An open-label,prospective, randomized,controlled, multicenter,phase 1b study of StrataGraft skin tissue versus autografting in patients with deep partial-thickness thermal burns

ObjectiveThis open-label, controlled, randomized study assessed the safety, tolerability, and efficacy of StrataGraft tissue compared to autograft in the treatment of deep partial-thickness (DPT) burns.MethodsThirty subjects with DPT thermal burns (3%–43% total body surface area) were treated with StrataGraft tissue as follows: cohort 1, ≤220 cm² refrigerated tissue; cohort 2, ≤440 cm² refrigerated tissue; and cohort 3, ≤440 cm² cryopreserved tissue. On each subject, two comparable areas of DPT burn were randomized to receive StrataGraft tissue or autograft. Coprimary end points were the percent area of the StrataGraft tissue treatment site undergoing salvage autografting by Day 28 and wound closure of treatment sites by 3 months.ResultsBy Day 28, no StrataGraft tissue treatment sites underwent autografting. By 3 months, 93% and 100% of the StrataGraft tissue and autograft treatment sites achieved complete wound closure, respectively. No significant differences in observer total and overall opinion POSAS scores between StrataGraft tissue and autograft treatment sites were observed at any timepoint. The most common adverse event was pruritus (17%).ConclusionsStrataGraft tissue treatment of DPT thermal burns reduced the need for autograft, resulted in wound closure and treatment-site cosmesis comparable to that of autograft, and was well tolerated.     

Use of a semi-automated object based analysis to map benthic composition,Heron Reef,Southern Great Barrier Reef

Coral reef scientists and managers require detailed information of reef environments to develop, implement and evaluate management strategies. Maps showing the distribution of corals, seagrass, algae, sand, rubble and rock are an essential component of this information. Using a semi-automated, object-based image analysis (OBIA) approach this study created a benthic community map for Heron Reef (area: approximately 27 km²), Southern Great Barrier Reef, Australia. Benthic cover information was acquired for calibration and validation via georeferenced photograph transects, which coincided with high spatial resolution Quickbird-2 satellite image capture in 2007. Through combination of these data sets and modification of a semi-automated OBIA classification scheme previously developed for seagrass environments to account for the heterogeneous nature of reef benthic communities, a benthic community map was created for Heron Reef with an overall accuracy of 61.6%. The robustness and repeatability of our method provide future opportunity to apply the semi-automated approach to a time series of field and high spatial resolution image data sets to create a time series of benthic community maps for Heron Reef. The results presented in this study therefore open up the opportunity to assess and understand the spatial and temporal dynamics of the reef environment.