内皮细胞生长因子的分离和纯化

参照 Lobb 和 Fett 方法,用肝素亲和层析法从牛脑组织中提取出纯度较高的内皮细胞生长因子,后者能明显促进体外人脐静脉内皮细胞增殖。     

Essential Role of Sphingosine-1-Phosphate Receptor 1-Bearing CD8+CD44+CCR7+ T Cells in Acute Skin Allograft Rejection

A subset of naturally formed sphingosine‐1‐phosphate receptor 1 (S1P1)‐bearing CD8⁺CD44⁺CCR7⁺ memory T cells has been identified in transplant recipient BALB/c (H‐2^d) mice. The frequency of this subset of memory T cells is significantly increased in the spleen, lymph nodes and skin grafts in the recipient BALB/c mice during acute skin allograft rejections. The immune‐reconstitution with CD8⁺CD44⁺CCR7⁺S1P1⁺ memory T cells facilitates acute skin allograft rejection in SCID mice. Being Th1‐polarized and cytotoxic, CD8⁺CD44⁺CCR7⁺S1P1⁺ memory T cells proliferate and differentiate immediately into effectors upon encountering allo‐antigens. A siRNA against S1P1 inhibits CD8⁺CD44⁺CCR7⁺S1P1⁺ memory T cell‐mediated acute skin allograft rejection in SCID mice by means of knocking‐down S1P1‐expression. CCL21 mutant (CCL21‐ΔCT) has been used to compete with wild‐type CCL21 in the course of binding to CCR7. Combined administration of siRNA S1P1 and CCL21‐ΔCT significantly prolongs the survival of skin allograft in the recipient BALB/c mice by means of inhibiting accumulation of CD8⁺CD44⁺CCR7⁺S1P1⁺ memory T cells in the spleen and the skin grafts. Our data provide direct evidence that S1P1 and CCR7 are involved in the proliferation and trafficking of CD8⁺CD44⁺CCR7⁺S1P1⁺ memory T cells. S1P1 may serve as a functional marker for CD8⁺CD44⁺CCR7⁺ memory T cells. Targeting CD8⁺CD44⁺CCR7⁺S1P1⁺ T cells may be a useful strategy to prolong the survival of allograft transplant.     

过敏性皮肤病患者血清sELAM-1水平测定

目的：分析血清中可溶性内皮细胞－白细胞粘附分子（sELAM-1）含量变化与过敏性皮肤病发病的关系。方法：采用ELISA双抗体夹心法测定22例荨麻疹患，14例接触性皮炎患及36例湿疹患血清中sELAM－1含量与正常对照组相差不显（P＞0．05）；（2）接触性皮炎患血清中sELAM-1水平高于正常对照组（P＜0．05）；（3）湿疹患血清中sELAM-1水平明显高于正常对照组（P＜0．01）。皮损炎症程度重及皮损面积大明显高于皮损炎症程度轻皮损面积小（P＜0．01）。结论：接触性皮炎及湿疹患血清sELAM-1水平升高与疾病皮损炎症发生与发展有关，并与皮损炎症程度及皮损受累面积呈正相关。     

胃癌患者红细胞C3b受体与自然杀伤细胞活性的相关性研究

为研究胃癌患者的红细胞Ｃ３ｂ受体（ＲＢＣ－Ｃ３ｂＲ）与自然杀伤（ＮＫ）细胞活性之间的关系，利用酵母菌 环试验及ＭＴＴ比色法对３０例胃患者有３８例健康对照者的ＲＢＣ－Ｃ３ｂ受体及ＮＫ细胞活怀进行了测定。结果表明胃癌患者的这两项免疫指标均比正常对照显著低下（Ｐ〈０．０１）。经统计学相关性分析显示，这两项免疫指标间呈明显的正相关（ｒ＝０．６０７，Ｐ〈０．００１）。     

KTP激光汽化术治疗高龄高危良性前列腺增生的临床观察

目的探讨高能磷酸钛氧钾晶体(KTP)激光前列腺汽化术(PVP)和双极等离子体电切术(PKRP)治疗高龄高危良性前列腺增生(BPH)的有效性及安全性。方法采用80W KTP PVP术治疗高龄高危患者共166例,其中62例行PKRP+PVP术。患者年龄80~95岁,平均83岁;前列腺体积17~278ml,平均74ml,其中80ml以上36例(21.7%)。患者平均国际前列腺症状评分(IPSS)为(28±4)分,平均生活质量评分(QOL)为(5.3±0.7)分;入院时留置导尿者72例(43.4%)。其余患者平均最大尿流率(Qmax)为(6.9±3.2)ml/s。结果本组平均手术时间(92±28)min,平均汽化能量(127±99)kJ;平均术中出血量(121±92)ml,其中9例术中有较明显出血并需要输血,均为术前服用抗凝药物者。术中未出现电切综合征(TURS)迹象。术后1~4d后拔除导尿管,平均(2.9±1.1)d,其中9例因排尿困难、2例因残尿过多而再次留置导尿1~3d拔管排尿通畅,其余患者术后均排尿通畅。出院时IPSS平均(11±2)分,QOL平均(2.0±0.6,Qmax平均(12.6±5.2)ml/s;术后3月和6月的IPSS、QOL分别为(11±4)分,(11±3)分和(1.9±0.4)分,(1.8±0.4)分。结论PVP术或PKRP+PVP术是治疗高龄高危前列腺增生安全而有效的方法,可明显提高患者生活质量。     

Compatibility of porcine and human interleukin 2: implications for xenotransplantation

BACKGROUND: Xenotransplantation provides a possible solution to the severe shortage of allogeneic organ donors. The pig, which shares many physiological similarities with humans, makes it an optimal species for preclinical experimentation and clinical applications. Interleukin 2 (IL2) is a potent growth factor secreted primarily by T helper lymphocytes and it is vital to the cellular expansion required for a productive immune response and the development and peripheral expansion of CD4+CD25+ regulatory T cells. Therefore, it is essential to understand of the compatibility of IL2 between pigs and humans. METHODS: We first compared the cDNA and protein sequences and the crystal structures of human and porcine IL2 and IL2 receptors, respectively. The effect of IL2 to induce T cell proliferation was determined by 3H-thymidine incorporation and cell cycle detection. RESULTS: Porcine IL2 induced very limited proliferation of human lymphocytes while it functioned well on porcine lymphocytes. Human IL2 had remarkably reduced effects on porcine lymphocytes whereas it worked well on human lymphocytes. CONCLUSION: Our present study showed that the interaction of IL2 and IL2R across species might have defects. Together with the wide physiological functions of IL2, our data indicated that physiological disorders could be caused by the poor function of xenogeneic donor IL2 on host cells in full hematopoietic chimera. Our data suggested an additional potential advantage for the mixed xenogeneic chimeras.     

Confirmation and prevention of intestinal barrier dysfunction and bacterial translocation caused by methotrexate

When intestinal barrier function is damaged bacterial translocation (BT) can occur. The injury to intestinal barrier function caused by chemotherapy has been investigated in some studies, however, definitive evidence of BT caused by chemotherapy is lacking. The aim of this study was to investigate small intestinal barrier dysfunction and BT and to evaluate the preventive effect of granulocyte colony-stimulating factor (G-CSF) on intestinal barrier dysfunction and BT in a rat model of chemotherapy. Sprague-Dawley rats were treated with methotrexate (MTX; 3.5 mg/kg) for 3 days to induce intestinal barrier dysfunction and BT, gavaged Escherichia coli TG1 labeled with green fluorescent protein for 2 days to track BT, and G-CSF (10 μg/kg) for 4 days to prevent intestinal barrier dysfunction and BT. Intestinal permeability was measured by the urinary excretion rate of lactulose and mannitol following administration by gavage. Representative tissue specimens from the mesenteric lymph nodes, spleen, liver, and kidney were aseptically harvested for bacteria culture in ampicillin-supplemented medium. Light microscopy was performed on intestinal samples. MTX induced significant mucosal and villous atrophy in ileum and significantly increased intestinal permeability. MTX also induced noticeable BT. G-CSF significantly increased the mucosal thickness and villous height of the ileum and decreased intestinal permeability and BT. In conclusion, MTX caused intestinal barrier dysfunction and BT, and G-CSF prevented intestinal barrier dysfunction and BT.     

慢性B淋巴细胞白血病的免疫表型及预后的临床研究

目的探讨慢性B淋巴细胞白血病(B-CLL)的免疫表型特征及预后。方法采用CD45/SSC双参数散点图设门,应用流式细胞术对37例初诊CLL患者骨髓标本进行免疫分型,另外选择同期32例急性淋巴细胞白血病(ALL)患者作为对照。结果①37例患者中HLA-DR、CD19、CD20及CD5表达最常见,阳性率依次为100.0%、100.0%、95.0%及86.4%,CLL患者无一例表达CD34;②与ALL mature-B相比,CLL高表达CD5(P<0.05),而CD10和CD34的表达率则偏低(P<0.05),HLA-DR、CD19及CD20的表达差异无统计学意义(P>0.05)。结论 CLL的免疫表型特征为CD5、CD19及CD23的共表达;CD19和CD20诊断CLL的灵敏度高,但特异性不高;CD5,CD10可作为B-CLL与Mature-B-ALL鉴别诊断的特异性指标。