Effectiveness and safety of reduced-port laparoscopic surgery vs conventional multi-port laparoscopic surgery in the treatment of gastric diseases: A meta-analysis

This study aimed to compare the effectiveness and safety of reduced-port laparoscopic surgery (RPLS) and conventional multi-port laparoscopic (CMPLS) surgery in the treatment of gastric diseases.The PubMed, Embase, Cochrane Library, Web of Science, and Chinese Biomedical Literature databases were systematically searched for randomized controlled trials, cohort studies, and case control studies on the use of RPLS vs conventional multi-port laparoscopic surgery in treating gastric diseases from their inception until March 10, 2019. The evaluated outcomes were the operative time, blood loss, length of hospital stay, number of dissected lymph nodes, postoperative complications, and conversions. All of these were compared using Stata software version 12.0.A total of 18 studies were included, which involved 2938 patients. In studies referring to the comparison between RPLS and CMPLS in treating gastric diseases, the former showed significantly inferior in terms of operative time (P = .011) and number of dissected lymph nodes (P = .031); but superior results in terms of the estimated blood loss (P = .000) and length of hospital stay (P = .001) than the latter did; however, the rates of postoperative complications (P = .830) and conversions (P = .102) were not statistically significant between the 2 groups.RPLS and CMPLS showed comparable effectiveness and safety in the treatment of gastric diseases in our meta-analysis. Based on the current evidence, we believe that RPLS is an efficacious surgical alternative to CMPLS in the management of gastric diseases because of the shorter hospital stay and reduced blood loss. However, large-scale, well-designed, multicenter studies are needed to further confirm the results of this study.     

Learning and memory impairment induced by 1,4-butanediol is regulated by ERK1/2-CREB-BDNF signaling pathways in PC12 cells

Metabolic Brain Disease - 1,4-butanediol (1,4-BD) is a known γ-hydroxybutyric acid (GHB) precursor which affects the nervous system after ingestion, leading to uncontrolled behavioral...     

10月龄APP/PS1双转基因AD小鼠海马超微结构特征的观察

目的:观察APP/PS1双转基因小鼠海马内Aβ沉积的形态特征。方法:以10月龄雄性APP/PS1双转基因小鼠和C57BL/6小鼠为研究对象,采用Aβ免疫组化和透射电镜技术,观察海马部位Aβ的沉积情况及相关的病理特征。结果:在双转基因小鼠海马内存在致密性神经炎性斑块及营养障碍性突起,但脑微血管壁的Aβ沉积不甚明显;营养障碍性突起及神经细胞、微血管内皮细胞、周细胞内存在大量的自噬泡及次级溶酶体,提示该模型存在自噬溶酶体途径功能紊乱。结论:该鼠模拟了AD的老年斑及相关退行性变等病理改变,其中可能伴有细胞多种代谢的变化及血脑屏障功能的变化。从老年斑角度而言,该动物是成功的阿尔茨海默病动物模型,可用于AD的生化、病理研究及新治疗方法的探索。     

Historically‐informed nursing: A transnational case study in China

The term ‘nurse’ (hushi—’caring scholar’) did not enter the Chinese language until the early 20th century. Modern nursing—a fundamentally Western notion popularized by Nightingale and introduced to China in 1884—profoundly changed the way care of the sick was practiced. For 65 years, until 1949, nursing developed in China as a transnational project, with Western and Chinese influences shaping the profession of nursing in ways that linger today. Co‐authored by Chinese, Canadian, and American nurses, this paper examines the early stages of nursing in one province of China as an exemplar of the transnational nature of nursing development. By identifying sociopolitical influences on the early development of nursing in Shandong, the authors aimed not only to contribute to the nascent body of knowledge on China nursing history, but also to heighten readers’ sensitivity to the existence of historical echoes, residue, and resonances in their own nursing practices. Tracing current issues, values, or practices back to their roots provides context and helps us to better understand the present. Whether we are aware of the details or not, the gestalt of nursing practice in a particular place has been shaped by its history—including in Shandong province in China.     

计算机辅助设计与制作技术在眼眶爆裂性骨折治疗中的应用及疗效评价

背景 眼眶爆裂性骨折引起明显的眼球形态和功能的异常.眼眶解剖结构复杂,故眼眶骨折修复手术中植入物的设计和制作具有一定的难度,而计算机辅助设计与制作(CADM)技术有望提供一种新的方法,但其临床应用价值有待评估.目的 研究CADM技术在眼眶爆裂性骨折治疗中的应用方法,评价其临床效果.方法 对河南省眼科研究所河南省立眼科医院2006年7月至2012年7月收治的眼眶爆裂性骨折患者74例74眼的手术过程和疗效进行回顾性分析,其中CADM手术者58例58眼,非CADM手术者16例16眼,2个组间患者基线特征,如年龄、性别和眼别均匹配.CADM手术组术眼采用CADM技术进行个体化三维立体植入物的设计和制作并进行眶骨骨折缺损处的填充,非CADM手术组术眼采用常规羟基磷灰石人工骨或多孔聚乙烯高分子合成材料(Medpor)填充法.所有术眼术前均行常规眼部检查以及CT检查,术后随访22～69个月,在随访期间内定期进行眼部临床观察和CT检查.观察指标包括最佳矫正视力、眼球突出度、眼球位置、眼球运动和复视改善情况以及并发症情况.结果 CADM手术组和非CADM手术组术眼术前最佳矫正视力分别为0.71±0.37和0.69±0.41,术后最佳矫正视力分别为0.74±0.38和0.72±0.41,组间差异无统计学意义(F分组=0.043,P=0.837),术眼手术前后视力的总体比较差异有统计学意义(F时间=13.576,P＜0.01),其中2个组术眼术后视力均好于术前,差异均有统计学意义(均P＜0.05).2个组间复视和眼球运动障碍治愈和好转的眼数分布差异均无统计学意义(Z=-0.298,P=0.766;Z=-0.548,P=0.584).CADM手术组和非CADM手术组术眼术前眼球眼球内陷度值分别为(3.93±0.99) mm和(3.88±0.97)mm,术后分别为(0.91±0.67)mm和(1.84±0.80)mm,组间总体比较差异无统计学意义(F分组=3.558,P=0.063),各组术眼术后眼球眼球内陷度值均明显低于术前,差异均有统计学意义(均P＜0.05).CT影像显示,CADM手术组术眼植入物在位,与眶骨骨折缺损处贴合良好,患者眼球位置及外观均恢复正常.非CADM手术组术眼发生植入物翘起并损伤内直肌.随访期内CADM手术组未发现组织感染、排斥反应等不良反应.结论 CADM技术用于眼眶爆裂性骨折的治疗可重建三维立体骨性眼眶,并有效改善患眼位置、眼球运动功能和视觉功能,其疗效优于传统植入物填充术.     

The effect of Fe2O3 and ZnO nanoparticles on cytotoxicity and glucose metabolism in lung epithelial cells

Metallic nanoparticles (NPs) have potential applications in industry and medicine, but they also have the potential to cause many chronic pulmonary diseases. Mechanisms for their cytotoxicity, glucose and energy metabolism responses need to be fully explained in lung epithelial cells after treatment with metallic nanoparticles. In our study, two different metallic nanoparticles (Fe₂O₃ and ZnO) and two cell‐based assays (BEAS‐2B and A549 cell lines) were used. Our findings demonstrate that ZnO nanoparticles, but not Fe₂O₃ nanoparticles, induce cell cycle arrest, cell apoptosis, reactive oxygen species (ROS) production, mitochondrial dysfunction and glucose metabolism perturbation, which are responsible for cytotoxicity. These results also suggest that the glucose metabolism and bioenergetics had a great potential in evaluating the cytotoxicity and thus were very helpful in understanding their underlying molecular mechanisms. Copyright © 2015 John Wiley & Sons, Ltd.     

大鼠体内大脏器创伤模型涂布康派特?医用胶后生物反应观测

目的：评价康派特?医用胶对大鼠内脏创面止血后的安全性及其体内降解情况。方法：大鼠随机分为空白对照组,手术对照组和手术涂胶组,给胶组分别给予8μL或40μL康派特?医用胶。术后动物进行临床症状观察,体重、摄食量测定,血常规、血清生化学、脏器重量和组织病理学检查。结果：各项指标均未见与康派特?医用胶相关毒性反应;6个月组织病理学检查可见胶体被纤维结缔组织逐渐分割,多核巨细胞和炎症细胞参与异物降解。结论本实验条件下,未见康派特?医用胶对大鼠机体有毒性作用,胶体在大鼠体内6个月开始降解。     

MicroRNAs in hypertrophic cardiomyopathy: pathogenesis,diagnosis, treatment potential and roles as clinical biomarkers

Heart Failure Reviews - Hypertrophic cardiomyopathy (HCM) is the most common heritable cardiomyopathy and is characterized by increased left ventricular wall thickness, but existing diagnostic and...