Enzyme spectrum of the pancreas in chronic stress

The enzyme spectrum of pancreatic homogenate was studied in acute experiments on male albino rats during adaptation for 30 days to muscular exertion (forced swimming in water at a temperature of 32±1°C), heat (hyperthermia to 40–41°C), and cold (cooling to 3–4°C) for 3 h. The initial periods of adaptation to these factors (second-twelfth day) were shown to be characterized by a considerable decrease in activity of all the enzymes studied, but later, with adaptation of the animals to these factors, enzyme activity was restored to its original level (18th–24th day), and remained more or less constant until the end of the experiment (30th day). It is suggested that changes in the enzyme spectrum of the pancreas are brought about through the participation of the hypothalamo-hypophyseo-adrenal system in accordance with the principle of the general adaptation syndrome.Laboratory of the Physiology of Digestion and Laboratory of General Physiology, Institute of Physiology, Academy of Sciences of the Uzbek SSR, Tashkent. (Presented by Academician of the Academy of Sciences of the USSR O. G. Gazenko.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 87, No. 5, pp. 412–414, May, 1979.     

Aktivitätsverminderung der post-Heparin-Lipoproteinlipase bei azidotischem Blut-pH

Summary Diseases associated with acidotic blood-pH, such as chronic renal disease, diabetes mellitus or chronic alcoholism, show a marked impairment of lipoprotein lipase. Therefore we influenced blood-pH in 3 healthy subjects by infusions to get alkalotic, neutral and acidotic blood-pH on three days in series. On each day blood-pH from capillary blood and post-heparin lipoprotein lipase from fasting plasma was determined. In comparison to neutral blood-pH in vivo, alkalosis did not influence lipoprotein lipase. In contrast, during artificial acidosis, lipoprotein lipase was impaired significantly (p<0.01). Therefore, it seems, that acidosis inhibits lipoprotein lipase in vivo.

     

Alternative methods to analyse the impact of HIV mutations on virological response to antiviral therapy

Background  

Principal component analysis (PCA) and partial least square (PLS) regression may be useful to summarize the HIV genotypic information. Without pre-selection each mutation presented in at least one patient is considered with a different weight. We compared these two strategies with the construction of a usual genotypic score.     

Bases anatomiques des vagotomies

Résumé La réalisation des différentes vagotomies, tronculaires, sélectives, supra-sélectives, nécessite la connaissance de l'innervation parasympathique de l'estomac.La disposition modale, les variations anatomiques et les données les plus récentes des constations per-opératoires sont analysées dans cet article.     

Expression of “early” and “late” viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Summary A somatic cell hybrid (Cl. 6d) was originated from the fusion of mouse (3T3-4E) and spontaneous yielder SV 40-transformed Chinese hamster (CHK/SVLP AG) cells. During the early stages of its history, the Cl. 6d hybrid underwent a rapid chromosome loss, preferentially loosing hamster chromosomes. This was not a constant tendency of the hybrid cells.As the parental CHK/SVLP AG cells, the hybrid cells were always found 100 per cent SV 40 T-antigen positive. While in CHK/SVLP AG cells infectious SV 40 DNA, V-antigen and virus were regularly detected, in the hybrid cells only infectious DNA was occasionally detected. This was not due either to the loss of an essential Chinese hamster gene(s) or to the presence of an inhibiting mouse cell component(s); it was apparently the consequence of inability of the cells to properly activate the resident SV 40 genome(s).After superinfection with SV 40 DNA, the hybrid cells—though capable of synthesizing SV 40 V-antigen—were unable to ensure virus assembly. Experimental evidence was obtained suggesting that SV 40 maturation is dependent on a cellular function(s).With 1 Figure