全文获取类型
收费全文 | 94904篇 |
免费 | 8254篇 |
国内免费 | 6245篇 |
专业分类
耳鼻咽喉 | 1035篇 |
儿科学 | 1015篇 |
妇产科学 | 750篇 |
基础医学 | 10393篇 |
口腔科学 | 1504篇 |
临床医学 | 10787篇 |
内科学 | 12834篇 |
皮肤病学 | 1006篇 |
神经病学 | 4198篇 |
特种医学 | 4217篇 |
外国民族医学 | 29篇 |
外科学 | 11706篇 |
综合类 | 17941篇 |
现状与发展 | 23篇 |
一般理论 | 7篇 |
预防医学 | 6932篇 |
眼科学 | 1960篇 |
药学 | 9716篇 |
86篇 | |
中国医学 | 5589篇 |
肿瘤学 | 7675篇 |
出版年
2024年 | 266篇 |
2023年 | 1152篇 |
2022年 | 3007篇 |
2021年 | 4091篇 |
2020年 | 3167篇 |
2019年 | 2589篇 |
2018年 | 2798篇 |
2017年 | 2733篇 |
2016年 | 2717篇 |
2015年 | 4234篇 |
2014年 | 5365篇 |
2013年 | 5438篇 |
2012年 | 7965篇 |
2011年 | 8359篇 |
2010年 | 6241篇 |
2009年 | 5281篇 |
2008年 | 6080篇 |
2007年 | 5984篇 |
2006年 | 5579篇 |
2005年 | 5023篇 |
2004年 | 3633篇 |
2003年 | 3509篇 |
2002年 | 3125篇 |
2001年 | 2329篇 |
2000年 | 1725篇 |
1999年 | 1362篇 |
1998年 | 898篇 |
1997年 | 861篇 |
1996年 | 674篇 |
1995年 | 568篇 |
1994年 | 454篇 |
1993年 | 269篇 |
1992年 | 325篇 |
1991年 | 274篇 |
1990年 | 215篇 |
1989年 | 196篇 |
1988年 | 202篇 |
1987年 | 150篇 |
1986年 | 133篇 |
1985年 | 105篇 |
1984年 | 54篇 |
1983年 | 51篇 |
1982年 | 44篇 |
1981年 | 29篇 |
1980年 | 18篇 |
1979年 | 31篇 |
1978年 | 16篇 |
1975年 | 11篇 |
1973年 | 10篇 |
1969年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
11.
鸡抗内毒素卵黄抗体IgY的制备 总被引:2,自引:1,他引:1
目的:研究分别应用内毒素(LPS)、类脂A(LipidA)和大肠杆菌突变株15免疫鸡后其蛋黄中抗内毒素抗体IgY的产量、纯度、效价并筛选最佳免疫抗原。方法:分别应用内毒素(LPS)、类脂A(LipidA)和大肠杆菌J5突变株作为抗原免疫25周龄Leghom鸡,水溶法(WD)提取蛋黄中抗体IgY,双紫外光测定抗体含量,SDS-PSGE电泳检测抗体纯度,细胞酶联染色和ELISA检测抗体特异性、效价及筛选最佳免疫抗原。结果:3种抗内毒素IgY含量和效价分别为14.4mg/ml和1:12 800(J5)、10.61mg/ml和1:12 800(LPS)、9.26mg/ml和1:3200(LipidA),抗体纯度均为95%左右。结论:大肠杆菌突变株J5和内毒素(LPS)为最佳免疫抗原,免疫鸡后其蛋黄中抗内毒素抗体IgY的产量和效价最高。 相似文献
12.
AClinicalStudyofReversingLeftVentricularHypertrophyinHypertensivePatientsbyAdalatZhangFumin(张馥敏)XuDi(许迪)YongYonghong(雍永宏)Chen... 相似文献
13.
The response of single detergent treated bovine retinal pigment epithelial cells in culture to ATP was measured with an image analyser. The most pronounced contraction was produced by 1.0 mM ATP with most change taking place in the first 10 min. At 1 h the area had decreased by about 33%, perimeter 22% and maximum length 25%. By way of comparison rabbit skin fibroblasts had a decreased area of approximately 40%, perimeter 25% and maximum length 22%. Bovine aortic smooth muscle cells on the other hand decreased in area by 55%, perimeter 40% and maximum length 36%. It is hoped that this assay may be used to evaluate drugs which could counteract contractile events in proliferative vitreoretinopathy. 相似文献
14.
Joseph M Dhahbi Shelley X Cao Patricia L Mote Brian C Rowley John E Wingo Stephen R Spindler 《The Journal of nutrition》2002,132(1):31-37
Molecular chaperones assist in the biosynthesis and processing of proteins. Most chaperones are induced by physiological stresses. We have shown that dietary energy restriction decreases the mRNA and protein levels of many endoplasmic reticulum chaperones in the livers of mice. Here, we have investigated the response of chaperone mRNA to feeding. Control and 50% energy-restricted C3B10RF1 mice were deprived of food for 24 h, fed, and killed 0, 1.5, 5 or 12 h after feeding. Chaperone mRNAs were strongly induced as early as 1.5 h after feeding in control and energy-restricted mice. The integrated levels of these mRNA over 24 h were significantly lower in energy-restricted mice. The mRNA response to energy intake was mirrored over the course of days in the level of chaperone protein. A similar but smaller response to feeding was found in kidney and muscle. Puromycin and cycloheximide failed to inhibit the feeding response, suggesting that feeding releases chaperone expression from an unstable inhibitor. Studies with dibutyryl-cAMP- and glucagon-supplemented, normal and streptozotocin-diabetic mice suggest that glucagon and insulin may be mediators of the feeding response. Adrenalectomy enhanced the feeding induction, but dexamethasone administration had no effect. Thus, postprandial changes in insulin and glucagon may link chaperone gene expression to feeding, possibly in several tissues including liver. 相似文献
15.
rhGM—CSF对小鼠口腔粘膜损伤的防治作用 总被引:2,自引:0,他引:2
目的:观察rhGM-CSF对TMX致实验性小鼠口腔粘膜损伤的疗效。方法:按随机分组原则将501只昆明种小白鼠分成8组,分别在相应时间给予不同药物(MTX,CF或rhGM-CSF)的处理因素,于第1-10天光镜下观察小鼠口腔粘膜的病理改变和积分情况。结果:IDMTX致口腔粘膜损伤病变率(45%-63%)和积分率(19.4%-56%)较其它组高,且死亡率很低(小于5%);IDMTX+GM0(或GM2)组的口腔粘膜损伤病变率(30.53%和30.99%)和积分率(19.47%和17.25%)比IDMTX组(55.56%和36.31%)明显减少,且溃疡严重程度较轻,二者相差显著(P<0.01)。结论:IDMTX致小鼠口腔粘膜损伤模型可以用于粘膜损伤的研究;rhGM-CSF可以减少MTX致小鼠口腔粘膜损伤,并促进粘膜损伤恢复。 相似文献
16.
17.
目的:观察喉罩全麻下行颈动脉狭窄的造影诊断及介入治疗术的临床效果。方法:择期DSA下颈动脉狭窄患者23例,年龄42-78岁,无明显肺部疾患及喉罩禁忌症患者,异丙酚(Pmpofol)泵入静脉全麻下插入喉罩完成手术,观察其不同时段的BP(MAP)、SpO2、HR、ECG(ST-Ⅱ)。结果:各时段的BP(MAP)、Sp02、HR、ECG(ST-Ⅱ)比较无显著性差异。结论:喉罩全麻在行颈动脉狭窄的造影诊断及介入治疗术的临床效果是肯定的。喉罩全麻颈动脉狭窄造影介入治疗 相似文献
18.
Chang Y Jung Hyo S Choi Jin S Ju Hyo S Park Tae G Kwon Yong C Bae Dong K Ahn 《The journal of pain》2006,7(10):747-756
The present study investigated the role of central metabotropic glutamate receptors (mGluRs) in interleukin-1beta (IL-1beta)-induced mechanical allodynia and mirror-image mechanical allodynia in the orofacial area. Experiments were carried out on male Sprague-Dawley rats weighing 230 to 280 g. After administration of 0.01, 0.1, 1, or 10 pg of IL-1beta into a subcutaneous area of the vibrissa pad, we examined the withdrawal behavioral responses produced by 10 successive trials of an air-puff ramp pressure applied ipsilaterally or contralaterally to the IL-1beta injection site. Subcutaneous injection of IL-1beta produced mechanical allodynia and mirror-image mechanical allodynia in the orofacial area. Intracisternal administration of CPCCOEt, a mGluR1 antagonist, or MPEP, a mGluR5 antagonist, reduced IL-1beta-induced mechanical allodynia and mirror-image mechanical allodynia. Intracisternal administration of APDC, a group II mGluR agonist, or L-AP4, a group III mGluR agonist, reduced both IL-1beta-induced mechanical allodynia and mirror-image mechanical allodynia. The antiallodynic effect, induced by APDC or L-AP4, was blocked by intracisternal pretreatment with LY341495, a group II mGluR antagonist, or CPPG, a group III mGluR antagonist. These results suggest that groups I, II, and III mGluRs differentially modulated IL-1beta-induced mechanical allodynia, as well as mirror-image mechanical allodynia, in the orofacial area. PERSPECTIVE: Central group I mGluR antagonists and groups II and III mGluR agonists modulate IL-1beta-induced mechanical allodynia and mirror-image mechanical allodynia in the orofacial area. Therefore, the central application of group I mGluR antagonists or groups II and III mGluR agonists might be of therapeutic value in treating pain disorder. 相似文献
19.
稳定性核素测定大鼠小肠蛋白质合成 总被引:2,自引:1,他引:1
目的:建立稳定性核素([L-^15N]亮氨酸)测定大鼠小肠蛋白质合成率的方法。方法:分别测定静脉注射相同剂量[L-^15N]亮氨酸不同时相的大鼠小肠^15N丰度及不同剂量[L-^15N]亮氨酸同一时相的大鼠小肠^15N丰度。结果:大鼠小肠游离氨基酸池中^15N核素丰度在注射后0.5h内呈线性上升并达高峰,维持4h后缓慢下降,小肠蛋白质中的^15N丰度0.5h至12h基本维持不变;随着注射剂量的增加,大鼠小肠蛋白质分数合成率(FSR)亦增加,当[L-^15N]亮氨酸剂量在1.0mmol/kg以上,FSR并不随施加[L15N]亮氨酸剂量的加大而增加。结论:在进行大鼠小肠蛋白质合成率测定时,一次性静脉注射的测量最佳时限为0.5h,剂量为1.0mmol/kg。 相似文献
20.