全文获取类型
收费全文 | 17833篇 |
免费 | 1285篇 |
国内免费 | 67篇 |
专业分类
耳鼻咽喉 | 127篇 |
儿科学 | 547篇 |
妇产科学 | 538篇 |
基础医学 | 2476篇 |
口腔科学 | 291篇 |
临床医学 | 2034篇 |
内科学 | 3368篇 |
皮肤病学 | 478篇 |
神经病学 | 1798篇 |
特种医学 | 492篇 |
外科学 | 1875篇 |
综合类 | 113篇 |
一般理论 | 7篇 |
预防医学 | 1951篇 |
眼科学 | 423篇 |
药学 | 1055篇 |
1篇 | |
中国医学 | 19篇 |
肿瘤学 | 1592篇 |
出版年
2024年 | 25篇 |
2023年 | 234篇 |
2022年 | 355篇 |
2021年 | 686篇 |
2020年 | 465篇 |
2019年 | 583篇 |
2018年 | 757篇 |
2017年 | 509篇 |
2016年 | 604篇 |
2015年 | 669篇 |
2014年 | 824篇 |
2013年 | 1109篇 |
2012年 | 1631篇 |
2011年 | 1600篇 |
2010年 | 811篇 |
2009年 | 759篇 |
2008年 | 1163篇 |
2007年 | 1175篇 |
2006年 | 1026篇 |
2005年 | 948篇 |
2004年 | 825篇 |
2003年 | 752篇 |
2002年 | 713篇 |
2001年 | 86篇 |
2000年 | 50篇 |
1999年 | 76篇 |
1998年 | 106篇 |
1997年 | 101篇 |
1996年 | 62篇 |
1995年 | 52篇 |
1994年 | 49篇 |
1993年 | 56篇 |
1992年 | 39篇 |
1991年 | 24篇 |
1990年 | 21篇 |
1989年 | 19篇 |
1988年 | 7篇 |
1987年 | 8篇 |
1986年 | 18篇 |
1985年 | 16篇 |
1984年 | 18篇 |
1983年 | 16篇 |
1982年 | 23篇 |
1981年 | 12篇 |
1980年 | 10篇 |
1979年 | 8篇 |
1978年 | 10篇 |
1977年 | 9篇 |
1971年 | 8篇 |
1967年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
41.
Shields CM Taylor R Nazarenus T Cheatle J Hou A Tapprich A Haifley A Atkin AL 《Current genetics》2003,44(4):184-194
Saccharomyces cerevisiae ATS1 (-tubulin suppressor 1) was originally identified as a high-copy suppressor of class two -tubulin mutations and was proposed to have a regulatory role in coordinating the microtubule state with the cell cycle. Here, we show that Ats1p interacts with Nap1p, a cytoplasmic protein that regulates the activity of the Cdc28p/Clb2p complex. Loss of Nap1p results in a delayed switch from polar to isotropic bud growth. The delayed switch results in elongated buds. Nap1p and Ats1p interact in two-hybrid and co-immunoprecipitation assays. Both nap1 and ats1 cells have a Clb2p-dependent elongated bud morphology. Deletion of ATS1 partially suppresses the elongated bud morphology and benomyl resistance of nap1 mutants. Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p.Communicated by S. Hohmann 相似文献
42.
43.
44.
Infantile hemangioma is a proliferation of beta 4-negative endothelial cells adjacent to HLA-DR-positive cells with dendritic cell morphology 总被引:4,自引:0,他引:4
Although hemangioma is referred as to the most common tumor in infancy, the underlying pathogenetic events and the biologic origin of this benign vascular neoplasm have remained obscure. By using immunohistochemistry on frozen sections of infantile hemangiomas, we show here that proliferating endothelial cells abundantly expressed alpha(v)beta(3) but lacked beta(4) integrins. Instead, regressing and involuting infantile hemangiomas due to treatment with IFN-alpha showed positive staining of beta(4) integrin, which might point to the angiogenic significance of beta(4) integrin in infantile hemangiomas. Moreover, immunofluorescence analysis revealed the existence of HLA-DR(+), mostly CD68(+) and partly DC-SIGN/CD209(+) cells with dendritic cell morphology in the intimate vicinity of hemangiomatous vessels. Such cells were also detected in the dermal microvascular unit in normal skin. The coupled occurrence of vascular structures and perivascular cells that were stained positive with markers of monocyte or macrophage or dendritic cells might suggest that the development of infantile hemangioma is a result of vasculogenesis, that is, the formation of primitive blood vessels from angioblasts, rather than of angiogenesis, that is, the sprouting of capillaries from preexisting vessels. 相似文献
45.
46.
Beth Gordesky-Gold John M. Warrick David P. Kutzler Karama C. Neal Christina M. Coughlin Laurie Tompkins 《Behavior genetics》1996,26(1):49-54
Larvae from seven laboratory strains and eight isofemale lines ofDrosophila melanogaster differ significantly with regard to their responses to light in a photokinesis assay in which the larvae are tested en masse.
Larvae from the CA-2 laboratorystock fail to disperse on assay plates, although observations of individual CA-2 larvae suggest
that the larvae are repelled by light. Larvae from all of the other laboratory stocks and all of the isofemale lines (except
LI2 and NC5) avoid light in the photokinesis assay. Larvae from some stocks are much more strongly repelled by light than
larvae from other stocks. LI2 larvae are unresponsive to light in most replicates of the photokinesis assay, while NC5 larvae
are consistently unresponsive to light. Observations of F1 heterozygotes suggest that the allele(s) that affects the vision of LI2 and NC5 larvae has net effects on the animals' behavior
that are partially dominant and recessive, respectively. 相似文献
47.
Fritz Thorey Christina Stukenborg-Colsman Henning Windhagen Carl Joachim Wirth 《Technology and health care》2008,16(2):85-92
Today the use of pneumatic tourniquet is commonly accepted in total knee arthroplasty (TKA) to reduce perioperative blood loss. There are a few prospective randomised and nonrandomised studies that compare the effect of tourniquet release timing in cementless or cemented unilateral TKA. However, many of these studies show an inadequate reporting and methodology. This randomized prospective study was designed to investigate the efficiency of tourniquet release timing in preventing perioperative blood loss in a simultaneous bilateral TKA study design. To our knowledge, this is the first study of its kind, in which the effect of tourniquet release timing on perioperative blood loss was investigated in simultaneous bilateral cemented TKA to compare both techniques intraindividually. In 20 patients (40 knees) one knee was operated with tourniquet release and hemostasis before wound closure, and the other knee with tourniquet release after wound closure and pressure dressing. We found no significant difference in total blood loss between both techniques (p=0.930), but a significant difference in operating time (p=0.035). There were no postoperative complications at a follow-up of 6 month. Other studies report an increase the blood loss in early tourniquet release and an increase the risk of early postoperative complications in deflation of tourniquet after wound closure. In this study we found no significant difference in perioperative blood loss and no increase of postoperative complications. Therefore, we recommend a tourniquet release after wound closure to reduce the duration of TKA procedure and to avoid possible risks of extended anaesthesia. 相似文献
48.
Krister W. Fjermestad Rene Huster Christina Thunberg Simen Stokke Claus H. Gravholt Anne‐Kristin Solbakk 《American journal of medical genetics. Part C, Seminars in medical genetics》2020,184(2):482-492
A few studies have examined neuropsychological functions, sleep, and mental health combined in Klinefelter syndrome (KS; 47,XXY). We investigated neuropsychological functions with standard tests, sleep with actigraphy, and self‐reported mental health in 30 men with KS (Mean age = 36.7 years) compared to 21 controls (Mean age = 36.8 years). Men with KS scored significantly lower on mental speed, attention span, working memory, inhibition, and set‐shifting tests, as well as overall IQ (mean effect size difference Cohen's d = 0.79). Men with KS had significantly longer night wakes, with no differences in other sleep variables (mean d = 0.34). Men with KS reported poorer mental health than controls (mean d = 1.16). Regression analyses showed neuropsychological functions explained variance in some sleep domains for men with KS but not for controls. Neuropsychological functions explained variance in some mental health domains for controls. For men with KS, however, verbal IQ was the only significant predictor of mental health. Altogether, men with KS display problems in neuropsychological functions and mental health but do not appear different from controls on most sleep parameters. Our findings indicate that relations between neuropsychological functions, sleep, and mental health differ between men with KS and controls. 相似文献
49.
50.
Christina Brahe Stefania Zappata Isabella Velon Enrico Bertini Serenella Servidei Pietro Tonali Giovanni Neri 《American journal of medical genetics. Part A》1993,45(3):408-411
Linkage analysis and prenatal prediction in families segregating autosomal recessive spinal muscular atrophy (SMA) has become feasible since the assignment of the locus responsible for type I-III SMA to region 5q12-q13.3. We have performed a segregation study of SMA in Italian families using molecular probes and highly informative PCR-based polymorphic markers. In one family, a 7-year-old boy affected with type III SMA and an 8-year-old apparently healthy brother had identical haplotypes. These findings prompted us to reexamine the apparently unaffected child. His neurological exam was normal. However, the electromyography (EMG) showed a pattern consistent with chronic SMA. To our knowledge this is the first example of presymptomatic diagnosis of SMA based on genotype analysis. © 1993 Wiley-Liss, Inc. 相似文献