穿心莲内酯对TBHP诱导的椎间盘髓核细胞凋亡的作用及机制研究

目的：探讨穿心莲内酯（andrographolide,ANDRO）对叔丁基过氧化氢（tert-butyl hydroperoxide,TBHP）诱导的椎间盘髓核细胞（nucleus pulposus cells,NPC）凋亡的作用及其机制。方法：用TBHP诱导NPC建立细胞模型。将NPC分为5组：对照组、模型组（100μmol/L TBHP）、ANDRO低剂量组（100μmol/L TBHP+9μmol/L ANDRO）、ANDRO中剂量组（100μmol/L TBHP+18μmol/L ANDRO）和ANDRO高剂量组（100μmol/L TBHP+36μmol/L ANDRO）。流式细胞术检测细胞凋亡和活性氧（reactive oxygen species,ROS）水平,ELISA试剂盒检测氧化应激相关指标超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-PX）和过氧化氢酶（catalase,CAT）水平,JC-1探针法检测线粒体膜电位,Western blot检测动力相关蛋白1(dynam...     

区域神经阻滞复合甲苯磺酸瑞马唑仑在加速康复外科膝关节置换术中的应用1例

<正>全膝关节置换术(total knee arthroplasty,TKA)是减轻终末期膝关节骨性关节炎(osteoarthritis,OA)患者疼痛、恢复膝关节功能、提高生活质量最有效的治疗手段^[1-2]。过去膝关节置换术大多采用椎管内麻醉或气管插管全身麻醉,存在椎管内穿刺困难、反复穿刺导致组织损伤、术中血流动力学波动明显,术后呼吸功能障碍、苏醒延迟、拔管困难送重症监护室治疗的风险,且影响术后早期功能锻炼,     

鹿茸的FTIR性质与传统质量等级之关系

研究了鹿茸的傅里叶变换红外光谱(FTIR)特性与传统质量的关系。发现鹿茸质量等级的内在数字化“Z”值规律性。从而为鹿茸片特别是用目前方法难于确定质量等级的鹿茸粉,提供了质量等级的客观指标。     

Management of hemangiopericytomas in the central nervous system

目的　血管外皮细胞瘤是少见的中枢神经系统肿瘤 ,由于其易复发和转移而预后不良。本文介绍 2 6例血管外皮细胞瘤的处理经验并结合文献复习其诊治要点。方法　回顾性分析 1993年 1月至 1999年 6月华山医院收治的 2 6例血管外皮细胞瘤 ,其中 5例为复发肿瘤 ,1例为术后残留。结果　 2 6例中 2 4例病灶全切除 ,2例次全切除。术后 2 1例症状好转或不变 ,4例加重 ,1例手术后死于并发症。18例术后放疗。 18例获随访 ,随访期 1- 5 8个月 ,其中 15例恢复良好 ,2例发现转移肿瘤 ,1例复发再次手术后死亡。结论　手术全切是主要治疗方法 ,术后放疗可延缓肿瘤复发。     

Hepatic artery angiography and embolization for hemobilia after hepatobiliary surgery

目的　观察血管造影和肝动脉栓塞术对胆道术后胆道出血的诊断和治疗价值。方法　 9例在胆道手术后 7天到 3个月出现上消化道出血。所有患者行急诊肝动脉造影并采用明胶海绵和微钢圈进行肝动脉栓塞治疗。结果　肝动脉造影检查可见肝动脉假性动脉瘤 3例 ;肝动脉弥漫性出血 3例 ;右肝动脉胆道瘘 2例和肝动脉小肠瘘 1例。通过肝动脉栓塞控制胆道出血 7例 ,其中 1例再出血。 7例患者随访其中 3例死于多功能脏器衰竭。 2例因技术原因无法止血 ,数天后死亡。结论　胆道手术后出现上消化道出血如怀疑是胆道出血 ,应首先采用肝动脉血管造影检查 ,同时尽可能早地采用选择性肝动脉栓塞术治疗胆道出血 ,可获得较好的临床效果。     

Percutaneous balloon angioplasty of coarctation of the aorta in children: 12 -year follow-up results

Objective To evaluate the efficiency of percutaneous balloon angioplasty of coarctation o f the aorta in children and discuss its risk factors that can lead to poor long -term results.Methods From September 1987 to August 1999, 24 patients underwent 27 balloon angioplasty procedures for native or recurrent coarctation of the aorta at our institution . There were 19 patients with discrete (membranous) coarctation, 4 patients wit h long-segment and aortic arch hypoplasia, and 1 patient with postoperative recu rr ence. The balloon diameter was chosen not to exceed the diameter of the aorta p roximal to the stenotic site (11.06±2.80 mm vs 11.78±3.18 mm), with th e chosen diameter two to four times that of the stenotic segment (2.57±0.68 t imes). The patients have been followed up for half to twelve years (mean 6.2 ±2.8 years). Satisfactory result was defined as a reduction in the pressure g radient across the site of coarctation to ≤20 mm Hg. Results The systolic pressure gradient across the coarctation site decreased from 48. 17±14.68 mm Hg to 14.96±13.12 mm Hg (P&lt;0.01) and the diameter of the coarctation site increased from 4.66±2.43 mm to 8.80±3.32 mm (P&lt;0. 01). Immediate satisfactory results were obtained in 19 patients (79%). Of the 5 patients with unsatisfactory results, 4 had aortic arch hypoplasia and 1 had membranous coarctation. Of the 19 patients with satisfactory results, 18 patie nts had membranous coarctation and 1 patient had recurrent postoperative coarcta tion. No aneurysm and other complications had occurred in any patients at the follow-ups. Conclusion Percutaneous balloon angioplasty is an effective treatment alternative to surger y in most patients with recurrent postoperative or native membranous coarctation of the aorta. It should not be recommended for the coarctation of aortic arch hypoplasia.     

孕妇血清标记物在中孕筛查中的应用

目的 :通过对孕妇血清标记物甲胎蛋白 (AFP)和人绒毛膜促性腺激素 ( β -HCG )测定来筛选缺陷儿。 方法 :用放射免疫法对 6 2例孕 14～ 2 3周妊娠妇女上述标记物连测 3次 ,每次隔 12～ 14d ,以两次异常为高危个体。每例筛查者跟踪至胎儿出生后。结果 :在筛查中发现了 1例先天愚型和 1例无脑儿。结论 :上述两种血清标记物可以作为神经管缺陷和先天愚型筛查的一项常规方法     

急性冠状动脉综合征患者凝血因子Ⅶ促凝活性的测定及临床意义

目的探讨急性冠状动脉综合征(ACS)患者血浆凝血因子Ⅶ促凝活性(FⅦC)的临床意义.方法采用一期血凝法测定41例ACS患者和30例健康者血浆中凝血因子Ⅶ促凝活性.结果 ACS患者血浆凝血因子Ⅶ促凝活性(116.37±36.40)%明显高于健康者(73.55±15.73)%(P＜0.01).结论血浆凝血因子Ⅶ促凝活性增高、高凝状态的存在为ACS的发病基础,可以为临床上采用不同的治疗手段防治ACS提供一定的理论依据.     

An experimental study on nerve regeneration and spinal neurons after CO2 laser anastomosis of rat sciatic nerve

Objective: Many methods have been used in an attempt to seal the epineurium and to prevent axonal outgrowth.In this study, the rat sciatic nerves were repaired with CO2 laser, the nerve regeneration and the morphology of spinal anterior horn neurons were investigated. Methods: Seventy-two male Sprague-Dawley rats were randomly divided into 6 groups of 12 rats. The animals were designed to observe the electrophysiology, the histopathology and the morphology of spinal anterior horn neurons. One of the rat sciatic nerve anastomosed with CO2 laser, the contralateral nerve was reconstructed by microsuture technique. At 2, 4, 6, 8 weeks postoperatively, neuromuscular functions, the regeneration of axons and neurons were evaluated by the electro-physiological and histopathological studies. The rats were killed at 4, 6 weeks postoperatively. Results: The recovery of toe spread and myodynamia in laser groups was better than that in suture groups (P＜0.05). The latency of foot withdraw caused by radiate heat and neuromuscular conduction velocity in laser groups were faster than that in suture groups (P＜0.05). The density of nerve fibers, percentage of axons passing through anastomotic area and numbers of neurons were better in laser groups than in suture groups. At 8 weeks postoperatively, the first grade dendrites of anterior horn neurons grew well. Their diameter, length, volume and total volume were much higher than that in control group. (P＜0.05, P＜0.01). Conclusion: CO2 laser repairing was effective in promoting the regeneration and the recovery of sciatic nerves in its earlypost-trauma stage. In addition, laser repairing was found to reduce regenerating axons misdirection and forming neuroma.