运动训练对雄性大鼠垂体─性腺轴功能的影响

本研究通过使用自己制造的大鼠流动水游泳池，对雄性大鼠进行了６周递增负荷的游泳训练，以此来探讨运动训练对大鼠垂体－性腺轴功能的影响，结果显示：与安静对照大鼠相比，训练大鼠的一般状态较差，血清睾酮水平显著降低（Ｐ＜０．０５），血清睾酮／皮质酮比值平均降低了３０％以上；而血清ＬＨ和ＦＳＨ水平未见明显变化。通过对大鼠睾丸组织ＬＨ／ＣＧ受体的测定，显示运动训练虽未引起其最大结合量的改变，却能使其解离常数显著升高（Ｐ＜０．０５）。提示此种训练可能通过降低睾丸ＬＨ／ＣＧ受体对垂体分泌ＬＨ的亲和力，从而影响睾酮的合成。     

抗体阴性重症肌无力发病与凝集素之间关系研究

借助研究抗体阴性重症肌无力（ＭＧ）发病与凝集素之间的关系，以阐明其发病过程是否与凝集素有关。方法观察伴刀豆球蛋白Ａ（ＣｏｎＡ）和麦胚凝集素（Ｔｒｉｔｉｃｕｍ）及其凝集素－糖复合物对ＴＥ６７１细胞表达的乙酰胆碱受体（ＡＣｈＲ）功能的作用，以及对α－ＢｕＴｘ结合试验的影响。结果有两种凝集素对ＡＣｈＲ功能均有抑制作用，抑制率（％）分别为５４±１４（ｎ＝１１）和４７±１６（ｎ＝１０），此作用可被３种糖抑制，抑制率（％）分别为：９５±５（ｎ＝５）和８４±８（ｎ＝５）；６９±６（ｎ＝４）和６５±５（ｎ＝４）；３９±４（ｎ＝５）和５７±６（ｎ＝５）；ＣｏｎＡ抑制α－ＢｕＴｘ结合试验，而Ｔｒｉｔｉｃｕｍ则不能。结论Ｔｒｉｔｉｃｕｍ和抗体阴性ＭＧ患者非ＩｇＧ部分对ＡＣｈＲ功能和α－ＢｕＴｘ结合试验的作用类同或一致，表明抗体阴性ＭＧ患者非ＩｇＧ部分中的内源性Ｔｒｉｔｉｃｕｍ样糖蛋白在其发病过程中起重要作用。     

A case of recrudescent groove pancreatitis, which was in remission by proximal gastrectomy with vagotomy for gastric cancer]

This report describes our experience with a 60 year old male who suffered from a recrudescence of groove pancreatitis. He had been treated by conservative medication therapy by proton pump inhibitor used for therapy of duodenal ulcer, and was in remission. During a follow-up one year later, endoscopy revealed gastric cancer, for which a proximal gastrectomy and vagotomy were performed. The patient continues to remain in remission for the groove pancreatitis. Our experience with the clinical course of this disease, in which treatment for duodenal ulcer was used effectively, offers new insights into the progression and therapy of groove pancreatitis.     

热休克反应对大鼠油酸肺保护作用的机制研究

未研究发现，经热休克处理的大鼠注油酸后，肺组织损伤明显减轻，Ｗｅｓｔｅｒｎ印迹分析显示热休克使大鼠肺组织中ＨＳＰ７０含量明显增多；Ｎｏｒｔｈｅｒｎ印迹分析证实此乃由于ＨＳＰ７０ｍＲＮＡ转录增加所致。说明热休克处理使肺组织ＨＳＰ７０基因表达增强；提示热休克反应对大鼠油酸肺的保护作用与肺组织细胞中ＨＳＰ７０基因表达增强及与ＨＳＰ７０增多有关。     

Nucleotide changes around the splicing acceptor of intron 24 in the factor VIII gene and its impact on splicing.

Nucleotide 6724 of the factor VIII gene harbors a polymorphism of low frequency. A report from Taiwan claimed that 97.9% of the 83 alleles examined were of the A nucleotide at this position, which is quite different to the data from Western populations. Furthermore, this nucleotide is the start of exon 25, located in juxtaposition to the splicing acceptor of intron 24. We wonder if the nucleotide change at this location might have any effect on the splicing process of pre-mRNA. Using genomic DNA with direct sequencing of the polymerase chain reaction-amplified intron 24/exon 25 junction site, we found that 59 of the 60 patient samples were of the GTG sequence at nucleotides 6724-6726. The polymorphism is similar between populations in Taiwan and Western countries. The sequence of intron 24 around the splicing acceptor was always TCCAACTCTATTGCCCTCAG (-20 to -1), except for one hemophiliac patient who had a mutation in which the absolute consensus AG doublet of the intron 24 splicing acceptor changed to the AA dinucleotide. Owing to the mutation, exon 24 was erroneously spliced to exon 26, and exon 25 was skipped. This finding further testifies to the importance of the invariant AG dinucleotide in the example of the factor VIII gene.     

奎的平与氯氮平治疗精神分裂症对照研究

目的：探讨奎的平、氯氮平对精神分裂症的疗效及副作用。方法：80例患者随机分为两组，分别应用奎的平或氯氮平治疗6周，采用四级临床疗效评定标准、阳性症状与阴性症状量表(PANSS)、副反应量表(TESS)评定疗效及副反应。结果：奎的平组有效率87．5％，氯氮平组有效率82．5％。治疗第一周末，奎的平组PANSS量表总分较治疗前差异有显著性(P＜0．05)。奎的平组副反应低于氯氮平组。结论：奎的平与氯氮平疗效相似，但奎的平起效较快，副反应较轻。     

腹部平滑肌肉瘤CT诊断及鉴别诊断

目的探讨CT在腹部平滑肌肉瘤诊断中的作用。方法分析9例经手术病理证实的腹部平滑肌肉瘤的CT表现。结果9例平滑肌肉瘤发生于腹膜3例,胃3例,直肠、结肠及子宫各1例。肿瘤密度不均,中心有大片坏死,周围实质呈破絮状或环状,其中1例可见液平面。增强后为延迟边缘强化。结论CT检查对平滑肌肉瘤可做出较准确的诊断。     

实验性蛛网膜下腔出血后脑血管痉挛兔海马组织中Bcl-2和Bax mRNA的表达

目的：探讨蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)造成脑损伤的机制．方法：用反转录聚合酶链式反应(RT—PCB)技术检测兔SAH后CVS时海马组织Bcl—2和BaxmRNA的表达变化．结果：Bcl—2mBNA的表达水平在SAH组1d时即开始下降，3d时降至最低，持续至7d．SAH组海马组织中BaxmRNA的表达呈上升趋势，3d时达最高，7d时仍显著高于正常组．在假手术组海马组织内的Bcl—2和BaxmRNA的表达水平保持相对恒定．结论：Bcl—2和Bax可能参与了SAH后CVS所造成的海马神经元损伤过程。     

Retrospective case-analysis of the magnetic resonance imaging characteristics in the brain of patients with Wilson disease

BACKGROUND:Wilson disease (WD)damages liver,brain,kidney,cornea and nervous system severely.It is manifested in four ways:brain,liver,kidney and bone muscle.Whether or not magnetic resonance imagling (MRI)can clearly display the diseased region and range in brain of patient with WD,which provides imageological evidence for clinical practice,is unclear.OBJECTIVE:To observe the charactedstics of MRI of brain in patient with SD,and analyze the correlation of diseased region with clinical symptoms.DESIGN:Retrospective case-analysis.SETTING:Department of Radiology,Second Hospital Affiliated to the General Hospital of Chinese PLA.PARTICIPANTS:Thirty-one patients,including 18 males and 13 females,with WD admitted to the Department of Neurology,Second Hospital Affiliated to the General Hospital of Chinese PLA between January 1999and December 2005 were retrieved.The involved patients presented serum copper oxidase (sCP) activity decreasling and/or caruloplasmin Ievel decreasling and/or urinary copper content increasling;typical extrapyramidal symptoms and/or physical sign;abnormality showed by slit-lamp examination,Kayser-Fleischer rling positive.METHODS:①All the involved patients underwent MRI examination.A GE 1.5T imagling equipment was used.Spin-echo sequence was adopted to perform T2 and T1-weighed image at transverse axis level.Partial cases subjected to head scannling at coronal and/or sagittal level.Gd-DTPA With dosage of 0.1 mmol/kg was the strongest in 4 cases.②MRI characteristics of patients with dliferent clinical symptoms were observed.MAIN OUTCOME MEASURES:MRI detection results of patients with WD and MRI characteristics of patients with different clinical symptoms.RESULTS:Thirty-one patients with WD participated in the result analysis.①Imageological examination results:WD lnvolved many regions in the brain:dorsal caudate putamen(n=28),thalamencephalon(n=25),mesencaphalon(n=25),globus pallidus(n=23),pons(n=21),posterlor limb of intemal capsule(n=16),dentate nucleus(n=16),caudata nucleus(n=15)and cerebral cortex(n=11).MRI presented hypo-intensity signal on T2-weighted image and T1-weighted image or isointensity signal on T1-weighted image in 24 patients,characteristic hypo-intensity signal of globus pallidus in 4 patients,mixed signal of hyper-and hypo-intensity in 2 patients,hypo-intensity signal of globus pallidus,pars anterior pedunculi carebd and pontine tegmentum on T1-weighted image in 1 patient.Pathological changes distdbuted in symmetry and focus of infection mostly presented mottling,lamellar or strip.Different degrses of cerebral cortex atrophy,especially subtentodal cerebellar atrophy,ware found in 20 patients.Four patients subjected to enhancement scannling,but no clear imaqling was found.③MRI characteristics of patients with different symptoms:Abnormal signal of dorsal caudate putamen was found in 28 patients with dystonia,21 patients with dysarthda and 16 patients with bradykinesia;Abnormal signal of mesencaphalon was found in 22 patients with trepidation,among which,18 presented abnormal signal of pons;Abnormal signal of caudate nucleus and lenticular nucleus was found in 15 patients with dysphagia;Abnormal signal of dentate nucleus was found in 16 patients with carebellar ataxia;Different degrees of changes in cerebral atrophy were found in 14 patients with detedoratling memory and dementia.CONCLUSION:MRI can clearly display the characteristics of diseased regions in brain of patient with WD.Diseased regions reflected by MRI have obvious differences in patients with different clinical neurosigns.