渐进性肌肉放松训练联合引导性想象放松训练在肺癌患者中的应用效果

目的 探讨渐进性肌肉放松训练联合引导性想象放松训练在肺癌患者中的应用效果.方法 随机数字表法将100例肺癌患者分为对照组和研究组,每组50例,对照组患者接受渐进性肌肉放松训练,研究组患者接受渐进性肌肉放松训练联合引导性想象放松训练.干预前后,采用焦虑自评量表(SAS)和抑郁自评量表(SDS)比较两组患者的焦虑、抑郁情绪...     

Investigating the Impact of the New York State Flavor Ban on e-Cigarette–Related Discussions on Twitter: Observational Study

BackgroundOn May 18, 2020, the New York State Department of Health implemented a statewide flavor ban to prohibit the sales of all flavored vapor products, except for tobacco or any other authorized flavor.ObjectiveThis study aims to investigate the discussion changes in e-cigarette–related tweets over time with the implementation of the New York State flavor ban.MethodsThrough the Twitter streaming application programming interface, 59,883 e-cigarette–related tweets were collected within the New York State from February 6, 2020, to May 17, 2020 (period 1, before the implementation of the flavor ban), May 18, 2020-June 30, 2020 (period 2, between the implementation of the flavor ban and the online sales ban), July 1, 2020-September 15, 2020 (period 3, the short term after the online sales ban), and September 16, 2020-November 30, 2020 (period 4, the long term after the online sales ban). Sentiment analysis and topic modeling were conducted to investigate the changes in public attitudes and discussions in e-cigarette–related tweets. The popularity of different e-cigarette flavor categories was compared before and after the implementation of the New York State flavor ban.ResultsOur results showed that the proportion of e-cigarette–related tweets with negative sentiment significantly decreased (4305/13,246, 32.5% vs 3855/14,455, 26.67%, P<.001), and tweets with positive sentiment significantly increased (5246/13,246, 39.6% vs 7038/14,455, 48.69%, P<.001) in period 4 compared to period 3. “Teens and nicotine products” was the most frequently discussed e-cigarette–related topic in the negative tweets. In contrast, “nicotine products and quitting” was more prevalent in positive tweets. The proportion of tweets mentioning mint and menthol flavors significantly increased right after the flavor ban and decreased to lower levels over time. The proportions of fruit and sweet flavors were most frequently mentioned in period 1, decreased in period 2, and dominated again in period 4.ConclusionsThe proportion of e-cigarette–related tweets with different attitudes and frequently discussed flavor categories changed over time after the implementation of the New York State ban of flavored vaping products. This change indicated a potential impact of the flavor ban on public discussions of flavored e-cigarettes.     

正常中国汉人红细胞中儿茶酚氧位甲基转移酶的活性测定

目的测定正常中国人群红细胞儿茶酚氧位甲基转移酶(COMT)活性浓度值,为研究COMT活性变化在疾病诊断中的应用提供依据。方法采用高效液相色谱法测定279名正常健康人(其中男性134例,女性145例)红细胞中COMT的活性浓度,用SPSS统计软件对性别间测定结果进行检验比较。结果男性和女性红细胞中COMT活性浓度分别为:(16.5±7.4)、(15.5±5.3)nmol.mL RBC-1.h-1。活性频率分布男性在6.0~37.4nmol.mL RBC-1.h-1,女性为5.4~37.2 nmol.mL RBC-1.h-1,男女之间COMT活性浓度的差异无统计学意义(P>0.05)。结论本法灵敏,简便,准确,重复性好,且测定结果与国外一些报道一致,适用于临床应用测定研究。本结果可作为中国健康人红细胞COMT活性正常值及今后研究的重要参考数据。     

Cell-penetrating peptide-doxorubicin conjugate loaded NGR-modified nanobubbles for ultrasound triggered drug delivery

A new drug-targeting system for CD13⁺ tumors has been developed, based on ultrasound-sensitive nanobubbles (NBs) and cell-permeable peptides (CPPs). Here, the CPP-doxorubicin conjugate (CPP-DOX) was entrapped in the asparagine–glycine–arginine (NGR) peptide modified NB (CPP-DOX/NGR-NB) and the penetration of CPP-DOX was temporally masked; local ultrasound stimulation could trigger the CPP-DOX release from NB and activate its penetration. The CPP-DOX/NGR-NBs had particle sizes of about 200?nm and drug entrapment efficiency larger than 90%. In vitro release results showed that over 85% of the encapsulated DOX or CPP-DOX would release from NBs in the presence of ultrasound, while less than 1.5% of that (30?min) without ultrasound. Cell experiments showed the higher cellular CPP-DOX uptake of CPP-DOX/NGR-NB among the various NB formulations in Human fibrosarcoma cells (HT-1080, CD13⁺). The CPP-DOX/NGR-NB with ultrasound treatment exhibited an increased cytotoxic activity than the one without ultrasound. In nude mice xenograft of HT-1080 cells, CPP-DOX/NGR-NB with ultrasound showed a higher tumor inhibition effect (3.1% of T/C%, day 24), longer median survival time (50 days) and excellent body safety compared with the normal DOX injection group. These results indicate that the constructed vesicle would be a promising drug delivery system for specific cancer treatment.     

Investigation of Potential Use of Soybean Protein Isolate–Chinese Bayberry Tannin Extract Cross-Linked Films in Packaging Applications

The possibility of using commercial bayberry tannin (BT) from a Chinese source as a cross-linker and functional additive to develop soybean protein isolate (SPI)-based films was explored in this study by using the solvent casting method. In particular, the impacts of BT loading on the tensile strength, microstructure, thermal stability, water resistance and antioxidant capacity were fully investigated. The results reveal that SPI incorporated with BT yielded a phenolic–protein hybrid whose relevant films exhibited an improvement in tensile strength of around two times greater compared with native SPI as a result of the formed interactions and covalent cross-links, which could be proven using FTIR spectroscopy. The introduction of BT also led to the compact microstructure of SPI–BT films and enhanced the thermal stability, while the water vapor permeability was reduced compared with the control SPI film, especially at high loading content of tannin. Additionally, the use of BT significantly promoted the antioxidant capacity of the SPI-based films according to DPPH radical scavenging assay results. On this basis, Chinese bayberry tannin is considered a promising natural cross-linker and multifunctional additive that can be dedicated to developing protein-derived films with antioxidant activity for food packaging applications.     

鼓室硬化和非鼓室硬化中耳炎抗氧化能力的差异性研究

目的 了解鼓室硬化和非鼓室硬化中耳炎患者在抗氧化物质超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalnse,CAT)活性及过氧化反应产物丙二醛(malondialdehyde,MDA)浓度方面的差异,以进一步探讨鼓室硬化形成的机制.方法 回顾分析56例(56耳)中耳炎、中耳炎后遗症手术患者资料,分成两组:鼓室硬化组26耳,非鼓室硬化组30耳.测定所有患者血浆、红细胞中SOD、CAT活力及血浆、中耳组织中MDA浓度.结果 分析用秩和检验及相关分析.结果 两组血浆中MDA含量差异无统计学意义.鼓室硬化组中耳组织中MDA含量较高,差异有统计学意义(P<0.05).两组红细胞中SOD和CAT活力差异无统计学意义.鼓室硬化组血浆中的SOD和CAT活力较非鼓室硬化组低.差异有统计学意义.鼓室硬化组术前的听力平均值与其各检测项目所测得的MDA、SOD、CAT值均不存在相关(P>0.05).结论 鼓室硬化患者可能存在局部氧自由基浓度增高和抗氧化物质活性降低的情况.局部氧自由基浓度增高,血液抗氧化物质无法进入中耳组织,可能是形成鼓室硬化的病理基础.     

Safety and efficacy of anti-EGFR monoclonal antibody (SCT200) as second-line therapy in advanced esophageal squamous cell carcinoma

Objective: The mainstay treatment of esophageal squamous cell carcinoma(ESCC) involves chemotherapy and immunotherapy. However, alternative therapies are required for patients who are refractory or intolerant to existing therapies.Methods: In this single-arm, multicenter, open-label phase Ib study, 30 patients received an intravenous infusion of SCT200, an antiepidermal growth factor receptor(EGFR) monoclonal antibody, 6.0 mg/kg once a week for 6 weeks, followed by 8.0 mg/kg once every 2 weeks u...     

Construction of developmentally inspired periosteum-like tissue for bone regeneration

The periosteum, a highly vascularized thin tissue, has excellent osteogenic and bone regenerative abilities. The generation of periosteum-mimicking tissue has become a novel strategy for bone defect repair and regeneration, especially in critical-sized bone defects caused by trauma and bone tumor resection. Here, we utilized a bone morphogenetic protein-2(BMP-2)-loaded scaffold to create periosteum-like tissue(PT) in vivo, mimicking the mesenchymal condensation during native long bone developmen...     

中医治疗抑郁症的辨证思路

因为患者的心境不好会造成抑郁症的产生,抑郁症的发病主要是根据患者的心境进行判断,因此它属于一种精神上的疾病.近年来,人类的疾病正在不断地发生着变化,因此要对抑郁症进行深度的解析,以求为抑郁症治疗提供一定借鉴.本文就相关的抑郁症的治疗进行分析,主要从患者的心理因素,抑郁症的发展变化上根据个体的差异不同来实现对不同人群的研究,对治疗过程中所产生的一些中医辨证治疗思路进行探究.