癌光啉对动物移植瘤的光化学诊治作用

本文报道了肿瘤光化学诊治新药癌光啉(PsD-007)对三种动物移植瘤(S_(180)肉瘤、Lewis肺癌、U_(14)宫颈癌)的实验性光化学诊治效果,初步探讨了辐照光能量和药物剂量与肿瘤实验性诊治效果的关系。作者采用“透镜光阑”法改善辐射光斑内功率密度分布的不均匀性。提供了一种能比较客观地评价药物对动物移植瘤的光动力学治疗效价的模型体系。     

VALUE AND LIMITATIONS OF SINUS NODE FUNCTION TE.ST BY ATRIAL PACING IN SICK SINUS SYNDROME DIAGNOSIS

In order to evaluate the significance of the sinus node function test by transvenous atrial pacing in the diagno,sis of sick sinus syndrome (SSS), sinus node recovery time (SRT), co]rrected sinus node recovery time (CSRT) and total atrio- sinus and sino-atrial conduction time (SACT) are observed in 69 patients including non-SSS group 39 cases and SSS group 30 cases. According to our data, we suggest criteria for sinus node dysfunction as follows. SRT>1,400 ms and/or A-V junctional esca.pe before sinus recovery and;or secondary pause, CSRT>560 ms. total SACT>300 ms. With l item abnormal, the false positive rat.e in the non-SSS group is 7.7c7。 and the false negative rate in the S.SS group 3.30i" with 2 items abnormal, the false positive rate in the non-SSS group Oi and the false negative rate in the SSS grOiup 6.7'70. Atropine test results are compared with that of atrial pacing, 95.8To and 84.2% are compatible with each other in these 2 groups. In order to cut the use of invasive technic to the minimum, we propose performing the atropine test first. Only in those with unexplainable test results should the trial pacing test. be resorted to..     

How is your life? understanding the relative importance of life domains amongst older adults,and their associations with self-perceived COVID-19 impacts

Quality of Life Research - This study aims to provide new knowledge on the relative importance of key life domains amongst older adults, and how the Coronavirus pandemic has influenced their life...     

Discrete choice experiment with duration versus time trade-off: a comparison of test–retest reliability of health utility elicitation approaches in SF-6Dv2 valuation

Quality of Life Research - To evaluate and compare the test–retest reliability of discrete choice experiments with duration (DCETTO) and time trade-off (TTO) in the Chinese SF-6Dv2 valuation...     

Pharmaceutical Sales Representatives in the United States and China: The Need for Professional Public Space

Health Care Analysis - Pharmaceutical sales representatives (PSRs) are one of the most frequently used drug information sources for physicians in both the United States and China. During...     

痛泻要方水煎液化学成分的UPLC-LTQ-Orbitrap-MS分析

目的：采用超高效液相色谱-线性离子阱-静电场轨道阱高分辨质谱法(UPLC-LTQ-Orbitrap-MS)快速鉴定痛泻要方水煎液中的化学成分。方法：色谱条件为ACQUITY UPLC BEH C₁₈色谱柱(2.1 mm×100 mm,1.7μm),流动相0.1%甲酸水溶液(A)-乙腈(B)梯度洗脱(0～4 min,5%～15%B;4～10 min,15%～25%B;10～15 min,25%～60%B;15～20 min,60%～90%B;20～25 min,90%～100%B;25～27 min,100%B;27～30 min,100%～5%B;30～32 min,5%B),流速0.3 m L·min^-1,进样量3μL,柱温35℃。质谱条件为电喷雾离子源(ESI),扫描范围m/z 100～1 250,正、负离子模式下分别采集MS、MS/MS数据。结合对照品、数据库和文献信息,运用Trace Finder 4.1和Xcalibur 2.1软件对痛泻要方水煎液进行化学成分鉴定。结果：在正、负离子模式下,从痛泻要方水煎液中共鉴定出90个化合物,主...     

基于转录组数据的半夏AP2/ERF基因家族鉴定及逆境响应分析

目的：鉴定并研究半夏AP2/ERF转录因子的功能,为推进半夏品种的遗传改良提供理论依据。方法：该文基于半夏三代转录组数据鉴定了半夏中AP2/ERF家族成员,并对其进行系统的生物信息学分析,同时利用实时荧光定量聚合酶链式反应（Real-time PCR）检测半夏AP2/ERF在不同组织及不同胁迫条件下的表达情况。结果：通过转录组数据共筛选出8个全长AP2/ERF转录因子家族成员,归为AP2、ERF和DREB 3个亚家族;半夏AP2/ERF的氨基酸数目为251～512个,等电点为5.29～11.72,不稳定指数为45.90～82.41,且主要定位于细胞核中;半夏AP2/ERF基因的结构域和Motifs相对保守。组织特异性表达模式分析显示,半夏AP2/ERF基因在不同组织部位中具有不同的表达模式,且主要在叶中表达。逆境胁迫应答分析表明,PtERF1主要响应NaCl的胁迫诱导;PtERF2和PtERF4在低温和聚乙二醇（PEG）胁迫下表达量均有较大幅度的上调;PtERF3同时响应低温和NaCl两种逆境的诱导;PtERF5同时响应高温、低温、NaCl、PEG胁迫诱导;PtERF7在高温胁迫下表达...     

Associations between Circulating Markers of Cholesterol Homeostasis and Macrovascular Events among Patients Undergoing Hemodialysis

Current strategies targeting serum cholesterol bring limited benefits to mortality and macrovascular events prevention among hemodialysis patients. Direct measurements and analysis on circulating markers of cholesterol homeostasis could be promising solutions to this bottleneck. We prospectively enrolled 90 maintenance hemodialysis patients and 9 healthy controls in 2019 for 1 year. We measured circulating desmosterol and lathosterol as markers for cholesterol synthesis and campesterol and sitosterol for cholesterol absorption. At baseline, hemodialysis patients showed higher levels of campesterol (p = 0.023) compared to healthy controls. During follow-up, we identified 14 (15.4%) patients who experienced macrovascular events. Comparisons of cholesterol homeostasis markers between cohorts with and without macrovascular events showed no significant differences in markers of cholesterol synthesis or absorption. Using logistic regression analysis, the odds ratio was not statistically significant for the prediction of macrovascular events after full-adjusting for age, sex, diabetes, serum albumin, cholesterol, and triglyceride. We concluded that hemodialysis patients demonstrated higher level of cholesterols absorption, indicated by circulating campesterol compared to healthy subjects. Markers for cholesterol homeostasis were not significantly associated with macrovascular events during a 1-year follow-up. Our results shed light on the novel therapeutic target of modulating cholesterol absorption in HD patients.