利用cDNA微阵列技术筛选成年期与胚胎期睾丸差异表达基因——钙联接蛋白基因

目的 :用cDNA微阵列技术克隆和筛选成年期与胚胎期睾丸差异表达基因 ,以进行睾丸发育及精子发生的调控研究。　方法 :构建人睾丸cDNA芯片 ,分别用正常成人及胚胎睾丸的mRNA探针进行杂交 ,对成人和胚胎睾丸中差异表达的基因进行高流量的比较。　结果 :发现 1条成人睾丸高表达、胚胎低表达的基因———钙联接蛋白 (CLGN)基因。　结论 :运用cDNA微阵列方法可筛选到成年期与胚胎期睾丸差异表达基因     

胎儿脐带缠绕253例分析

目的　探讨胎儿脐带缠绕的临床意义及处理。方法　分析 2 5 3例有脐带缠绕的孕妇的脐带长度、剖宫产率、脐动脉血流S/D的值及对围产儿的影响 ,并与 2 6 9例无脐带缠绕者作比较。结果　 2 5 3例脐带缠绕组中脐带过长者明显高于无脐带缠绕组 (P <0 .0 1)。因脐带缠绕组中脐动脉血流S/D值≥ 3.0的百分率、胎儿宫内窘迫的发生率及新生儿窒息率明显高于对照组 (P <0 .0 1) ,故临床剖宫产率也高于对照组。结论　脐带缠绕可对围产儿造成不良影响 ,临床应提高孕期监测、产程监护 ,从而减少对围产儿的影响 ,降低不必要的剖宫产     

缬沙坦对急性心肌梗死患者左室结构和功能的影响

目的　观察缬沙坦对急性心肌梗死 (AMI)患者左室结构和功能的影响。方法　将 72例AMI患者随机分为两组 ,常规治疗组 36例 ,口服消心痛 10mg,3次 d;缬沙坦组 36例 ,口服消心痛 10mg ,3次 d ,缬沙坦80mg ,1次 d,并于治疗后 2周、 2 4周分别进行超声心动图和平衡法核素心室造影 ,测定左室心肌重量、左室收缩和舒张功能 ,了解缬沙坦对AMI后左室重塑的阻抑作用。结果　AMI治疗 2 4周时两组患者的室间隔厚度 (IVST)、左室后壁厚度 (LVPWT)、左室舒张末内径 (LVDd)和左室心肌重量指数 (LVMI)、左室射血分数 (LVEF)、左室峰射血率时间 (LPER)、左室高峰充盈率 (LPFR)和左室高峰充盈率时间 (LTPFR)的各均值间差别均有显著性意义 (P<0 0 5 )。结论　缬沙坦能明显减轻心肌梗死后心肌肥厚和左室重塑 ,改善左室功能。     

Differentiation plasticity of human fetal articular chondrocytes.

OBJECTIVE: To test chondrogenic differentiation potential, we examined the differentiation plasticity of isolated human fetal articular chondrocytes (HFACs). STUDY AND DESIGN SETTING: Culture-expanded human fetal articular chondrocytes (HFACs) were analyzed for chondrogenic, adipogenic, osteogenic capacity and neural differentiation ability in defined in vitro culture systems. RESULTS: The different assays demonstrated that culture-expanded HFACs have potential to form cartilage in pellet mass culture, to form adipose cells, osteogenic cells, and neural cells in monolayer culture. CONCLUSIONS: These results suggest that within human fetal articular cartilages there are MSC-like cells that exhibit differentiation plasticity that is comparable with that of BM-MSCs and they may be a new kind of seeding cells for head and neck cartilage reconstruction.     

体表电位标测微机系统(Ⅰ型)

本系统的107个电极中,103个分布于胸、背部,1个为参考电极,1个为V_3,其余用于肢导。本系统除能绘出2、4、8ms任意间隔的103通道同步的体表等电位图,常规12导联和V_(7 9)心电图,胸、背部位的心电图外,还可记录R峰值等电位图,∑R,Q波等电位图,nQ30、40,极大、极小轨迹图等。     

健康教育与大众媒体互动合作机制的定性研究

目的了解大众媒体报道艾滋病的现状，探索大众媒体与健康教育机构之间的互动合作模式。方法以艾滋病的宣传报道为切入点，对15名从事艾滋病宣传报道的负责人或记者进行个人深入访谈。结果影响大众媒体与健康教育机构之间合作的因素主要是资金、观念与机制。大众媒体与健康教育机构之间合作的最佳方式是相互理解、优势互补，建立健全双方长期、定时的信息交流机制和良好的互动合作机制，前者提供宣传平台，后者提供健康信息。结论大众媒体与健康教育机构之阃的合作既有基础，又有必要。加强两者的合作，将是一项双赢的策略。     

Single-sperm typing: determination of genetic distance between the G gamma-globin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers.

The frequency of recombination between the G gamma-globin (HBG2) and parathyroid hormone (PTH) loci on the short arm of human chromosome 11 was estimated by typing greater than 700 single-sperm samples from two males. The sperm-typing technique employed involves the polymerase chain reaction and allele-specific oligonucleotide hybridization. Our maximum likelihood recombination fraction estimate of 0.16 (95%) confidence interval, 0.13-0.19) falls well within previous estimates based on family studies. With current technology and a sample size of 1000 sperm, recombination fractions down to approximately 0.009 can be estimated with statistical reliability; with a sample size of 5000 sperm, this value drops to about 0.004. Reasonable technological improvements could result in the detection of recombination frequencies less than 0.001.     

使用自制Ralph刀切光镜用的高质量半薄切片及染色

在1981年,我们设计了BD—8102组织切片刀制刀机。该机能制出宽为25mm及38mm的高质量玻璃刀(Ralph)。借助一个简单刀架把Ralph刀装配到普通切片机上,就可切出用塑料或石蜡包埋的组织大切片。用这种方法可得到光镜用的高质量半薄切片。并报告近几年来用该刀切片和染片技术的体会。     

电子文献数据库收录期刊的质量瑕疵及对策

 

以限题检索为手段，以收录期刊所载论文为指标，比较分析了国内常用的4种医学电子文献数据库收录医学期刊的质量。结果表明4种医学电子文献数据库均存在收录“非法医学期刊”之瑕疵，但各数据库的收录量有差异。提示广大读者和电子文献数据库使用者注意，并针对此问题提出了相应的对策和建议。

     

Preliminary study on the nucleic acid hybridization technic in detecting HSV-2 DNA corresponding sequence of cancer tissues of the uterine cervix

In this paper, 34 samples, 25 from 18 cervical carcinoma patients and 9 from 8 non-cervical carcinoma patients, were assayed using (alpha-32P) dCTP HSV-2 DNA probe and nucleic acid hybridization technic. It was shown that the positive rates of HSV-2 DNA corresponding sequence were 50% (2/4), 36% (5/14) and 12% (1/8) in the in situ cancer, invasive cancer and non-cervical cancer tissues. No HSV was isolated from 7 cancer specimens which showed a positive reaction by nucleic acid hybridization.