Treatment of retrograde ejaculation with imiprimine

We describe 2 cases of retrograde ejaculation in diabetic patients. Treatment with the alpha-adrenergic stimulator, imiprimine, resulted in reversal of seminal trajectory.     

Molecular forms of acid brain proteinases

Acid proteinases of the brain were isolated by gel filtration on a column with Sephadex G-100. Three peaks of enzyme activity were found. Cathepsin D with high molecular weight (over 150,000) is solubilized from brain tissues by 0.1% Triton X-100. Cathepsin D with a molecular weight of 59,000 is solubilized only by a 0.2% solution of the detergent. Low-molecular-weight (20,000) cathepsin D is a soluble cytoplasmic enzyme. The results are regarded as proof of the heterogeneity of molecular forms of acid proteinases in the cerebral cortex, corresponding to the strength of the bond between the enzymes and subcellular structure of brain tissue.Department of Biochemistry and Biophysics, Dnepropetrovsk University. (Presented by Academician of the Academy of Medical Sciences of the USSR S. E. Severin.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 80, No. 10, pp. 43–46, October, 1975.     

Conditions for efficient antibody binding with proteins of the human immunodeficiency virus studied by immunoblotting

The authors have investigated the antibody binding with individual HIV protein by "western" blotting method. The optimal condition for binding was incubation of nitrocellulose strips at 37 degrees C for 2 hrs followed by incubation at 4 degrees C overnight. Differences in the serologic activities of a number of sera in "western" blotting were shown by using two different antigens. It was concluded that mixing of different antigens before electrophoresis may be useful for more effective "western" blotting analysis.