X连锁无汗性外胚叶发育不全家系ED1基因的突变检测

目的探讨国内X连锁无汗性外胚叶发育不全家系中ED1基因的突变情况，为该病的遗传咨询、产前诊断、确诊携带者提供依据。方法收集2个X连锁无汗性外胚叶发育不全家系及1个散发患者的外周血样本，盐析法提取基因组DNA，采用聚合酶链反应（polymerase chain reaction，PCR）和直接测序对ED1基因进行突变检测。结果家系一患者ED1基因第9外显子发生错义突变（1045G〉A），家系二和散发患者ED1基因第3外显子发生错义突变，分别为467G〉A和466C〉T。结论ED1基因的错义突变可导致X连锁无汗性外胚叶发育不全。这3个突变与国外学者的报道一致。     

雷帕霉素联合顺铂对CCL19调节头颈鳞状细胞癌细胞活性的影响

目的 探讨雷帕霉素(rapamycin)与顺铂(cisplatin)联合使用对趋化因子CCL19调节头颈鳞状细胞癌细胞活性的影响.方法 应用甲基噻唑基四唑(MTT)法及流式细胞仪检测顺铂及雷帕霉素对CCL19诱导后人头颈鳞状细胞癌淋巴结转移细胞系(PCI-4B、PCI-37B)生长抑制作用、细胞凋亡作用和细胞周期变化.使用Calcusyn软件计算两种药物(浓度比为80:1)联合使用时的剂量效应参数及联合指数(combination index,CI),使用SPSS 11.0软件包进行统计分析.结果 顺铂及雷帕霉素可以分别提高CCL19作用后细胞的生长抑制率(CCL19+顺铂作用后两细胞系的生长抑制率为21.22%±2.68%和22.76%±2.79%,CCL19+雷帕霉素作用后为19.46%±2.54%和20.6%±4.38%)、细胞G1期比例(CCL19+顺铂作用后两细胞系的G1期比例为72.14%±2.66%和76.39%±1.45%,CCL19+雷帕霉素作用后为75.26%±5.92%和74.00%±1.51%)和凋亡率(CCL19+顺铂作用后两细胞系的凋亡率9.59%±0.86%和7.55%±0.66%,CCL19+雷帕霉素作用后为8.21±1.80%和6.26±0.78%).两种药物浓度比为80:1联合使用时,PCI-4B细胞在半数抑制量(inhibitory concentration 50%,IC50)以下,CI＜1,IC75以上CI＞1;PCI-37B则在IC75以下CI＜1,IC90以上CI＞1.结论 顺铂及雷帕霉素均有抑制CCL19对人头颈鳞状细胞癌淋巴结转移细胞的活性调节作用,并且二者联合使用存在协同作用.

Abstract：

Objective To investigate the synergistic effects of rapamycin and cisplatin on head and neck squamous cancer cells regulated by chemokine( C-C motif) ligand 19 (CCL19). Methods The role of rapamycin and cisplatin was detected on cell-cycle and apoptosis in CCL19 induced PCI-4B and PCI-37B cells by methyl thiazolyl tetrazolium (MTT) and flow cytometry(FCM). Dose-effect relationship parameters and combination index(CI) were calculated on the median-effect equation and multiple drug effect equation using computer software CalcuSyn. Statistical analysis was performed by the unpaired student's t-test.Results Rapamycin and cisplatin could respectively increase the growth arrest, the proportion of G1 phase and apoptosis of CCL19 induced cancer cells (P ＜0. 05 ). Under inhibitory concentration 50% (IC50), CI was less than 1, and in IC75, it was more than 1 in PCI-4B cells. In PCI-37B cells, under IC75, CI was less than 1, and in IC90, it was more than 1. Conclusions Rapamycin and cisplatin can inhibit CCL19-regulated PCI-4B and PCI-37B cells' survival. The two drugs have synergistic effects when used in combination.     

Relationship between vitamin D receptor gene polymorphism and periodontitis

Recent studies have shown that vitamin D receptor (VDR) gene polymorphism had regulatory effects on bone mineral density (BMD) and bone turnover. The VDR gene has also been indicated as a candidate gene for the susceptibility of osteoporosis. However, it is unclear whether VDR genotypes could be associated with alveolar bone loss of patients with periodontitis, or whether vitamin D receptor gene could be a candidate gene for susceptibility to periodontitis. The purpose of this study was to answer these two questions. METHODS: Twenty-four cases of adult periodontitis (AP), 37 cases of early onset periodontitis (EOP) and 39 healthy controls were recruited for the study. Individual samples of venous blood and DNA were obtained from each subject. Genotypes of the TaqI VDR gene were determined by PCR and TaqI restriction endonuclease digestion. RESULTS: One out of 24 AP patients, nine out of 37 EOP patients and two out of 39 healthy controls were detected with Tt genotype, while the rest had the TT genotype. The detected frequency of Tt genotype was significantly higher in EOP patients (24.3%) than in AP patients (4.2%) and healthy controls (5.1%). The frequency of t allele was also significantly higher in EOP patients. There was no statistical difference in the distribution of TaqI VDR genotypes between AP patients and healthy controls. The study suggests that Tt genotype might be a risk indicator for the susceptibility to EOP. Carriage of the allele (t) of the TaqI VDR gene may increase the risk of developing EOP.     

根管预备并发症及充填质量的临床评价

目的：对根管预备并发症及充填质量进行临床评价。方法：从Digora图像系统中抽取我科2002—04—05所完成的根管治疗病例805例，分别对前牙、前磨牙和磨牙根管预备并发症的发生和充填质量进行分析。结果：形成台阶和侧穿的比例在前磨牙分别为4％，而在磨牙中则分别为33％和5％。前牙、前磨牙和磨牙适充的比例分别为74％、69％和50％。结论：磨牙根管预备和充填的质量仍有待进一步提高。     

A comparative morphologic study of Carabelli cusp between Chinese and Japanese students.]

The study of teeth,dentition and occlusion is helpful to guide oral medical clinic and the study of anthropology.Our department of Osaka Dental University and had many items of research work about teeth,dentition and occlusion between Chinese and Japanese students.This article reports the study of Carabelli cusp.The results revealed:(1)the rate of Carabelli cusp is higher in Japanese than in Chinese and is higher in male than in female.(2)the rate of Carabelli cusp of the first maxillary molar of both side is very high.(3)The rate of Carabelli cusp is related to the height and the type of cusp numbers.These all illustrated the occurence of Carabeiil cusp were influenced by race, individual and the development of teeth.     

固齿健周煎剂对老年小鼠的抗氧化作用

目的探讨中药固齿健周煎剂对老年小鼠的抗氧化作用。方法实验组为固齿健周煎剂灌胃的老年小鼠，对照组为灌胃等量自来水的青、老年小鼠。检测过氧化脂质、超氧化物歧化酶及过氧化氢酶。结果实验组与青、老年小鼠对照组比较差异均有显著性。结论固齿健周煎剂具有抗脂质过氧化的作用，可能对调节机体代谢功能、抗自由基损伤及恢复牙周组织健康有益     

Clinical application of angiography of oral and maxillofacial hemangiomas. Clinical analysis of seventy cases

Angiographic findings in seventy cases of maxillofacial hemangioma are reported. The tumors have been divided into four types: (1) plexiform hemangioma, (2) cavernous hemangioma with venous drainage, (3) cavernous hemangioma without venous drainage, and (4) the so-called "diffuse type" of cavernous hemangioma. The angiographic features of the four types are described, and clinical application is evaluated. The angiograms demonstrate the location, extent, blood supply, and drainage of the tumors and show whether they communicate intracranially, thus providing valuable criteria for planning and determining the mode of treatment.     

矿物三氧化物凝聚体用于犬牙直接盖髓的实验研究

目的：观察矿物三氧化物凝聚体(MTA)直接盖髓后牙髓炎症反应和修复性牙本质的形成。方法：人工机械暴露犬牙髓，用MTA或氢氧化钙直接盖髓。结果：实验2周和8周，MTA诱导修复性牙本质形成的效果优于氢氧化钙，炎症反应较轻。结论：MTA是一种效果较好的盖髓剂。     

3172个根管充填质量的临床评价

目的　通过数字化X线图像 ,分析武汉大学口腔医学院牙体牙髓科临床医师和进修医师根管充填的质量及继续教育的效果。方法　将 6名长期从事临床教学的临床医师 (A组 )和 8名进修医师 (B组 )分为两组 ,这两组医师在 2 0 0 3年 4～ 9月间随机完成根管治疗 2 0 4 3颗牙 ( 3172个根管 ) ,对两组根管充填质量进行分析和比较。结果　患牙根管治疗总体适充率为 4 9 6 % ,A组患牙和根管适充率分别为 5 9 9%和 6 3 9% ,明显高于B组的 4 0 1%和 4 7 4 % ,差异有显著性 (P <0 0 0 1)。B组前 2个月根管适充率为 4 0 0 % ,后 2个月提高为 5 7 8% ,差异有显著性 (P <0 0 0 1) ,且后 2个月根管适充率在前磨牙和磨牙与A组接近 ,差异无显著性 (P >0 0 5 )。结论　A组根管充填质量较好 ;B组在半年内根管充填质量有显著提高