肺隔离症的影像诊断和介入治疗

目的探讨肺隔离症的影像学表现及介入治疗的应用价值. 资料与方法对5例肺隔离症患者的X线平片、CT、MRI表现进行分析,并对隔离的肺组织的供血动脉进行栓塞. 结果 X线平片主要表现为囊状或团状高密度影及支气管扩张样改变,CT、MRI可发现部分异常供血动脉,血管造影均能发现供血动脉,经异常供血动脉采用不锈钢圈栓塞后临床症状逐渐减轻、消失,随访6个月～1年,症状未再复发. 结论在影像诊断方面,X线平片难以确诊,CT、MRI可部分确诊,而DSA检查是肺隔离症诊断的金标准.经异常供血动脉栓塞治疗肺隔离症安全,患者痛苦小,并发症少,是一种有效的治疗方法.     

腰椎间盘突出症的针灸治疗近况及作用机理探讨

腰椎间盘突出症（1umbar intervertebral discprotrusion，LIPD）是一种临床上常见的疾病，好发于青壮年，发病急骤，病程迁延反复，临床主要表现为腰腿疼痛，下肢活动障碍，严重者可下肢瘫痪，给病人带来极大的痛苦及经济负担。目前关于LIPD的治疗的研究报道很多，总的来说可以分为两大类：手术治疗与非手术治疗，在非手术治疗方法的报道中针灸及以针灸为主的综合疗法占有非常重要地位，西医界一些著名专家也把针灸、触发点（triggerpoint）刺激、药物局部注射视为LIPD最常规的治疗方法。本文就LIPD的针灸治疗近况及作用机理作如下综述。     

小剂量长春新碱对小鼠M1细胞的分化诱导作用研究

目的：利用培养小鼠髓性白血病（M1细胞）来检测小剂长春新碱（VCR）对小鼠M1细胞分化诱导功能。方法：分观察组对照组，观察组使用VCR1次5ug后进行吞噬功能实验，及对细胞形态的观察与空白对照组比较。结果：发现使用小剂量长春新碱后小鼠M1细胞诱变细胞增多，吴噬功能增强，结论：说明小剂量长春新碱对培养小鼠M1细胞有一定诱导功能，诱导其向粒细胞、吞噬细胞方向转化，在临床上白血病的治疗有一定的作用。     

水泥型羟基磷灰石人工骨的制备及性能分析

Objective: To prepare hydroxyapatite cement (or calcium phosphate cement,CPC) and analyze its capability. Methods: Tetracalcium phospluge (TTCP ) was prepared by the method of high heat. TTCP reacted with in simulated body situation and produced CPC. Its capability was analyzed by scanning electron microscopy ( SEM), X-ray diffraction( XRD). Its density, absorbing water coefficient, macroporosity and compressive strength were measured also. Results: The main element of CPC is hydroxyapatile (HA), its microstructure comprised of petal crys-tals. The diameter of micropore was 4-10μm, density was 1. 922 g/cm^3, macroporosity was 29. 777%, absorbing coefficient was 15. 503%, compressive strength was 42.70 Mpa. Conclusion: This CPC has three-dimensional spatial structure, its strength meets the need of cancellous bone grafting.     

The characteristics of Hep-2 cell with multiple drug resistance induced by Taxol.

OBJECTIVE: To investigate the characteristics of Hep-2 cell with multidrug resistance (MDR) induced by Taxol. STUDY DESIGN: Hep-2 cells were exposed in stepwise escalating concentration of Taxol to develop the resistant cell line-Hep-2T. Cell cycle distribution, apoptosis, and rhodamine accumulation were studied through flow cytometry. The MDR1 and MRP1 genes were detected through real-time quantitative RT-PCR, and the corresponding proteins were detected through Western blotting. RESULTS: The drug resistance of Hep-2T cells to Taxol, doxorubicin, gemcitabine, 5-FU, and cisplatin all increased. The percentage of G0/G1 phase and the antiapoptosis ability increased significantly compared with Hep-2 cells. Both MDR1 and MRP1 also increased at gene and protein level, though MDR1 was more prominent. CONCLUSION: More emphasis should be laid on MDR1/Pgp, the non-Pgp substrate chemotherapeutic agents, and the changes of cell cycle distribution to prevent MDR induced by Taxol. SIGNIFICANCE: These findings may provide theoretical support for the reverse of MDR.     

干细胞动员对骨创伤大鼠免疫功能的保护作用

目的：观察自体干细胞动员对骨创伤大鼠的免疫功能的保护作用，为战创伤后的免疫抑制及继发性感染、系统性炎症反应等治疗提供依据。方法：挤压折断法复制大鼠双后肢股骨骨折模型，于致伤后0、24、48h连续给模型动物肌肉注射GM—CSF20μg／kg，致伤后72h细胞增殖法检测T、B淋巴细胞活性，ELISA法检测血清IL-1、IL-2、IFN-7、TNF—α浓度，自动系列化分析法检测C3、CA、IgM、IgG浓度，称重法观察脾重与体重的比例变化。结果：股骨骨折模型大鼠致伤后72h，GM—CSF动员骨髓干细胞组大鼠的T、B淋巴细胞活性和血清IFN-γ、IL-2浓度高于对照组（P〈0．01），IL-1、TNF—α浓度低于对照组（P〈0．01），血清C3、CA、IgM、IgG浓度及脾／体重比值高于对照组（P〈0．01）。结论：GM—CSF动员自体干细胞动员对大鼠骨创伤诱导的免疫功能抑制具有保护作用。     

聚丙烯酰胺水凝胶取出同期假体隆乳术分析

目的:介绍一种聚丙烯酰胺水凝胶注射隆乳后取出同时置入假体隆乳的新方法。方法:18例聚丙烯酰胺水凝胶注射隆乳术患者在取出注射物后同期于胸大肌下置入硅凝胶假体。结果:18例患者均取得了良好的效果,外观形态好,无1例出现血肿和感染。结论:聚丙烯酰胺水凝胶注射隆乳后取出注射物同期胸大肌下置入硅凝胶假体是一种可行且效果良好的隆乳矫治方法。     

多种底物在免疫金银染色检测抗核抗体试验中的联合应用

将多种底物联合应用于免疫金银染色检测ANA试验,与各底物单独应用比较,可明显改善检测的敏感性与核型显示。其中,两种鼠脏器印片联合应用的结果可与Hep-2细胞的结果相当,三种及四种鼠脏器印片联合应用的结果可与Hep-2细胞和一种鼠脏器印片联合应用的结果相当,且均不使正常人ANA阳性率明显升高。