Reduced Expression of SARM in Mouse Spleen during Polymicrobial Sepsis

Objective Immune dysfunction, including prominent apoptosis of immune cells and decreased functioning of the remaining immune cells, plays a central role in the pathogenesis of sepsis. Sterile α and HEAT/armadillo motif-containing protein (SARM) is implicated in the regulation of immune cell apoptosis. This study aimed to elucidate SARM contributes to sepsis-induced immune cell death and immunosuppression. Methods A mouse model of polymicrobial sepsis was generated by cecum ligation and puncture (CLP). SARM gene and protein expression, caspase 3 cleavage and intracellular ATP production were measured in the mouse spleens. Results CLP-induced polymicrobial sepsis specifically attenuated both the gene and protein expression of SARM in the spleens. Moreover, the attenuation of SARM expression synchronized with splenocyte apoptosis, as evidenced by increased caspase 3 cleavage and ATP depletion. Conclusions These findings suggest that SARM is a potential regulator of sepsis-induced splenocyte apoptosis.     

Impact of JAK2V617F Mutation Burden on Disease Phenotype in Chinese Patients with JAK2V617F-positive Polycythemia Vera (PV) and Essential thrombocythemia (ET)

Most patients with polycythemia vera (PV) and half of essential thrombocythemia (ET) possess an activating JAK2V617F mutation. The objective of this study was to better define the effect of JAK2V617F mutant allele burden on clinical phenotypes in Chinese patients, especially thrombosis. By real-time polymerase chain reaction (RT-PCR), the JAK2V617F mutation burden was detected in 170 JAK2V617F-positive patients, including 54 PV and 116 ET. The results showed that JAK2V617F allele burden was higher in PV than in ET (P< 0.001). Higher percentage of patients had JAK2V617F allele burden over 20% in PV than in ET (68.5% VS 26.7%) (P< 0.001). In PV patients, higher JAK2V617F allele burden was observed in female (P< 0.05) and leukocytosis patients (WBC above 10×10⁹/L) (P< 0.001). Meanwhile, ET patients showed increased JAK2V617F allele burden in the group with higher hemoglobin (HGB above 150g/L) (P< 0.05), leukocytosis (WBC above 10×10⁹/L) (P< 0.001), splenomegaly (P< 0.05) and thrombosis (P< 0.05). In conclusion, the JAK2V617F mutation allele burden is higher in Chinese patients with PV than ET. In PV patients, JAK2V617F mutation burden had influence on WBC counts. And the clinical characteristics of ET patients, such as WBC counts, hemoglobin level, splenomegaly and thrombosis, were influenced by JAK2V617F mutation burden. Male, high hemoglobin (HGB above 150g/L), and increased JAK2V617F mutation burden (JAK2V617F allele burden ≥16.5%) were risks of thrombosis (P< 0.05) for ET patients by Logistic Regression.     

非小细胞肺癌影像学特征与基因表达间相关性的探索性研究

影像基因组学通过挖掘肿瘤的影像和基因组学间的关联性,将两者的优势互补,以此指导不同患者个体化治疗方案的制定、预后评估、疗效检测等。针对非小细胞肺癌(NSCLC),建立其CT影像定量特征与基因表达之间的映射。首先,将对应的CT影像中肿瘤区域进行分割和特征提取,选用66种三维定量特征作为肿瘤区域影像特征集;然后,利用基因组学数据分析流程,在原始基因数据经过预处理、聚类后,获取其第一主成分作为具有相似表达谱基因聚类结果的代表;最后,运用基因芯片显著性分析算法探寻两者之间的相关性,并进行基因集的富集分析和预测模型的建立。对癌症图像归档数据库(TCIA)中的26例NSCLC影像数据和基因表达综合数据库(GEO)中相对应的基因数据进行分析,共得到126组成对的显著关联(q<0.05)。将所得结果中的29组元基因建立预测模型,并通过TCIA中更新的211组数据,对其中10组预测准确率大于70%且预测的元基因有生物学意义的预测模型进行验证,最终预测准确率为35.48%～80.85%,10个预测模型中有6个的准确率在70%以上。实验结果表明,特定的影像特征或其组合可以作为基因表达的影像标记物。     

A comparative study of proliferation and osteogenic differentiation of adipose-derived stem cells on akermanite and beta-TCP ceramics

This study investigated the in vitro effects of akermanite, a new kind of Ca-, Mg-, Si-containing bioceramic, on the attachment, proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs). Parallel comparison of the cellular behaviors of hASCs on the akermanite was made with those on beta-tricalcium phosphate (beta-TCP). Scanning electron microscope (SEM) observation and fluorescent DiO labeling were carried out to reveal the attachment and growth of hASCs on the two ceramic surfaces, while the quantitative assay of cell proliferation with time was detected by DNA assay. Osteogenic differentiation of hASCs cultured on the akermanite and beta-TCP was assayed by ALP expression and osteocalcin (OCN) deposition, which was further confirmed by Real-time PCR analysis for markers of osteogenic differentiation. It was shown that hASCs attached and spread well on the akermanite as those on beta-TCP, and similar proliferation behaviors of hASCs were observed on the two ceramics. Both of them exhibited good compatibility to hASCs with only minor cytotoxicity as compared with the tissue culture plates. Interestingly, the osteogenic differentiation of hASCs could be enhanced on the akermanite compared with that on the beta-TCP when the culture time was extended to approximately 10 days. Thus, it can be ascertained that akermanite ceramics may serve as a potential scaffold for bone tissue engineering.     

微创克氏针置入并外固定架修复桡骨远端不稳定骨骺骨折

背景：单纯使用克氏针或外固定架治疗桡骨远端骨骺骨折的临床实践中,仍存在桡骨短缩、骨折移位等固定后并发症,尤其在不稳定骨骺骨折情况下。克氏针主要治疗桡骨远端骨折,对其骨骺骨折使用相对较少。 目的：观察外固定支架结合经皮克氏针复位固定微创治疗桡骨远端不稳定性骨骺骨折的临床效果。 方法：对90例桡骨远端骨骺骨折患儿行固定治疗,在不切开情况下微创闭合复位骨折骨骺,随机分成2组,对照组采用单纯跨腕关节外固定支架固定方案;观察组采用跨腕关节外固定支架结合经皮克氏针闭合帮助复位固定骨骺骨折方案。固定后行腕关节功能锻炼,分别于固定后9周及24个月随访观察。对比两组患者固定后中远期的临床疗效、腕关节功能恢复及X射线检查情况。 结果与结论：固定后24个月随访按Cooney标准评定腕关节功能,对照组优良率77%,X射线评定优良率63%;观察组腕关节功能优良率93%,X射线评定优良率为90%,两组差异具有显著性意义(P < 0.01)。两组骨骺骨折患者均获临床骨愈合,腕关节均功能恢复。提示外固定支架结合克氏针闭合复位微创治疗桡骨远端不稳定骨骺骨折,可应用克氏针辅助复位掌倾角及尺偏角,治疗简单,固定可靠,腕关节功能恢复良好,骨骺畸形愈合并发症少,固定后基本生活学习功能恢复正常,疗效稳定满意,其临床疗效明显优于单纯跨外固定支架固定。     

动脉粥样硬化斑块形成时血管平滑肌细胞增生相关基因的cDNA全长克隆及其功能的初步探讨

目的　克隆动脉粥样硬化斑块形成时血管平滑肌细胞（ＳＭＣ） 增生相关基因的ｃＤＮＡ 全长,并对其功能进行初步探讨。方法　选用氧化低密度脂蛋白（ｏｘＬＤＬ） 作为刺激物作用于培养的人主动脉ＳＭＣ,利用减除杂交技术构建减除文库,克隆相关基因片段,在全长文库构建的基础上筛选相关基因的ｃＤＮＡ全长。并进行原核系统内蛋白表达及量效关系检测。结果　发现了４ 个新基因片段,克隆了一个新基因ｃＤＮＡ 全长,该基因在原核系统内有大约相对分子质量为４４ ０００ 的蛋白表达,其高表达可能与ＳＭＣ增生有关。结论　筛选克隆的新基因ＰＳＭＣＨ１ 在动脉粥样硬化斑块形成时可能有较重要的生物学作用。     

经皮射频微创治疗小肝癌

目的　总结经皮射频消融 (PRFA)治疗肿瘤直径 <5cm小肝癌的疗效和经验。方法　1999年 8月至 2 0 0 2年 6月期间 ,以根治为目的 ,采用PRFA治疗肿瘤直径≤ 5cm的单个原发性肝癌共 84例 ,2 2例结合经皮肝动脉栓塞化疗 ;35例同时行PRFA联合瘤内无水酒精注射术。结果　肿瘤≤ 3cm组 5 5例中第 1年出现肝内复发者 2例 ,死亡 1例 ;第 2年出现肝内复发者 2例 ,死亡 1例。肿瘤大小介于 3～ 5cm组 2 9例中 ,第 1年出现肝内复发者 3例 ,死亡 2例 ;第 2年出现肝内复发者 4例 ,死亡 3例。结论　PRFA为小肝癌治疗提供了一种新的手段 ,经过近 3年技术的提高和经验的积累 ,射频消融完全有可能杀灭肿瘤直径 <5cm的小肝癌 ,达到根治性效果。本组对 84例小肝癌进行以射频消融为主的非手术微创治疗 ,近期疗效满意 ,远期疗效有待进一步随访观察 ,射频消融对小肝癌治疗有着良好的前景。     

Greater Curvature Plication with Duodenal–Jejunal Bypass: a Novel Metabolic Surgery for Type 2 Diabetes Mellitus

Objective

The study investigated the use of great curvature plication with duodenal–jejunal bypass (GCP-DJB) in a type 2 diabetic with obesity rat model.

Methods

Twenty-two Sprague-Dawley rats were given a high fat and sugar diet with subsequent intraperitoneal injection of a small dosage of streptozotocin (30 mg/kg) and randomly assigned to either GCP-DJB (n?=?12) or Sham surgery (n?=?10). Body weight, peripheral blood glucose, and fasting serum insulin were assayed, and insulin resistance index (IRI) was calculated, before and at 1, 2, 4, and 8 weeks after surgery.

Results

No differences were found in the preoperative characteristics of the two groups (P?>?0.05). At week 1, the body weights decreased significantly, but there was no significant difference between the two groups (P?>?0.05).The fasting blood glucose was significantly lower in the GCP-DJB than in the Sham group (P?<?0.05), serum insulin levels were higher (P?<?0.05), and IRI began to decline (P?<?0.05). From 2 to 8 weeks, the body weight of Sham group gradually recovered and continued to rise, while the GCP-DJB group remained at a relatively lower state. Compared to the Sham group, the body weight, fasting blood glucose as well as IRI of GCP-DJB rats had significantly decreased (P?<?0.05). But, the fasting insulin concentrations had significantly increased (P?<?0.05).

Conclusion

This novel GCP-DJB procedure established a stable animal model for the study of metabolic surgery to treat type 2 diabetes mellitus (T2DM).

     

β 连环蛋白对脑胶质瘤辐射抗性的影响

目的 探讨β 连环蛋白（β-catenin）在X 射线照射条件下对人脑胶质瘤U87 细胞增殖、生存 的影响。方法 U87 细胞经采用5μmol/L IWR-1-endo 处理后,用X 射线照射,采用CCK-8 法平板克隆 形成实验检验细胞增殖和存活能力,免疫荧光染色检验细胞相关蛋白的表达,电镜观察细胞内微结构的变化。 结果 人脑胶质瘤U87 细胞辐射后,照射组β-catenin 蛋白相对表达量比对照组低（P <0.05）;经IWR-1- endo 处理的细胞增殖和平板克隆形成率有增加趋势（P <0.05）。结论 降低β-catenin 抑制Wnt/β-catenin 信号通路,虽然能降低胶质瘤细胞增殖能力,但可能通过保护细胞线粒体的方式增加胶质瘤细胞对辐射治疗 的抗性。