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David A. Amato Howard Bruckner DuPont Guerry IV Arlene Ash Geoffrey Falkson Ernest C. Borden Richard H. Creech Edwin D. Savlov Thomas J. Cunningham 《Investigational new drugs》1987,5(3):293-297
In this Eastern Cooperative Oncology Group (ECOG) phase II study, dibromodulcitol (DBD) and a combination of actinomycin D, hydroxyurea, and cyclophosphamide (AHC) were compared with methyl-CCNU, the current ECOG standard, in patients who had received no prior chemotherapy for disseminated malignant melanoma. The response rates were 6% (3/50) for AHC, 9% (3/34) for DBD, and 14% (7/49) for methyl-CCNU. Median survival times were 4, 5, and 6 months, respectively. Neither regimen appears to offer any advantage over methyl-CCNU as front-line therapy for patients with disseminated melanoma. 相似文献
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PCR with arbitrary primers: approach with care 总被引:13,自引:0,他引:13
W. C. Black IV 《Insect molecular biology》1993,2(1):1-6
New techniques have recently been described that employ the polymerase chain reaction (PCR) to amplify arbitrary regions of a genome using a single primer. The techniques reveal polymorphisms in insect taxa that lack allozyme variation and, for the first time, permit genetic polymorphisms to be rapidly analysed in small arthropods (e.g. mites, endoparasitic wasps). The methods have been used in identification of sub-species and cryptic species, and have applications in population genetics and genetic fingerprinting. They are fairly inexpensive, do not require the use of radioactivity, are relatively simple to learn and can easily be adapted to most laboratories. However, their application is not without technical problems and practical limitations. The purpose of this note is to indicate the critical factors to consider before launching into their use. We chiefly emphasize that most polymorphisms revealed by these methods segregate as dominant markers. Furthermore, application of these techniques requires extensive standardization and may not prove to be reproducible among various laboratories especially those employing different types of thermal cyclers. There are some unique features of these polymorphisms to consider when using them in genetic fingerprinting. In addition, because the techniques amplify arbitrary regions of genomes, similarly sized fragments amplified between two species may not be homologous. This argument and empirical observations suggest that PCR with arbitrary primers will have limited application in molecular systematics above the intraspecific level. 相似文献
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Kathy Murray MD Consulting Radiologist Adjunct Associate Professor of Radiology Marc Gosselin MD Associate Professor of Radiology Mark Anderson MD Radiology Resident Grant Berges Medical Student IV Eric Bachman MD Radiology Resident 《Seminars in Ultrasound, CT and MRI》2002,23(4):352-371
Radiologists rely on imaging patterns to arrive at a diagnosis. The different morphological patterns in the lungs are well known, but less emphasis has traditionally been placed on the pattern of distribution. This important feature greatly assists in the differential diagnosis regarding many pulmonary diseases and is the focus of this article. Chest radiographs often result in a narrow differential if one understands the regional differences and microenvironments within the lung and takes into consideration the ancillary imaging findings. High-resolution computed tomography offers additional information at the level of the secondary pulmonary lobule to fine-tune the distribution pattern and, consequently, the differential diagnosis. Disease distribution is often as important as the morphologic appearance of the disorder. This article will approach pulmonary diseases from the perspective of distribution patterns, highlighting the more common patterns. The goal of this review article is to give radiologists a conceptual framework that may be applied in their daily work environment. 相似文献
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Serologic screening of United States blood donors for Babesia microti using an investigational enzyme immunoassay 下载免费PDF全文
Andrew E. Levin Phillip C. Williamson Evan M. Bloch Joan Clifford Sherri Cyrus Beth H. Shaz Debra Kessler Jed Gorlin James L. Erwin Neil X. Krueger Greg V. Williams Oksana Penezina Sam R. Telford IV John A. Branda Peter J. Krause Gary P. Wormser Anna M. Schotthoefer Thomas R. Fritsche Michael P. Busch 《Transfusion》2016,56(7):1866-1874
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Ponnila S Samuel Jane P Pringle Nathaniel W James IV Susan J Fielding Kathleen M Fairfield 《International journal for equity in health》2009,8(1):30-5