实时荧光聚合酶链反应检测HBV基因型B～D方法的建立

目的建立实时荧光聚合酶链反应检测乙型肝炎病毒(HBV)基因型B～D方法并验证其准确性。方法比较GenBank中已发表的明确分型的143株HBV全序列，设计特异的组合引物探针，应用实时荧光聚合酶链反应HBV基因分型法对兰州地区128例慢性乙型肝炎患血清标本进行基因型B、C、D检测，并随机对检测的基因型各3株标本进行S基因测序验证。结果128例标本中B型检出率为20.3％(26／128)，C型71．9％(92／128)，D型7．8％(10／128)：18株HBV克隆标本S基因测序结果与本分型法完全一致。结论实时荧光聚合酶链反应HBV基因分型法能够简便、灵敏、快速、准确地鉴定HBV基因型，适宜用于HBV基因型的大规模临床和流行病学调查。     

听神经瘤手术无术中电生理监测40例的面/听神经功能保护

目的 探讨听神经瘤的显微外科手术技巧及术中无电生理监测辅助时的面/听神经功能保护.方法 回顾性分析经影像学检查诊断,应用显微神经外科技术,经枕下-乙状窦后入路行肿瘤切除,术中无电生理监测的40例听神经瘤病例,讨论患者面/听神经功能保护.结果 镜下肿瘤全切38例(95.0%),次全切和大部切除2例(5.0%);术中面神经...     

Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice

Objective： To investigate the effect of Qingre Quyu Granule （清热祛瘀颗粒, QRQYG） on stabilizing vulnerable plaques in apolipoprotein E （ApoE） defficient mice. Methods： Seventy-two male ApoE defficient mice were given a high-fat diet from 6 weeks of age. At the 16th week, all the mice were randomized into 3 groups： the QRQYG group, the simvastatin group, and the control group. Sixteen weeks after administration of 0.9 g/kg QRQYG, 3 mg/kg simvastatin or 10 mg/kg sodium chloride per day to the respective groups, the animals were euthanized. The pathological morphologic changes in the vulnerable plaques were evaluated, the matrix metalloprotease-9 （MMP-9） expression was measured by immunohistofluorescence, the soluble intercellular adhesion molecule 1 （ICAM-1） was determined by ELISA, the nuclear factor kappaB （NF-κB） subunit p65 was measured by quantitative RT-PCR, and, finally, thrombospondin-1 （TSP-1） was determined by the immunohistochemical method. Results： The plaque cross-sectional area in the brachiocephalic artery （23.7%, P0.01）, the lipid core of the plaque （43.1%±3.1%）, and the number of buried ？brotic caps of the plaque were significantly decreased in the QRQYG group compared to the control group （both P0.01）; furthermore, the thickness of the ？brotic cap of the plaque increased and the intra-plaque hemorrhage of the plaque decreased. The serum soluble ICAM-1 （27.1±5.1 μg/mL）, the protein expression of MMP-9 and TSP-1 and the p65 mRNA expression increased in the QRQYG group in comparison with the control group （P0.05 or P0.01）. Conclusion： QRQYG could stabilize the vulnerable plaque through inhibition of the inflammatory response.     

益肾颗粒剂对狼疮性肾炎患者血清IL-6的影响

目的观察益肾颗粒剂对狼疮性肾炎患者的血清IL-6及临床生化指标的影响。方法将40例狼疮性肾炎患者随机分为治疗组(益肾颗粒剂组)及对照组(强的松组),对比服药4周前后两组患者的血清IL-6及其24h尿蛋白定量、血清尿素氮、肌酐。结果两组患者治疗前后血清IL-6及其24h尿蛋白定量、血清尿素氮、肌酐等指标均有明显改善(P<0.05),且两组患者之间治疗后血清IL-6及24h尿蛋白定量均存在明显差异(P<0.05)。结论益肾颗粒剂对于狼疮性肾炎有改善治疗作用。     

Construction of AP003469.4-miRNAs-mRNAs ceRNA network to reveal potential biomarkers for hepatocellular carcinoma

Studies have reported that the competing endogenous RNA (ceRNA) networks are related to disease progression and prognosis in patients with hepatocellular carcinoma (HCC). The roles and mechanisms of long-chain non-coding RNA AP003469.4 in HCC have remained unclear. Here, we explored the roles of AP003469.4 in HCC progression using bioinformatics, CCK-8, Transwell assay, etc. AP003469.4 targets miRNAs and these target genes were predicted by the LncBase Predicted v.2, miRDB, miRTarBase, and TargetScan databases. Then, AP003469.4-associated ceRNA network was constructed. Biological functions and mechanisms of differentially expressed genes in the ceRNA network were explored using GO and KEGG. Survival analysis and Cox regression analysis were used to screen prognostic genes and construct a prognostic risk model. The results revealed that AP003469.4, with the area under the curve of 0.9048, was highly expressed in HCC tissues. Increased expression of AP003469.4 was an independent risk factor for the dismal prognosis of HCC patients and was associated with the short overall and disease-free survival. Downregulation of AP003469.4 expression inhibited cell proliferation, cycle transition, invasion, and migration, and promoted cell apoptosis. There were 489 differentially expressed target genes in the ceRNA network, which were involved in several pathways, such as the MAPK signaling pathway, cell cycle, and p53 signaling pathway. The risk model was based on the DTYMK, ZFC3H1, CBX2, PKM, TTC26, ATG10, TAGLN2, CD3EAP, SHISA9, SLC1A5, KPNA2, SCML2, E2F7, and SMARCD1, which were the independent risk factors for poor prognosis of HCC patients. In general, interference with AP003469.4 expression might delay the progression of HCC. AP003469.4 related network could help to identify the hub target molecules in HCC progression, which might be candidate biomarkers for evaluating the prognosis of HCC patients.     

肺炎性假瘤及球形肺结核细胞增殖研究

目的了解肺炎性假瘤及球形肺结核是否存在细胞增殖异常。方法用流式细胞仪对9例患者(其中5例肺炎性假瘤、4例球形肺结核病)痰液细胞DNA含量进行分析,并与痰细胞学检查、手术及经皮肺活检组织P53、PCNA、Ki67免疫组化分析结果作比较。结果以最后病理诊断为标准,肺炎性假瘤及球形肺结核患者异倍体检出率分别为40%(2/5)与50%(2/4),其相应的病变组织切片PCNA、Ki67免疫组化染色也阳性,但P53免疫组化染色阴性。结论部分炎性假瘤及结核球存在细胞增殖异常。痰细胞DNA含量分析是发现增殖异常的有效辅助方法,可能有利于肺癌的早期诊断。     

肺癌三维适形放疗与常规放疗剂量学优势的比较

目的:比较肺癌三维适形放射治疗与常规放射治疗时的肿瘤靶区、正常组织和器官的剂量分布优势。方法:选择三维适形放射治疗进行根治性治疗的10例肺癌,先在适形放射治疗体位下在模拟机下常规定位,标出其射野范围后进行CT模拟扫描。非小细胞肺癌在DT40Gy时行第2次CT扫描调整CTV,常规照射设野避开脊髓加量。采用三维适形治疗计划系统评价常规照射和三维适形照射对肿瘤靶区、正常组织和器官的剂量分布。结果:三维适形放射治疗比常规放射治疗,从剂量分布对肿瘤靶区有更好的适形度,95%CTV达到的平均剂量高出11·5Gy(5~20Gy),平均高出医生给予剂量CTV体积的38%(10%~59%)。在正常组织中,除健侧肺的V20和V30比常规放疗稍高外(1·1%和1·7%),脊髓的最大剂量平均低4Gy(-4~12Gy),心脏V35低24·3%(-4%~75%),双肺V20和V30分别低7%(-5%~20%)与7·4%(-6%~20%),患侧肺的V20和V30分别低13%(-7%~45%)和15%(-8%~40%)。结论:三维适形放疗治疗肺癌比常规放射治疗有一定的优势,对肿瘤靶区有更好的适形度和剂量均匀性,并且能更好的减少正常组织脊髓、心脏和肺的照射体积和剂量,特别是高剂量的照射体积。     

丹参素对高糖刺激腹膜间皮细胞分泌纤维连接蛋白和I型胶原的影响

目的:探讨丹参素对高糖刺激培养的腹膜间皮细胞(HPMCs) 分泌纤维连接蛋白(FN)和I型胶原(Col-I)的影响.方法:以高糖刺激培养HPMCs,分别加用浓度为10,5,2.5,1.25,0.625 mg/L的丹参素进行干预,并选取浓度为10 mg/L的丹参素干预0,12,24,48,72 h;应用RT-PCR,EL...     

Perioperative analysis of laparoscopic liver resection with different methods of hepatic inflow occlusion

目的 探讨腹腔镜肝切除术中三种肝门阻断方式的手术效果.方法 回顾性分析应用三种不同的肝门阻断方式的腹腔镜肝切除术病人临床资料,其中全肝血流阻断法(Gro-IPO组)10例,即Pringle法,半肝血流阻断(Gro-HVO组)10例,选择性的肝门阻断(Gro-SVO组)11例.对病人术中出血量、手术时间、阻断时间、并发症发生情况进行统计学分析,比较其优缺点.结果 31例手术均获得成功,无死亡及中转开腹病例.三组在术中出血、手术时间上未见明显差异,全肝血流阻断时间Gro-IPO组短于其他两组,且差异有统计学意义(P＜0.05).结论 选择性的肝门阻断在腹腔镜肝切除中是安全可行的,HVO法适用于左半肝或左外叶的切除,而SVO法适用于肝右叶的切除.     

自体髂骨再造桡骨头维持肘关节稳定的实验研究

目的利用兔动物模型进行实验研究,评价桡骨小头切除后用自体髂骨与尺骨冠突桡侧面融合再造桡骨头的骨愈合效果。方法选用健康成年新西兰白兔24只,随机分为实验组和对照组,每组各12只。实验组切除单侧桡骨头颈,取自体髂骨块行桡骨头再造。对照组在麻醉后行传统单侧桡骨小头切除术。2组在术后第4、8、12周分别处死4只白兔,通过大体标本测量肘关节外翻角的变化,进行影像学和骨组织形态计量分析观察骨愈合的效果。结果 2组肘关节外翻角随观察时间延长而逐渐增加,对照组增加幅度相对较大,而实验组增加幅度相对更小。大体标本肘关节外翻角测量(肘伸直位):术后12周时,对照组桡骨小头切除侧为(19.6±2.8)°,实验组桡骨头切除重建侧为(5.6±1.8)°,组间差异有统计学意义(t=4.004,P=0.028)。对照组术后均未见明显骨痂形成,实验组术后8周骨痂面积X线评分显著高于术后4周(t=5.175,P=0.014),术后12周显著高于术后8周(t=8.891,P=0.003)。实验组术后4周骨融合面部分软骨痂形成,术后8周再造骨融合面有较多骨痂形成,骨板排列无规律,术后12周再造骨融合面有大量新生骨痂形成;对照组桡骨小头切除后缺损处仅为纤维组织填充。结论改良桡骨头切除自体髂骨再造重建手术治疗Mason Ⅲ型桡骨小头骨折安全可行,自体髂骨块再造桡骨头能与尺骨冠突桡侧面牢固愈合,术后稳定性及融合效果良好。