年 | 100篇 |
2001年 | 66篇 |
2000年 | 56篇 |
1999年 | 50篇 |
1998年 | 28篇 |
1997年 | 31篇 |
1996年 | 27篇 |
1995年 | 26篇 |
1994年 | 18篇 |
1993年 | 10篇 |
1992年 | 41篇 |
1991年 | 37篇 |
1990年 | 36篇 |
1989年 | 21篇 |
1988年 | 29篇 |
1987年 | 31篇 |
1986年 | 28篇 |
1985年 | 13篇 |
1984年 | 17篇 |
1983年 | 14篇 |
1982年 | 8篇 |
1981年 | 10篇 |
1979年 | 22篇 |
1978年 | 8篇 |
1976年 | 13篇 |
1975年 | 16篇 |
1974年 | 13篇 |
1972年 | 8篇 |
1970年 | 9篇 |
1969年 | 6篇 |
排序方式: 共有2327条查询结果,搜索用时 146 毫秒
111.
Rabies virus (RV) readily infects cattle and causes a fatal neurological disease. A stable vaccine, which does not require the maintenance of a cold chain and that is administered once to elicit lifelong immunity to rabies would be advantageous. The present study describes the construction of a live recombinant lumpy skin disease virus (LSDV) vaccine, expressing the glycoprotein of rabies virus (RG) and assessment of its ability to generate a humoral and cellular immune response against rabies virus in cattle. Cattle inoculated with the recombinant virus (rLSDV-RG) developed humoral immunity that was demonstrated in ELISA and neutralisation assays to RV. High titres of up to 1513IU/ml of RV neutralising antibodies were induced. In addition, peripheral blood mononuclear cells from rLSDV-RG-immunised animals demonstrated the ability to proliferate in response to stimulation with inactivated RV, whereas the animal vaccinated with wild type LSDV did not. This recombinant vaccine candidate thus has the potential to be used in ruminants as a cost-effective vaccine against both lumpy skin disease (LSD) and rabies. 相似文献
112.
Focal nodular hyperplasia inducing hepatic vein obstruction 总被引:3,自引:0,他引:3
Rangheard AS Vilgrain V Audet P O'Toole D Vullierme MP Valla D Belghiti J Menu Y 《AJR. American journal of roentgenology》2002,179(3):759-762
OBJECTIVE: The records of 10 patients with focal nodular hyperplasia inducing intrahepatic vein obstruction were reviewed. The purpose of this study was to describe and emphasize the imaging features of these findings. CONCLUSION: Focal nodular hyperplasia may be responsible for hepatic vein obstruction with hepatic vein collaterals. The relatively large size and central location of the lesions seem to play important roles in the obstruction of the hepatic veins. 相似文献
113.
Background
To assess the feasibility of offering health promotion and preventive medicine initiatives in primary care. 相似文献114.
Hypertolerance to morphine in G(z alpha)-deficient mice 总被引:2,自引:0,他引:2
Hendry IA Kelleher KL Bartlett SE Leck KJ Reynolds AJ Heydon K Mellick A Megirian D Matthaei KI 《Brain research》2000,870(1-2):10-19
Our laboratory has generated a mouse deficient in the alpha (alpha) subunit of the G protein, G(z), (G(z alpha)) gene and we have examined the involvement of G(z alpha) in spinal and supraspinal analgesia and tolerance mechanisms. Spinal analgesia was tested by the response times to heat or cold tail flick times in a water bath at 50 degrees C or -5 degrees C and supraspinal analgesia was tested by the times for paw licking and jumping from a plate at 52 degrees C or 0.5 degrees C. Tolerance to morphine was induced in wild type and G(z alpha)-deficient mice over a 5 day period and the behavioral tests were performed daily. The tail flick reaction times to both hot and cold stimuli did not differ between the wild type and G(z alpha)-deficient mice. Analysis of the reaction times from the hot and cold plate tests showed the G(z alpha)-deficient mice developed tolerance to morphine to a greater degree and at a faster rate than wild type mice. Opioid binding assays were performed on synaptic membranes prepared from naive and morphine tolerant wild type and G(z alpha)-deficient brains. No changes in the affinity of morphine for its receptor or in the density of mu and delta opioid receptors were found between the two groups of mice in the naive or morphine tolerant state. This indicates that the absence of G(z alpha) does not affect opioid receptor affinity or receptor up or down regulation. Our results suggest that the presence of G(z alpha) delays the development of morphine tolerance and represents a possible therapeutic target for improving the clinical use of morphine. 相似文献
115.
McLennan JD Jansen-McWilliams L Comer DM Gardner WP Kelleher KJ 《Journal of developmental and behavioral pediatrics : JDBP》1999,20(1):24-30
Clinicians' management of children with psychosocial problems may vary with their attitudes and beliefs. However, we lack sound instruments to assess these factors. This study examined the psychometric properties of the Physician Belief Scale (PBS). A modified version of the PBS was sent to clinicians who participated in two primary care research networks. Using factor analysis, we reduced the PBS to 14 items and 2 subscales. Cronbach's alpha values were high. Female clinicians, those using DSM-IV, and those having completed training earlier rated themselves as more psychosocially oriented. Identification and treatment of psychosocial problems were significantly related to scores on the Belief and Feeling subscale. The PBS provided only a modest amount of information regarding primary care practices. It is not clear if these limitations are attributable to the instrument or the many other barriers to effective psychosocial care. 相似文献
116.
A.M. Pleil P.R. Reese C.J. Kelleher G.J. Okano 《HEPAC Health Economics in Prevention and Care》2001,2(2):69-75
Overactive bladder (OAB) imposes a burden on many aspects of a patient's life. The health-related quality of life (HRQOL) of OAB patients has been shown to be lower than healthy or control patients. A goal of successful treatment is to improve the HRQOL of OAB patients. With newer OAB treatments becoming available, HRQOL is an important outcome to measure. Thus, the objective of this study was to compare the HRQOL of patients being treated with tolterodine immediate-release (IR) versus placebo for OAB. The King's Health Questionnaire (KHQ) and the Medical Outcomes Study Short Form 36 (SF-36) were administered at baseline and end of treatment (12 weeks) in a randomized, parallel groups, placebo-controlled, double-blind, multinational study designed to compare the clinical efficacy and safety of tolterodine to placebo in the treatment of OAB. At end of treatment KHQ domains selected a priori as primary HRQOL endpoints (incontinence impact and role limitations) significantly improved (PА.0001) with tolterodine IR. Domains selected a priori as secondary HRQOL or symptom endpoints (physical limitations, emotions, sleep and energy, severity (coping) measures, and symptom severity) were also significantly improved (PА.0074) following treatment with tolterodine IR. The tolterodine IR group had less severity of individual symptoms and significantly better patient rating of bladder control than the placebo group at end of treatment. As expected, the SF-36 did not detect treatment differences. In conclusion, many aspects of HRQOL, as measured by the KHQ, significantly improved with tolterodine IR treatment. These HRQOL improvements were consistent with clinical efficacy results. The findings demonstrate that tolterodine IR patients experience overall improvement in their condition that resulted in important impacts on their HRQOL. 相似文献
117.
Sequence tag identification of intact proteins by matching tanden mass spectral data against sequence data bases.
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
E M?rtz P B O'Connor P Roepstorff N L Kelleher T D Wood F W McLafferty M Mann 《Proceedings of the National Academy of Sciences of the United States of America》1996,93(16):8264-8267
Molecular and fragment ion data of intact 8- to 43-kDa proteins from electrospray Fourier-transform tandem mass spectrometry are matched against the corresponding data in sequence data bases. Extending the sequence tag concept of Mann and Wilm for matching peptides, a partial amino acid sequence in the unknown is first identified from the mass differences of a series of fragment ions, and the mass position of this sequence is defined from molecular weight and the fragment ion masses. For three studied proteins, a single sequence tag retrieved only the correct protein from the data base; a fourth protein required the input of two sequence tags. However, three of the data base proteins differed by having an extra methionine or by missing an acetyl or heme substitution. The positions of these modifications in the protein examined were greatly restricted by the mass differences of its molecular and fragment ions versus those of the data base. To characterize the primary structure of an unknown represented in the data base, this method is fast and specific and does not require prior enzymatic or chemical degradation. 相似文献
118.
119.
The absorption of alpha-tocopherol in man 总被引:3,自引:0,他引:3