异基因造血干细胞移植后并发慢性移植物抗宿主病患者生活质量变化及其影响因素分析

目的探究异基因造血干细胞移植(allo-HSCT)后并发慢性移植物抗宿主病(cGVHD)患者生活质量及其影响因素。 方法选取2016年6月至2020年6月于浙江大学医学院附属第一医院骨髓移植中心行allo-HSCT并在移植后诊断为cGVHD的患者作为研究对象,研究时间为allo-HSCT后初次诊断cGVHD至确诊后9个月。收集allo-HSCT后并发cGVHD患者确诊cGVHD时以及确诊后3、6和9个月临床资料,并采用第4版癌症治疗功能评价-骨髓移植测评量表(FACT-BMT V4.0)对其进行问卷调查。非正态分布计量资料采用Kruskal-Wallis H检验进行比较。分类变量采用卡方检验进行比较。对allo-HSCT并发cGVHD患者确诊后3个月生活质量影响因素进行单因素分析,选取单因素分析中P<0.05的因素及患者年龄进行多元线性回归分析。P<0.05为差异有统计学意义。 结果最终纳入247例allo-HSCT后并发cGVHD患者,其中111例为轻度cGVHD,104例为中度cGVHD,32例为重度cGVHD,三组患者原发病及移植时间构成比例差异均有统计学意义(χ²＝15.446和44.456,P均<0.05)。轻、中、重度cGVHD组患者躯体状况、社会/家庭状况、情感状况、功能状况、干细胞移植模块得分以及FACT-BMT V4.0总分差异均有统计学意义(H＝41.184、16.277、22.695、27.014、60.112和64.645,P均<0.05)。截至2021年6月,247例allo-HSCT后并发cGVHD患者中位随访时间为948(246,1 867)d,其中68.4%(169/247)患者经系统治疗后获得完全缓解(CR)/部分缓解(PR)。获得CR/PR患者确诊cGVHD时以及确诊后3、6、9个月FACT-BMT V4.0总分分别为152(111,183)、124(95,154)、129(100,157)和152(120,182)分,各时间点差异有统计学意义(H＝344.113,P<0.05)。单因素及多元线性回归分析结果显示,cGVHD严重程度及糖皮质激素耐药是影响allo-HSCT后并发cGVHD患者确诊后3个月生活质量独立危险因素(P均<0.05)。 结论FACT-BMT V4.0在allo-HSCT人群中具有较好的实践价值。cGVHD严重影响allo-HSCT后患者生活质量,且与cGVHD严重程度及糖皮质激素耐药密切相关。经治疗后获得CR/PR者FACT-BMT总分呈动态上升趋势。     

腹腔镜下腹腔内补片修补术治疗腹壁切口疝的临床研究

目的探讨腹腔镜下腹腔内补片修补术（IPOM）治疗腹壁切口疝的安全性及可行性。 方法回顾性分析2014-2019年中国人民解放军南部战区总医院对103例腹壁切口疝患者实施腹腔镜下IPOM的临床资料,观察其手术时间、术后排气时间、术后住院时间、并发症及复发情况。 结果103例患者均顺利完成手术,平均手术时间、术后排气时间及术后住院时间分别为（100±10）min、（40±8）h及（7.5±1.5）d。术中发现1例合并脐疝同时行脐疝修补,1例合并右侧腹股沟直疝同时行TAPP术,1例嵌顿性肠梗阻并肠坏死同时行肠切除吻合术。术后轻微并发症经短期治疗后痊愈,随访3~24个月,均无切口疝复发。 结论腹腔镜下IPOM治疗腹壁切口疝具有安全、有效、创伤小、可发现隐匿疝、术后康复快、能降低并发症、缩短住院时间、复发率低等优点。     

MasonⅠ型及Ⅱ型桡骨头骨折保守治疗失败病例分析

目的分析经过保守治疗的Mason Ⅰ型及Ⅱ型桡骨头骨折病例失败的主要因素,同时探讨手术对保守治疗失败病例的疗效。 方法回顾性研究2010年2月至2015年6月在本中心行保守治疗的16~65岁急性Mason Ⅰ型及Ⅱ型的桡骨头骨折病例,排除合并同侧上肢其他损伤、颅脑外伤等影响桡骨头骨折预后的患者。收录信息包括患者一般情况、诊断、制动方式及时间,随访内容包括转为手术率、视觉模拟评分（visual analogue scale, VAS）、肘关节活动度、Mayo肘关节功能评分系统（Mayo elbow performance score, MEPS）等。 结果共612例患者符合纳入标准,其中成功随访患者345例,包括男205例、女140例,平均年龄37.5岁（17~65岁）,平均随访时间13.4个月（5~23个月）。345例患者中有27例（7.8%）患者因疼痛、活动障碍等原因转为手术治疗,转为手术治疗的患者平均制动时间要显著高于保守治疗成功组的患者,其中Mason Ⅱ型19例（70.4%）,3例（11.1%）术中证实存在骨软骨游离体,1例（3.7%）存在环状韧带关节囊嵌顿,4例（14.8%）存在外侧韧带复合体损伤,25例（92.6%）有明确的关节僵硬而行松解术,另有5例（18.5%）因康复过程中出现尺神经损伤而行神经探查松解术。27例转为手术的患者,平均VAS评分从术前（5.6±1.4）分降到术后（1.2±0.8）分,MEPS评分从（55.6±8.6）分提高到（89.6±4.9）分,平均上肢功能评分表（disability of arm shoulder and hand, DASH）从术前（44.4±9.8）分降至术后（15.4±5.9）分。 结论骨折类型、制动时间过长以及遗漏合并损伤是Mason Ⅰ型及部分Mason Ⅱ型桡骨头骨折保守治疗失败的主要原因,手术治疗可以满意解决相应的关节僵硬、疼痛、韧带和游离体嵌顿等并发症。     

Ciproxifan,an H3 receptor antagonist,improves short-term recognition memory impaired by isoflurane anesthesia

Background

Exposure to volatile anesthetics has been reported to cause temporary or sustained impairments in learning and memory in pre-clinical studies. The selective antagonists of the histamine H3 receptors (H3R) are considered to be a promising group of novel therapeutic agents for the treatment of cognitive disorders. The aim of this study was to evaluate the effect of H3R antagonist ciproxifan on isoflurane-induced deficits in an object recognition task.

Methods

Adult C57BL/6 J mice were exposed to isoflurane (1.3 %) or vehicle gas for 2 h. The object recognition tests were carried at 24 h or 7 days after exposure to anesthesia to exploit the tendency of mice to prefer exploring novel objects in an environment when a familiar object is also present. During the training phase, two identical objects were placed in two defined sites of the chamber. During the test phase, performed 1 or 24 h after the training phase, one of the objects was replaced by a new object with a different shape. The time spent exploring each object was recorded.

Results

A robust deficit in object recognition memory occurred 1 day after exposure to isoflurane anesthesia. Isoflurane-treated mice spent significantly less time exploring a novel object at 1 h but not at 24 h after the training phase. The deficit in short-term memory was reversed by the administration of ciproxifan 30 min before behavioral training.

Conclusion

Isoflurane exposure induces reversible deficits in object recognition memory. Ciproxifan appears to be a potential therapeutic agent for improving post-anesthesia cognitive memory performance.

     

Elevated plasma aldosterone is an independent risk factor for erectile dysfunction in men

Introduction

Erectile dysfunction (ED) and cardiovascular disease (CVD) share a great number of common risk factors. There is growing evidence that aldosterone, an independent CVD risk factor, is associated with ED.

Aims

The purpose of this study was to determine the relationship between plasma aldosterone and erectile dysfunction.

Methods

This study recruited 287 participants, ranging from 18 to 84 years old; 217 were suffering from ED, diagnosed by the International Index of Erectile Function 5 (IIEF-5) scores. Based on IIEF-5 scores, patients were divided into one control group and three ED groups (mild ED; moderate ED; severe ED).

Main outcome measures

The differences in principal characteristics, blood routine, sexual hormone, adrenal hormone, thyroid hormone, renal function, liver function and blood lipid were compared between ED and control groups.

Results

Our study demonstrated that the difference of mean plasma aldosterone levels between ED group and the control group was statistically significant (P < 0.05). Stepwise logistic regression analysis of all the possible factors support the role of aldosterone as an independent risk factor for ED (OR 1.011; 95 % CI 1.003–1.018; P = 0.004). Similar statistical methods were applied to the comparison between moderate to severe ED group and control to mild ED group (OR 1.017; 95 % CI 1.009–1.024; P < 0.001). ROC curve and the area under the curve (0.718; 95 % CI 0.643–0.794; P < 0.001) were performed to assess the diagnostic effect and to compare the severity of risk with the known independent risk factors, such as age and cholesterol (0.704; 95 % CI 0.631–0.778; P < 0.001). When using a 374 pg/mL cut-off value from Youden index, the OR of ED group versus controls is 3.106 (95 % CI 1.458–6.617), while the OR of moderate to severe ED versus control and mild ED is 5.480 (95 % CI 3.108–9.662).

Conclusions

We determined that elevated plasma aldosterone concentration is an independent risk factor for ED. Our findings also indicate that the aldosterone, a well-recognized contributor to vascular injury, might be a potential bond between ED and CVD.