经尿道前列腺汽化电切术治疗高危前列腺增生症58例报告

目的 探讨高危前列腺增生症(BPH)的有效手术新方法。方法 采用经尿道前列腺汽化电切术治疗高危BPH58例。结果 术后随访3～6个月，IPSS评分平均8．3分，最大尿流率平均15．2ml/s，残余尿量平均25ml，未出现严重并发症，疗效满意。结论 汽化和电切结合治疗高危前列腺增生症是一种安全性高、并发症少、疗效确切的手术方法。     

云南黄芪药用植物物种多样性研究

云南作为黄芪属植物的一个分布中心，具有丰富的药用植物资源，有正品黄芪２个种，代用品５个种和地方习用品１３个种，正品沙苑子１个种，代用品和地方用品各１种，本文从物种多样性方面对该药物植物进行讨论，并对其亚属，组的等级，特别是同且中的药用植物进行了研究，找出了一定规律。还介绍了几个兄弟民族对黄芪属药用植物的利用情况。     

Inhibition of bladder activity by 5-hydroxytryptamine1 serotonin receptor agonists in cats with chronic spinal cord injury

The serotonin (5-hydroxytryptamine1A) 5-HT1A receptor agonist 8-OH-DPAT [(R)- (+)-8-hydroxy-2-(di-n-propylamino)tetralin] inhibits bladder activity under nociceptive but not innocuous conditions in cats with an intact spinal cord, suggestive of an effect on primary afferent C fibers or their targets. Because C fibers play a key role in reflex micturition in chronic spinal cord injury (SCI), we investigated the effect of 8-OH-DPAT on micturition in SCI cats. We also investigated GR-46611 (3-[3-(2-dimethylaminoethyl)-1H-indol-5-yl]-N-(4-methoxybenzyl)acrylamide), which has agonist activity predominantly at 5-HT1B and 5-HT1D receptors but also at the 5-HT1A receptor. Chloralose-anesthetized cats were catheterized through the bladder dome for saline-filling cystometry. Dose-response curves for i.v. 8-OH-DPAT (0.3-30 microg/kg) and GR-46611 (0.03-300 microg/kg) were followed in three cases each by 5-HT1A antagonist WAY-100635 [N-tert-butyl-3-(4-(2-methoxyphenyl)-piperazin-1-yl)-2-phenylpropanamide] at 300 microg/kg. Threshold volume, capacity, residual volume, micturition volume, and arterial pressure were measured. Intact cats showed few significant changes in cystometric variables. SCI cats responded to both 8-OH-DPAT and GR-46611 with dose-dependent increases in threshold volume, capacity, and residual volume, significant at > or =10 microg/kg for 8-OH-DPAT and at > or =3 microg/kg for GR-46611. Effects of 8-OH-DPAT but not GR-46611 were largely reversed by WAY-100635. Both 5-HT1A and 5-HT1B/1D agonists may offer a promising means of reducing bladder hyperactivity and increasing bladder capacity in patients with chronic SCI.     

Food intake,survival, and immunity of Nibea albiflora to Cryptocaryon irritans infection

Parasitology Research - Cultured Nibea albiflora rarely die from cryptocaryoniasis. To explore the resistance of N. albiflora against the invasion of Cryptocaryon irritans, in this study,...     

Prevalence and Characterization of Somatic Mutations in Chinese Aldosterone-Producing Adenoma Patients

Recently somatic mutations of KCNJ5, ATP1A1, ATP2B3, and CACNA1D have been identified in patients with aldosterone-producing adenoma (APA). The present study sequenced the DNA in the tissues and blood samples from Chinese patients with APA for KCNJ5, ATP1A1, ATP2B3, and CACNA1D gene mutations.Among the 114 patients, 86 (75.4%) were identified with KCNJ5 somatic mutations, including 3 previously reported (G151R, L168R, T158A) and 2 other unreported mutations. One patient presented with both a point mutation (E147) and an insertion mutation, whereas another had a 36-base duplication, G153_G164dup. No mutation of ATP1A1 and ATP2B3 in the known hotspots was identified and only 1 male patient was detected with a novel CACNA1D mutation, V748I. Unlike other studies, male and female patients had similar KCNJ5 mutation rates (76.9% vs 74.2%). Mutation carriers were younger and had lower preoperative potassium level, whereas male (but not female) mutation carriers had higher preoperative plasma aldosterone concentration and preoperative blood pressures. Mutation carriers also had higher LV mass index (LVMI) than nonmutation carriers. After surgery, LVMI improved significantly in the KCNJ5 mutation group but not in the nonmutation group. The mRNA expression of KCNJ5, CYP11B2, and ATP2B3 was higher in the KCNJ5-mutated APA tissues. Functional characterization of the 2 novel KCNJ5 mutations showed that they were associated with decreased proliferation, membrane depolarization, elevated secretion of aldosterone, and increased expression of CYP11B1 and CYP11B2.In conclusion, Chinese APA patients appear to have a high frequency of somatic KCNJ5 mutation. Mutation prevalence rates are similar among men and women and 2 novel mutations are identified. KCNJ5-mutated patients benefit more from surgical resection of APA than nonmutated patients.     

Numb 基因负性调节 CXCR4表达抑制膀胱癌细胞 BIU-87的增殖和迁移

目的：研究 Numb 基因对趋化因子受体4（CXCR4）表达的影响以及对膀胱癌细胞增殖和迁移的影响。方法实验分为3组：实验组（在膀胱癌细胞 BIU-87中转染 Numb-ORF 表达质粒）、阴性对照组（转染空载体）和空白对照组（未行转染处理）。使用荧光定量 PCR 和 Western blotting 检测各组细胞 Numb 和 CXCR4的表达。使用 MTS 法和 Transwell 小室法检测细胞增殖和迁移能力。结果实验组、阴性对照组和空白对照组中 Numb 基因表达水平分别为31.044±3.350、4.401±0.567和4.287±0.341,差异有统计学意义（F ＝183.418,P ＝0.000）;实验组、阴性对照组和空白对照组的 CXCR4表达水平分别为0.344±0.167、0.996±0.148和1.010±0.106,差异有统计学意义（ F ＝21.355,P ＝0.002）。细胞增殖检测中实验组、阴性对照组和空白对照组的48 h 吸光度值分别为0.615±0.057、0.987±0.063和0.957±0.066,差异有统计学意义（F ＝33.210,P ＝0.001）。迁移实验中,实验组、阴性对照组和空白对照组的穿膜细胞数分别为（164.667±19.858）个、（670.133±38.760）个和（667.533±27.610）个,差异有统计学意义（F ＝286.788,P ＝0.000）。结论 Numb 可能通过负性调节CXCR4表达抑制膀胱癌细胞的增殖和迁移。     

异位支气管囊肿的CT、MRI表现(附19例报告并文献复习)

目的探讨异位支气管囊肿的CT及MRI表现。资料与方法回顾性分析19例经手术及病理证实的异位支气管囊肿患者资料,讨论并复习其CT及MRI表现。结果椎管内8例,颈部3例,颅内3例,皮下组织2例,左侧肾上腺区、腹膜后及蝶窦内各1例。病变多为类圆形及椭圆形,偶有分叶,边界清楚,在CT像上呈较高密度影或囊性密度影,在MRI上呈长T1、长T2信号或双高信号,均无强化。异位支气管囊肿可继发感染并伴有先天畸形或肿瘤。结论异位支气管囊肿发病部位及临床表现缺乏特异性,CT及MRI有助于病变定位,明确病变性质,但要发现其支气管来源需依靠病理。     

Elementary studies on elevated steroidogenic factor-1 expression in aldosterone-producing adenoma

ObjectivesThe expression of steroidogenic factor-1 (SF-1) was elevated in adrenal aldosterone-producing adenoma (APA). The influence of SF-1 on adrenal tumorigenesis by adrenocortical cell line H295R cells was investigated.Materials and methodsReal-time PCR and Western blotting were used to detect SF-1 expression in 16 APA samples and 12 normal adrenal samples. Specific SF-1-shRNA plasmid was transfected into H295R cells to inhibit SF-1 expression. Western blotting and real-time PCR were used to verify the effects of RNAi on SF-1 inhibition. Subsequently, WST-1 and cell count were applied to evaluate cell proliferation at different SF-1 levels. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was used to measure cell apoptosis, and proliferation marker Ki-67 was studied by immunohistochemistry.ResultsCompared with normal adrenal samples, SF-1 mRNA and protein levels in APA samples were significantly higher. It was 10.48:1 at SF-1 mRNA and 0.87 ± 0.05 vs. 0.39 ± 0.07 at protein levels, respectively (P < 0.01). A decreased SF-1 significantly inhibited cell proliferation in the experimental and control cells. These results were supported by weaker Ki-67 staining in SF-1-inhibited cells [(36.9% ± 4.17%) vs. (58.48% ± 7.16%) (P < 0.01)]. Moreover, SF-1 inhibition induced a 2.7-fold increase in the percentage of apoptotic H295R cells (P < 0.01).ConclusionsElevated SF-1 may play an important role in APA formation and primary aldosteronism. SF-1 acts as an oncogenic factor, and its inhibition provides new insight into the understanding and treatment of related adrenal diseases.     

松质骨基质复合生物蛋白胶构建组织工程软骨的研究

目的尝试采用松质骨基质与生物蛋白胶复合材料构建组织工程软骨。方法体外培养大鼠软骨细胞,接种于松质骨基质／生物蛋白胶材料上行体外培养。采用HE、甲苯胺蓝染色免疫学检测、扫描电镜观察等方法观察所构建的组织工程软骨的特性。结果松质骨基质／生物蛋白胶组的组织学结构更接近于软骨样组织．其Ⅱ型胶原、蛋白多糖基因表达量及蛋白多糖含量明显高于松质骨基质组。结论松质骨基质／生物蛋白胶复合材料可用于构建组织工程软骨,是一种较理想的支架材料。