The Influence of Facilitator and Facilitation Characteristics on Participants' Ratings of Stepfamily Education

We examine the relative importance of facilitator and facilitation characteristics on participant ratings of a stepfamily education program. Data from 48 facilitators and 598 participants suggest that quality facilitation is more meaningful to participants than whether facilitators have comparable demographic characteristics or life experiences. Hierarchical regressions identified time management effective use of personal experiences, and clear explanation of material as key facilitation skills. Popular assumptions about participant‐facilitator similarity and implications for programming are discussed.     

The Link Between Perceptions of Self and of Social Relationships in High-Functioning Children with Autism

This study examined the perception of friendship in high-functioning children with autism (8–17 years old) and the link between perceptions of self and of social relationships in these children. Sixteen typically developing children were matched to sixteen high-functioning children with autism, on chronological age, IQ, gender, and mother's education. Study measures included a friendship picture recognition task and three self-report questionnaires: qualities of friendship, loneliness, and self-perception profile. Main results indicated that even if children with autism more frequently related to the intersubjective qualities of friendship such as affective sharing or intimacy, they perceived their friendship to be as close as did typically developing children. Also, for the group with autism, friendship correlated positively with cognitive competencies and general self-worth and negatively with loneliness. In addition, children with autism perceived their social and athletic competencies as lower compared with typically developing children. Implications of the associations between self-perceptions and perceptions of friendship are discussed.     

Identification of quantitative trait loci for chemical/inflammatory nociception in mice

Sensitivity to pain is widely variable, and much of this variability is genetic in origin. The specific genes responsible have begun to be identified, but only for thermal nociception. In order to facilitate the identification of polymorphic, pain-related genes with more clinical relevance, we performed quantitative trait locus (QTL) mapping studies of the most common assay of inflammatory nociception, the formalin test. QTL mapping is a technique that exploits naturally occurring variability among inbred strains for the identification of genomic locations containing genes contributing to that variability. An F2 intercross was constructed using inbred A/J and C57BL/6J mice as progenitors, strains previously shown to display resistance and sensitivity, respectively, to formalin-induced nociception. Following phenotypic testing (5% formalin, 25 microl intraplantar injection), mice were genotyped at 90 microsatellite markers spanning the genome. We provide evidence for two statistically significant formalin test QTLs - chromosomal regions whose inheritance is associated with trait variability - on distal mouse chromosomes 9 and 10. Identification of the genes underlying these QTLs may illuminate the basis of individual differences in inflammatory pain, and lead to novel analgesic treatment strategies.     

Nonradiolabeling assay for WaaP,an essential sugar kinase involved in biosynthesis of core lipopolysaccharide of Pseudomonas aeruginosa

waaP is present in the lipopolysaccharide (LPS) core gene clusters of a wide range of gram-negative bacteria, and is an essential gene in Pseudomonas aeruginosa. The WaaP protein is a sugar kinase that adds phosphate to heptose I in the core oligosaccharide. This study describes the standardization and utility of a chemiluminescence-based enzyme-linked immunosorbent assay for the detection of WaaP kinase activity. Important features of the assay include high sensitivity, the preparation of dephosphorylated LPS as a substrate, and the use of monoclonal antibody 7-4 that specifically recognizes phosphate substituents in the LPS core.     

An approach to the three-dimensional display of left ventricular function and viability using MRI

Cardiac MRI was performed in human volunteers to determine the magnitude of the misregistration (MSR) of cardiac landmarks due to variability in the diaphragm position for repeated breath-holds. Seven normal volunteers underwent MR imaging of the left ventricle (LV) to evaluate the magnitude of the endocardial centroid MSR. The MSR for a mid-ventricle short-axis image was 3.01 ± 1.68 mm through-plane and 4.16 ± 1.62 mm in-plane. A second order polynomial fit through the LV centroid coordinates minimized the in-plane component of the MSR error. Short-axis cine images, corrected for MSR, provided high-resolution 2D data from which an accurate anatomical model of the LV was generated. Anatomical landmarks were used to register parametric maps of myocardial perfusion and viability to the three-dimensional (3D) model, with the corresponding parameters displayed as color-encoded values on the endo- and epicardial surfaces of the LV. Registration of regional wall motion, perfusion and viability to the 3D model was performed for three patients with a history of cardiovascular disease. The proposed 3D reconstruction technique allows visualization in 3D of the LV anatomy, in combination with parametric mapping of its functional status.