舌下脱敏治疗儿童间歇性变应性鼻炎、支气管哮喘50例疗效分析

目的：评估舌下含服粉尘螨滴剂治疗尘螨致敏的间歇性过敏性哮喘、变应性鼻炎患儿的临床疗效;观察单一脱敏对单一粉尘螨过敏和双螨过敏的疗效。方法：收集2012年6月至2013年6月到我院儿科确诊的螨过敏间歇性变应性鼻炎（AR）和过敏性哮喘（AS）患儿50例,按皮肤点刺试验（SPT）结果将患儿分为粉尘螨组27例和双螨组23例。记录所有患儿治疗前和治疗12个月后的症状评分、用药情况评分和视觉模拟评分（VAS）。用酶联免疫吸附（ELISA）法检测部分患儿的血清特异性免疫球蛋白G亚型4（sIgG4）水平。结果：①舌下脱敏治疗12个月后,患儿的症状评分、用药评分和VAS评分均有显著性降低,和舌下脱敏治疗前比较差异有统计学意义（P<0.01）;患儿的血清sIgG4水平脱敏治疗前为（79.21±78.13）,脱敏治疗12个月后为（137.95±92.01）,治疗前后比较差异具有统计学意义（P<0.01）。②单一粉尘螨舌下脱敏治疗单一粉尘螨和双螨过敏患者12个月后,患儿的症状评分、用药评分和VAS评分均显著性降低,且和治疗前相比差异具有统计学意义（P<0.01）;治疗12个月后,粉尘螨组和双螨组的症状评分、用药评分和VAS评分比较差异无统计学意义（P>0.05）。结论：舌下脱敏治疗螨过敏引起的间歇性哮喘鼻炎患儿具有显著效果,单一粉尘螨脱敏治疗单一粉尘螨过敏和双螨过敏患儿的疗效相当。     

经伤椎单双侧置钉与不置钉治疗胸腰段骨折的疗效比较

目的比较后路短节段经伤椎单双侧置钉与不置钉治疗胸腰段椎体骨折的临床疗效。方法采用回顾性分析的方法,自 2017年 1—12月,安徽医科大学附属六安医院采用后路短节段椎弓根钉内固定术治疗胸腰段单一椎体骨折病人 98例。根据伤椎置钉多少分为三组:跨伤椎置钉组( A组) 32例,单侧伤椎置钉组( B组) 28例,双侧伤椎置钉组( C组) 38例,随访时间范围为 12~20个月,平均 16.2个月,对三组病人的术中、术后及末次随访相关指标进行比较。结果三组病人的手术时间［A组(88.00±11.75)min,B组( 87.63±11.85)min,C组( 89.97±11.28)min,F＝0.405］、术中出血量［A组( 107.81±16.09)mL,B组(111.72±20.02)mL,C组( 113.43±19.18)mL,F＝0.825］、术后疼痛视觉模拟评分( VAS)［A组( 3.19±0.93)分, B组( 3.36±0.83)分, C组(3.34±0.77)分, F＝0.383］、术后第 3天的伤椎前缘高度比［A组(96.07±2.23)%,B组(96.79±2.90)%,C组( 96.75±3.76)%,F＝0.545］及矢状位 Cobb角［A组( 5.07±1.95)°,B组( 4.40±1.34)°,C组( 4.29±1.66)°,F＝2.060］比较均差异无统计学意义( P＞0.05)。末次随访时, B、C组病人的伤椎前缘高度比［A组( 92.79±1.97)%,B组( 94.12±2.00)%,C组( 95.00±1.70)%,F＝12.079］、矢状位 Cobb角［A组( 7.77±1.87)°,B组( 6.47±1.54)°,C组( 6.38±1.58)°,F＝7.046］、伤椎复位丢失情况［高度丢失: A组( 3.01±1.18)%,B组( 2.26±0.87)%,C组( 1.83±0.60)%,F＝15.175;角度丢失: A组(2.68±1.06)°,B组( 2.07±0.85)°,C组(2.11±0.86)°,F＝4.349］与A组病人相比差异有统计学意义( P＜0.05),B组与 C组病人相比差异无统计学意义( P＞0.05)。结论经伤椎置钉与传统的跨伤椎置钉相比在治疗胸腰段椎体骨折上能更有效的降低后期复位椎体高度的丢失和减少迟发型后凸畸形的发生;单侧置钉与双侧置钉疗效相似。     

Upregulation of drug sensitivity of multidrug-resistant SGC79 01/VCR human gastric cancer cells by bax gene transduction

Objective To investigate the role of bax in a vincristine （VCR）-induced multidrug-resi stant （MDR） human gastric cancer cell line, SGC7901/VCR, in which the Bax prot ein expression level was significantly lower compared with that in parent cells .Methods A bax eukaryotic expression vector was constructed and transfected into SGC7901/ VCR cells by lipofectamine, and resistant clones were selected by G418. Western blotting detected Bax expression in transfectants. Tetrazolium blue (MTT) assa y evaluated the differences in drug sensitivity and cell cycle changes of transf ectants were analyzed using flowcytometry (FCM). Results The bax eukaryotic expression vector was constructed and transfected into SGC790 1/VCR cells. Through G418 selection, resistant clones were obtained. Western b lotting demonstrated that the expression of Bax protein was markedly increased i n bax transduced cells. These cells were more sensitive to adriamycin (ADR) and VCR than mock vector transducted cells. Moreover, bax transfection enhanced AD R-induced apoptosis and VCR-induced G(2)/M phase arrest of SGC7901/VCR cells. Conclusion Bax was involved in the MDR of SGC7901/VCR cells.     

The relationship between tumor necrosis factor-a gene polymorphisms and acute severe pancreatitis

Objective To investigate the relationship between the presence of the TNF2 allele and plasma concentrations of tumor necrosis factor-α (TNFα) and soluble TNF receptor (sTNF-R) with the development of acute severe pancreatitis (ASP) and severe sepsis.Methods Genomic DNA was prepared from peripheral blood leukocytes. The TNF1 and TNF2 biallelic polymorphisms were identified by analyzing Ncol-digested DNA fragments obtained from PCR products. Plasma levels of TNFa and sTNF-R were measured by EASIA.Results The overall TNF2 allele frequency in ASP patients was comparable to that found in healthy volunteers (29. 2% vs. 29. 3% , P>0. 05). Severe sepsis occurred in 26 of 72 patients. Patients with severe sepsis showed a significantly higher prevalence of TNF2 than those without (46. 2% vs. 19.6%, P<0. 05). Plasma TNFα, sTNF-R Ⅰ, and sTNF-R Ⅱ levels were (36± 31) pg/ml, (5. 4± 3.5) ng/ml, and (11.2±7.8) ng/ml, respectively, in patients with severe sepsis, and (31 ±25) pg/ml, (4.6±3.8) ng/ml, and (8.8      

CT引导下弹簧圈定位在肺小结节（直径≤15 mm）单操作孔胸腔镜手术中应用的回顾性队列研究

目的探讨CT引导下弹簧圈定位在肺小结节(直径≤15 mm)手术中的应用价值。方法回顾性分析2018年8月至2019年12月在南京鼓楼医院行单操作孔胸腔镜手术治疗175例肺小结节患者的临床资料。根据术前是否行CT引导下弹簧圈定位将患者分为定位组[84例,其中男34例、女50例,年龄(57.8±8.8)岁]和非定位组[91例,其中男46例、女45例,年龄(57.6±10.8)岁]。对定位成功率、定位时间、并发症发生率、手术及术后情况等相关指标进行统计分析。结果定位组84例患者均定位成功,定位时间为(19.0±3.6)min。发生少量气胸19例(22.6%)、肺内出血4例(4.8%)、弹簧圈移位2例(2.4%);轻度疼痛6例(7.1%)、中度疼痛3例(3.6%)、重度疼痛1例(1.2%)。两组性别(P=0.181)、年龄(P=0.673)、结节位置(P=0.167)、病变性质(P=0.244)、胸腔镜手术中转开胸率(P=0.414)、结节一次性切除率(P=0.251)、术后住院时间(P=0.207)方面差异无统计学意义;结节大小(P<0.001)、结节性质(P<0.001)、结节...     

乳腺癌超声弹性成像面积比与其组织学分级、临床分期关系的初步研究

目的探讨乳腺超声弹性成像面积比（AR）对乳腺癌患者病情发展及预后评估的参考价值。方法回顾性分析105例经病理结果证实的乳腺癌患者临床资料,术前行乳腺常规超声及超声弹性成像检查,分析乳腺癌患者不同组织学分级间、不同临床分期间AR参数的变化情况。结果术前以AR≥1.5为诊断恶性的标准,73例恶性,诊断符合率为69.52%。超声弹性成像AR在各组织学分级、临床分期组间差异有统计学意义（P〈0.05）：组织学分级Ⅰ～Ⅲ级的AR值分别为1.210±0.018、1.470±0.126、1.600±0.028,临床分期Ⅰ～Ⅳ期AR值分别为1.230±0.056、1.540±0.017、1.580±0.025及1.620±0.023,即AR在组织分级较高及临床分期较晚时增大。结论 AR可为乳腺癌病情发展及预后评估提供有价值的参考信息。     

传统开腹胆囊切除术与腹腔镜下胆囊切除术的临床疗效对比

目的分析对比传统开腹胆囊切除术与腹腔镜下胆囊切除术的临床疗效。方法从我院胆囊患者当中选取180例为研究对象,并把其随机的分为对照组和观察组,每组90例,对照组患者进行开腹胆囊切除术,观察组患者进行腹腔镜下胆囊切除术。结果观察组患者的手术时间、平均住院时间以及术后不良症状发生率都要明显的低于对照组,两组之间存在明显的差异,具有统计学意义(P<0.05)。结论相对于传统的开腹胆囊切除术,腹腔镜下胆囊切除术具有手术时间短、康复快、并发症少等优点,因此具有更好的临床治疗效果。     

Y-configuration double-stent-retriever thrombectomy for refractory thrombus in middle cerebral artery bifurcation: A case report

Rationale:Stent retriever mechanical thrombectomy is a recommended treatment for acute ischemic stroke. However, refractory thrombus in artery bifurcation can reduce the rate of successful revascularization.Patient concerns:A 72-year-old male, owing to the acute onset of almost complete right-sided hemiplegia and global aphasia, received bridging therapy. National Institutes of Health Stroke Scale score was 16 at the time of admission.Diagnoses:Cerebral digital subtraction angiography revealed occlusion of the M1 segment of the left MCA.Interventions:Thrombectomy with 3 passes of the Solitaire FR device (Medtronic, Minneapolis, MN) was unsuccessful. Two stent retrievers were inserted in parallel by one microcatheter access point to each M2 branch, and then both stents were gradually retrieved out of the catheter while continuous suction was maintained.Outcomes:After thrombectomy, subsequent follow-up angiograms showed mTICI 3 reperfusion of MCA. The patient has mRS 2 at discharge and the 3-month mRS score after stroke is 1 score.Lessons:The presented Y-configuration double-stent-retriever thrombectomy technique constitutes a safe and effective rescue treatment method for refractory thrombus in MCA bifurcation.     

Prevalence and associated factors of periodontal conditions among 55- to 74-year-old adults in China: results from the 4th National Oral Health Survey

Clinical Oral Investigations - The purpose of this study was to assess periodontal health conditions among 55- to 74-year-old Chinese people and to analyze the factors associated with periodontal...     

干细胞移植治疗脊髓损伤后排尿障碍的可行性

一直以来,脊髓损伤因其高致残率而被视为医学界的一大难题。在脊髓损伤的诸多并发症中,泌尿系统并发症是脊髓损伤患者最主要的死因之一。由于损伤的神经细胞无法再生,目前对于脊髓损伤患者排尿障碍的各种治疗效果都十分有限。然而,随着干细胞研究的不断进展,越来越多的实验表明干细胞移植治疗可以改善脊髓损伤后的排尿功能,提示该方法具有一定的可行性。本文就近年来关于干细胞移植治疗脊髓损伤后排尿障碍的研究进展,从常用干细胞种类、干细胞移植治疗的机制、干细胞移植时间、干细胞移植途径几个方面做一综述,并提出通过干细胞体外分化得到能够分泌5-羟色胺的神经细胞来进行移植治疗脊髓损伤后排尿障碍的新思路。