Bonded resin sealant on smooth surface dental enamel--an in vitro study

The aim of this study was to in vitro evaluate the micro leakage of a fissure sealant on sound and demineralized enamel using thermo cycling. The effect of the sealing procedure on sound and demineralized enamel were assessed on human premolars that were divided into one Control group and two Test groups. The Control group served as a baseline reference for the subsequent test procedures. The crowns of five teeth were covered with wax leaving three separate windows on the buccal surfaces. The enamel in one window was acid-etched, in the second window demineralized, and in the third window demineralized and subsequently acid-etched. In Test group 1, containing ten teeth, a defined enamel area on the buccal surfaces was acid-etched and sealed. The sealed area and a border of sound enamel around it were subjected to the demineralizing procedure. In Test group 2, a defined enamel window of 13 teeth was demineralized and subsequently etched and sealed. The sealed area and a border of surrounding enamel were subjected to a new cycle of demineralization. The longitudinal, bucco-lingual sections were examined in polarized light. When placed on acid-etched surfaces, the resin adhered firmly to the enamel with no evidence of demineralization or enlargement of previously demineralized areas underneath the sealants.     

高血压脑出血病人术后护理

高血压性脑出血患者多发病急、来势凶猛,是造成患者死亡和致残的常见疾病;而手术是抢救患者生命的主要方法,28例患者经过我科手术治疗,术前、术后精心护理,治疗结果良好.     

The effects of topically applied polyNIPAM-based nanogels and their monomers on skin cyclooxygenase expression,ex vivo

Stimulus-responsive nanogels have potential as carriers for drugs targeting the skin. It is important to estimate the biocompatibility of such materials with the skin since they are directly in contact upon application and may induce irritation or inflammation. In the current work, blank (drug-free) polyN-isopropylacrylamide (polyNIPAM), poly(NIPAM copolymerized butyl acrylate) [poly(NIPAM-co-BA)], and poly(NIPAM copolymerized with 5% w/v acrylic acid) [poly(NIPAM-co-AAc)(5%)] nanogels were dosed onto freshly excised full-thickness porcine ear skin and the effects on the expression of cyclooxygenase-2 (COX-2) determined ex vivo by Western blotting. Modulated COX-2 expression was indicative that the material had penetrated the skin and keratinocytes of the viable epidermis. The poly(NIPAM-co-BA) nanogel was found to exert a proinflammatory response when applied topically, as reflected by 67% higher COX-2 expression relative to the control treatment (p = 0.0035). The data obtained for the poly(NIPAM-co-AAc)(5%) nanogel, on the other hand, indicated no significant modulation in the expression of COX-2 (p = 0.1578), suggesting the particles are compatible with skin. This was even the case in the presence of co-administered aqueous citric acid solution. Overall the data support the use of the multi-responsive poly(NIPAM-co-AAc)(5%) nanogel for triggered or controlled topical drug delivery applications.     

改良封闭式负压引流技术在骨外科创伤治疗中的应用

目的：对改良封闭式负压引流技术在骨外科创伤治疗中的应用进行探讨。方法整群选取2012年6月-2013年6月于该院进行治疗的104例骨外科创伤患者,将其随机分为对照组51例与观察组53例。对照组使用常规封闭式负压引流技术治疗方法,观察组给予经改良后的封闭式负压引流技术治疗,即在常规VSD敷料中加入1根冲洗管,进行持续灌注冲洗。观察并评价两组的疗效、敷料更换次数、平均每次敷料的有效时间和患者医疗的费用。结果观察组总有效率为96.2%,明显高于对照组总有效率的85.3%,差异有统计学意义(P<0.05);观察组在更换敷料次数、敷料平均维持时间以及治疗费用上均明显优于对照组,差异有统计学意义(P<0.05)。结论改良封闭式负压引流技术在骨外科创伤治疗中的临床效果明显好于用常规治疗方法,临床医生治疗骨外科创伤治疗可以优先考虑该方法。     

γH2AX和53BP1蛋白在肺正常上皮细胞DNA氧化损伤反应中的表达

目的探讨肺正常上皮细胞(human bronchial epithelial cells,HBE)中磷酸化组蛋白H2AX(phosphorylated H2AX,γH2AX)和p53结合蛋白1(p53-Binding protein 1,53BP1)在DNA氧化损伤反应中的表达。方法用0、25、50、100、200、400μmol/L过氧化氢(H2O2)处理HBE细胞1 h,对其造成氧化损伤引起DNA双链断裂(double strand breaks,DSBs);CCK-8比色法检测H2O2对HBE细胞活性的影响,流式细胞术检测细胞凋亡情况,荧光显微镜检测HBE细胞核内γH2AX和53BP1蛋白的聚集情况,western blot检测γH2AX、53BP1、BRCA1蛋白的表达水平。结果与对照组比较,25μmol/L H2O2处理组细胞活性(1.07±0.01)升高,50、100、200、400μmol/L H2O2处理组细胞活性(分别为0.97±0.01,0.96±0.01,0.95±0.01和0.94±0.01)降低,差异具有统计学意义(F=50.35,P<0.01)。细胞凋亡检测结果显示,与对照组[(4.65±0.32)%]比较,50、100、200、400μmol/L H2O2处理组细胞凋亡率[分别为(7.54±0.57)%、(7.84±0.68)%、(8.40±0.50)%和(14.03±1.03)%]升高(F=35.879,P<0.01)。与对照组比较,经25、50、100、200、400μmol/L H2O2处理后γH2AX的平均荧光强度升高(F=223.97,P<0.01),而经50、100、200、400μmol/L H2O2处理后53BP1的平均荧光强度降低(F=117.78,P<0.01);western blot结果显示,随着H2O2浓度升高,γH2AX蛋白表达水平升高,BRCA1和53BP1的表达水平下降,差异均具有统计学意义(F分别为96.20,11.55,21.92,P均<0.01)。结论在H2O2导致HBE细胞的氧化损伤中,γH2AX可作为DNA氧化损伤标志物,但53BP1的表达下降提示HBE细胞可能通过其他途径进行DNA氧化损伤的修复。