氯胺酮和氯诺昔康对慢性关节炎疼痛治疗的实验研究

目的研究氯胺酮、氯诺昔康及两药联合关节内注射对慢性关节炎疼痛的疗效．方法大鼠右踝关节注射完全弗氏佐剂建立单发性关节炎模型，4周后，关节内注射氯胺酮、氯诺昔康及两药联用，每周3次，共用药2周．踝关节X线照片、关节病理切片、关节直径值、屈关节疼痛试验评分和血浆谷氨酸含量为指标．结果踝关节X线照片和病理切片结果提示慢性炎症存在．用药前实验组右踝关节直径值、屈关节疼痛试验评分、血浆谷氨酸含量显著高于对照组（P〈0．05）；用药2周3个用药组疼痛评分、血浆谷氨酸含量显著低于非用药组，其中氯诺昔康组和联合组右踝关节直径显著低于非用药组（P〈0．05），且2组关节病理切片均见到有新生关节软骨的形成．结论关节内注射氯胺酮和氯诺昔康均可缓解慢性关节炎疼痛，氯诺昔康较氯胺酮抗炎作用明显，还可促进受损关节的修复，联合注射氯胺酮和氯诺昔康，可大大减少两药的用量．     

性别特异性高胆固醇血症与Syndecan-3(SDC3)基因C1330T多态性的关联研究

目的研究syndecan-3(SDC3)基因的单核苷酸多态性(single-nucleotide polymorphism,SNP)C1330T在中国汉族农村人群中与高胆固醇血症,血脂水平的相关性。方法高胆固醇血症(HTC)定义为血清中总胆固醇水平≥200 mg/dL。本研究中男性336人(其中HTC161人,胆固醇正常者(NTC)175人),女性1491人(其中HTC935人,NTC556人)。研究对象中应用多聚酶链反应(PCR),限制性内切酶酶切进行基因分型,测定血清高密度脂蛋白(HDL-C),低密度脂蛋白(LDL-C),总胆固醇(TC),甘油三脂(TG),葡萄糖(GLU)。结果在男性研究对象中SDC3基因C1330T基因型及等位基因频率在HTC组和NTC组的分布有显著性差异(P<0.05),1330C与高胆固醇血症相关(OR=1.42,95%CI=1.040-1.926,P<0.03),在校正年龄,BMI和其他危险因素(吸烟饮酒等)后仍保持显著相关(OR=1.51,95%CI=1.06-2.14,P<0.03),而在女性研究对象中未发现明显相关(OR=1.06,95%CI=0.92-1.24,P=0.41),1330C与性别的交互作用呈临界统计学显著性(borderline statistically significant)(P=0.0742)。进一步应用显性模型分析C1330T与男性血脂水平(HDL-C,LDL-C)相关。结论SDC3基因C1330T多态性与男性高胆固醇血症及血脂水平有关。     

中药酚性化合物的抗肿瘤机制及制备方法

酚性化合物是许多中药的有效成分,具有抗肿瘤作用。其作用机理为抗氧化作用,抗细胞增殖,诱导细胞调亡,干预细胞转导和增强抑癌基因表达,干预细胞周期等。利用现代分离技术可将其提取并纯化。就酚性化合物抗肿瘤机理和提取纯化方法进行综述。     

2006年珠海市围产儿出生缺陷监测及干预研究

目的了解珠海市围产儿出生缺陷现状,探讨出生缺陷发生的相关因素,降低出生缺陷和残疾发生率,提高出生人口素质。方法根据珠海市出生缺陷监测方案,对全市35家医疗机构围产儿进行监测。结果出生缺陷发生率男性高于女性(X2=15.64,P<0.001),城市高于农村(X2=6.39,P=0.01),年龄<20岁的孕妇出生缺陷发生率明显高于其它人群,妇女最佳受孕年龄是20∽25岁。珠海市发生率最高的前5种疾病排序依次是:骨关节畸形、先心病、泌尿生殖系统畸形、唇腭裂、外耳畸形。结论珠海市围产儿出生缺陷发生率呈上升趋势。孕前期及孕妇早期口服小剂量叶酸预防神经管畸形,提高产前筛查和产前诊断技术,加强孕期保健,提高环保意识,这些是降低出生缺陷发生的有力措施。     

Primary Culture of Alveolar Epithelial Type Ⅱ Cells and Its Bionomic Study

To establish a better method of primary culture for alveolar epithelial type Ⅱ cells (AEC Ⅱ) and to study its bionomics, alveolar epithelial type Ⅱ cells were isolated by digestion with tryp- sin and collagenase, which were then purified by plated into culture flask coated with rat immu- noglobulin G. The purified AEC Ⅱ were identified by alkaline phosphatase staining, electron mi- croscopy, immunocytochemical staining of pulmonary surfactant protein A (SPA). The SPA expres- sion and transfection characteristics were compared with those of A549 cell line. The results showed that AEC Ⅱ could be isolated by digestion with trysin and collagenase and purified by adhesive pu- rification by using IgG, with a yield of about 2–3×107, and a purity of about 75%–84 %. Cells could be quickly identified with AKP staining. AECⅡ were different from A549 cell line in terms of SPA expression and transfection characteristics. It is concluded that adhesive purification with IgG can improve the purity of AECⅡ, and AKP staining is simple in cell identification. AECⅡ can not be completely replaced by A549 cells in some studies because the differences between them, such as SPA expression.     

Determination of Carboplatin Dose by Area Under the Curve in Combination Chemotherapy for Senile Non-small Cell Lung Cancer

To preliminarily determine the appropriate dosage of carboplatin (CBP) at AUC of 5 mg·Ml-1·min-1 in the combination chemotherapy for Chinese senile patients with non-small cell lung cancer (NSCLC). Thirty-five Chinese senile patients with NSCLC in advanced stage (Ⅲ/Ⅳ) were given 96 cycles of combination chemotherapy. Chemotherapy schedules included Taxol CBP, Gemzar CBP and NVB CBP. The dose of CBP was at 5 mg·mL-1·min-1 of area under the concen- tration-time curve (AUC). Side effects and quality of life were observed before and after the chemo- therapy. Myelosuppression was severe and commonly observed. Grade 3/4 of granulocytopenia was found in 47.9% (46/96) of the patients and grade 3/4 of thrombocytopenia was noted in 28.1% (27/96) of the subjects. However, other side effects were slight. The mean score of quality of life (QOL), ac- cording to the criteria of QOL for Chinese cancer patients had reduced 6.8. At 5 mg·mL-1·min-1 by AUC, the hematological toxicity of CBP was severe and it had some negative effects on the QOL. The administration of CBP at 5 mg·mL-1·min-1 by AUC may be too high for Chinese senile patients with non-small cell lung cancer.     

MDR1 and MDR3 Genes and Drug Resistance to Cisplatin of Ovarian Cancer Cells

To investigate the relationship between MDR1 and MDR3 gene and drug resistance to cisplatin of ovarian cancer cells. Two siRNAs (MDR1, MDR3) which specifically targeted MDR1 and MDR3 genes were transfered into A2780/DDP cells. Then double staining with Annexin- V-FITC/PI was used to detect cell apoptosis by the flow cytometry (FCM). A2780/DDP cell viability was determined by MTT. MDR1 and MDR3 mRNA were assessed by RT-PCR. Caspase-3 protein was detected by Western blotting. Transfection of MDR1 and MDR3 siRNA into A2780/DDP cells failed to reverse the drug-resistance of A2780/DDP cells to cisplatin (P>0.05). No significant differ- ence in the apoptosis efficiency was observed between the MDR1 and MDR3 siRNA, pSuppressor- Neo vector transfection cells and untreated cells (P>0.05). In the presence of cisplatin of different concentrations, the viability of A2780/DDP cells was not significantly decreased after the transfection. No changes in MDR1 and MDR3 mRNA were found in MDR1 and MDR3 siRNA-transfected A2780/DDP cells. As compared with pSuppressorNeo and untreated groups, no significant difference existed in the expression of MDR1 and MDR3 mRNA (P>0.05). The expression of caspase-3 protein in MDR1 and MDR3 siRNA transfected A2780/DDP cells was not significantly increased. It is con- cluded that multidrug resistance induced by cisplatin in ovarian carcinoma cell lines is not due to overexpression of MDR1 and MDR3 gene. The drug resistance of ovarian carcinoma cells to cisplatin is not mediated by P-glycoprotein.     

鼻出血患者100例治疗结果分析

目的总结鼻出血的治疗经验,分析治疗中可能存在的问题,探讨各种治疗措施在处理鼻出血中的意义。方法对2006年5月～12月来住院治疗的鼻出血患者100例进行回顾性分析与研究。结果100例均治愈,治疗手段包括前后鼻孔填塞、内镜下电凝、微波、药物烧灼手术等。结论治疗鼻出血的关键是找准出血部位及明确出血原因,再根据患者的实际情况采取有效止血措施。     

上海地区直燃型溴化锂机组与水冷螺杆冷水机组运行费用比较

根据上海地区目前的能源价格,比较直燃型溴化锂机组与水冷螺杆冷水机组的运行费用,为设计过程中冷水机组形式的选择提供参考数据。     

学术期刊与法律法规

学术期刊在办刊中也应注重法律法规,重视党和国家政策的宣传,期刊法人代表,负责人要依法执业,增强法律意识,就目前历史时期期刊经管、管理中存在的问题和相关思考提出了看法。