MR动态增强扫描在前列腺癌诊断及鉴别诊断中的应用价值(英文)

目的通过绘制时间信号强度曲线,探讨 MRI 快速序列动态增强在前列腺癌诊断及鉴别诊断中的价值。方法对5例无泌尿系症状健康对照者、13例经病理证实的前列腺癌及36例前列腺增生行 MR 平扫、动态增强及延迟扫描,测量并计算病灶和正常组织的相对信号强度值,并绘制正常周围带、前列腺癌与增生三者的时间信号强度曲线。结果正常周围带轻度强化,并缓慢上升至晚期达峰值;36例前列腺增生早期明显强化并逐渐上升至中晚期达峰值后缓慢下降;13例前列腺癌中9例早期明显强化,并快速下降,4例 T_2WI 像上弥漫性病灶呈现中晚期强化。结论正常周围带、前列腺癌及前列腺增生的动态强化方式明显不同,应用动态增强扫描可对前列腺癌的诊断与鉴别诊断起积极作用。     

婴儿双歧杆菌/胞嘧啶脱氨酶肿瘤靶向性基因治疗系统的构建(英文)

目的构建婴儿双歧杆菌/胞嘧啶脱氨酶靶向性基因治疗系统。方法 PCR 扩增 CD 基因,EcoR Ⅰ,BamH Ⅰ对 CD 基因和 pGEX-1LamdaT 质粒同时进行双酶切,获得4.9 kb、1.3 kh 两个 DNA 片段。T4 DNA 连接酶连接这两个片段构建重组的CD/pGEX-1LamdaT 质粒,然后用电穿孔法将重组质粒转染婴儿双歧杆菌。从阳性转染的婴儿双歧杆菌中提取重组质粒,双酶切后检测切取片段长度,采用 Sanger 双脱氧链终止法对提取的重组质粒中的插入片段进行测序。结果从阳性转染的婴儿双歧杆菌中获得了6.2 kb大小的重组质粒,该质粒经双酶切后,得到了4.9 kb 和1.3 kb 两个长度的片段,其长度分别与 pGEX-1LambdaT 及 CD 基因的长度相同。测序结果显示,提取的重组质粒中插入的基因片段全长及核苷酸序列与 CD 基因完全相同。结论外源性 CD 基因被准确插入 pGEX-1LambdaT 质粒并转入婴儿双歧杆菌中,婴儿双歧杆菌/胞嘧啶脱氨酶靶向性基因治疗系统被成功构建。     

肺癌神经内分泌分化与术后生存关系探讨(英文)

目的探讨非小细胞肺癌神经内分泌(NSCLC-NE)分化与患者手术后生存关系。方法收集1997年4月～1999年4月98例肺癌手术切除病理标本,采用免疫组化标记特异性烯醇化酶(NSE)及突触素(SY),并按强弱区分为“+、++、+++”。对同一手术病例标本采用电镜观察特异性 NE 颗粒。术后病例随访36例,最长60月。采用Cox 多因素风险模型分析 NSCLC-NE 分化与患者术后生存的关系。结果 91例为非小细胞肺癌。非小细胞肺癌 NE 阳性表达率为63.7%(58/91),其中 NSE 阳性表达54例(59.3%),SY 阳性表达22例(24.1%),电镜观察 NE 特异性颗粒30例(33.0%)。结合免疫组化和电镜观察 NSCLC-NE 分化44例(48.4%)。Cox 模型多因素分析结果表明 NSCLC-NE 分化者术后生存时间明显缩短(P=0.048)。术后生存与肺癌细胞分化程度(P=0.006)、病理分期(P=0.001)、NE 表达强弱(P=0.054)有密切关系。结论 NSCLC-NE 分化与肿瘤细胞分化和患者术后生存有关。采用 NE 标志特标记肿瘤,并观察其强弱改变,对术后评估具有较重要的参考意义,可作为临床判断患者预后指标之一。     

经腹根治性宫颈切除术4例临床体会

目的探讨可保留生育功能的经腹根治性宫颈切除术的临床疗效。方法为4例希望保留子宫功能的宫颈病变患者实施经腹根治性宫颈切除术。结果4例患者术后均恢复月经,术后随诊情况良好。结论经腹根治性宫颈切除术可以保留宫颈病变患者子宫的功能,但其生存率是否与传统方法相近,需进一步临床观察研究。     

EFFECT OF ELECTROACUPUNCTURE AND CALCIUM-CHANNEL INHIBITORS ON CYTOPLASMIC FREE CALCIUM CONCENTRATION OF MOUSE BRAIN CELLS

Ithasbeenrecognizedthattheunivalentandbi valentionsareimportanttothenormalactivitiesof thenervoussystem,especially,Ca2+playsakeyrole inthestabilityandpermeabilityofneurilemma,the transmissionofnerveimpulses,releaseoftransmitters ofthenerveendingsandtheirresultantbiologicef fectsthroughthepost synapticmembrane,generation andaftereffectsofthesecondmessenger,etc.[1,4].Manyresearcheshaveshowedthattheanalgesiceffect ofmorphine likedrugsandendogenousmorphine like substances(endorphin,etc)isrealizedb…     

CLINICAL OBSERVATION ON 40 CASES OF ANKYLOSING SPONDYLITIS TREATED WITH ACUPUNCTURE AND CHINESE DRUGS

Ankylosing spondylitis(AS)is a systemic dis-ease marked by chronicinflammation of the sacro-iliacjoint and the spinal column.Its pathologic changesare inflammation of the attachment point of the mus-cle tendons and ligaments,rigidity,deformity andeven severe dysfunction of the spineinthelate stage.This disease is mostly seenin young and middle-agedpeople,possesses an obviousfamilial disposition andisclosely related to human leukocyte antigen(HLA)-B27.But its pathogenic causes remain unknown …     

CLINICAL OBSERVATION ON THE TREATMENT OF CHRONIC FATIGUE SYNDROME WITH DEEPLY PUNCTURED ELONGATED NEEDLE COMBINED WITH COMPREHENSIVE NURSING

Chronicfatiguesyndrome(CFS)referstoa groupofclinicalsymptomsandsignsincludingmain symptom extremefatiguewithoutapparentreason,andconcurrentsymptomsandsignsaspoormemory,inabilitytoconcentrate,sorethroat,tendernessof thelymphnodesaroundtheneckorinthearmpitre gion,lowfever,headache,muscleandjointache,somnipathy,andnoticeablepost exertionalmalaise whicheitherhangonorcomeandgofrequentlyfor morethan6months.Inaddition,inpeoplewith CFS,physicalexaminationandroutinelabtestshow noanyabnormalfindings.I…     

TREATMENT OF CEREBRAL PALSY WITH APHASIA BY LINGUISTIC TRAINING COMBINED WITH ACUPUNCTURE

Aphasiaisaconcurrentdisorderofcerebralpalsy(CP)childrenanditsincidencerateisbout70%-75%[1].Itoftenbringsthepatientwithseveredisor dersindaily lifeself careanddirectlyhindsthechild patient’scognitivedevelopment,learningandsocial intercourse.Thus,therehabilitationtreatmentofCP children’saphasiaisveryimportant.FromMay2000toDecember2001,theauthorsofthepresentpaper observedthetherapeuticeffectof“consciousness restoringneedling”pluslinguistictraininginCPchil dren.Followingisthereport.1CLIN…     

"APONEUROTIC SYSTEM" IN MEDICAL CLASSIC THE YELLOW EMPEROR''''S CANON OF INTERN AL MEDICINE IS THE EARLIEST DESCRIPTION ON NERVOUS SYSTEM IN HUMAN HI STORY

IntraditionalChinesemedicine(TCM),the channel(meridian)collateralsystemisclassifiedas channel,collateral,“Jingjin”(经筋aponeuroticsys temormusclesortendinomuscularstructuresalong thetwelveregularchannels)anddermalpart,etc.;accordingtothedifferentdistributionofthesystemin thehumanbody,itisspecificallydividedintotwelve regularchannels,twelvebranchesofregularmeridi ans,eightextrachannels,collaterals,fifteenmajor collaterals,minutechannels,minutecollaterals,su perficialcollaterals,“Jingjin”,a…     

慢性乙型肝炎病毒感染者血清白细胞介素-2干扰素-γ检测及其临床意义

目的探讨慢性乙型肝炎病毒(HBV)感染者外周血白细胞介素-2(IL-2)、干扰素(IFN)-γ活性与病毒载量、肝功能以及肝纤维化的关系。方法IL-2检测采用放射免疫法(RIA法),IFN-γ检测采用酶联免疫吸附试验(ELISA)。结果HBV-DNA高载量组与低载量组相比IL-2水平明显低下(P<0.05),两组间IFN-γ水平无统计学意义。随着肝功能损害程度加重,IL-2水平逐渐降低,IFN-γ逐渐增高;与非肝硬化组比较,肝硬化组的IL-2的含量显著降低,而IFN-γ显著升高(P均<0.01)。结论在乙型肝炎慢性化过程中,IL-2与HBV清除有关,其降低的程度与肝损伤严重程度及肝纤维化相关;IFN-γ的抗病毒作用不明显,但它作为炎症介质参与了肝脏炎症坏死和肝纤维化的形成过程。