全文获取类型
收费全文 | 708篇 |
免费 | 32篇 |
国内免费 | 37篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 28篇 |
妇产科学 | 12篇 |
基础医学 | 70篇 |
口腔科学 | 32篇 |
临床医学 | 64篇 |
内科学 | 158篇 |
皮肤病学 | 86篇 |
神经病学 | 22篇 |
特种医学 | 85篇 |
外科学 | 65篇 |
综合类 | 20篇 |
预防医学 | 40篇 |
眼科学 | 5篇 |
药学 | 59篇 |
中国医学 | 1篇 |
肿瘤学 | 29篇 |
出版年
2021年 | 5篇 |
2018年 | 8篇 |
2016年 | 7篇 |
2014年 | 13篇 |
2013年 | 14篇 |
2012年 | 25篇 |
2011年 | 30篇 |
2010年 | 23篇 |
2009年 | 18篇 |
2008年 | 24篇 |
2007年 | 46篇 |
2006年 | 24篇 |
2005年 | 30篇 |
2004年 | 17篇 |
2003年 | 17篇 |
2002年 | 15篇 |
2001年 | 18篇 |
2000年 | 10篇 |
1999年 | 14篇 |
1998年 | 19篇 |
1997年 | 15篇 |
1996年 | 18篇 |
1995年 | 9篇 |
1994年 | 15篇 |
1993年 | 22篇 |
1992年 | 9篇 |
1991年 | 11篇 |
1990年 | 11篇 |
1989年 | 19篇 |
1988年 | 18篇 |
1987年 | 19篇 |
1986年 | 16篇 |
1985年 | 19篇 |
1984年 | 16篇 |
1983年 | 8篇 |
1981年 | 11篇 |
1980年 | 14篇 |
1979年 | 9篇 |
1978年 | 5篇 |
1977年 | 5篇 |
1975年 | 5篇 |
1974年 | 9篇 |
1973年 | 17篇 |
1972年 | 18篇 |
1971年 | 12篇 |
1970年 | 9篇 |
1969年 | 6篇 |
1968年 | 10篇 |
1967年 | 8篇 |
1966年 | 4篇 |
排序方式: 共有777条查询结果,搜索用时 0 毫秒
51.
52.
53.
54.
55.
Levy JI Welker-Hood LK Clougherty JE Dodson RE Steinbach S Hynes HP 《Environmental health : a global access science source》2004,3(1):13
Background
Children in urban public housing are at high risk for asthma, given elevated environmental and social exposures and suboptimal medical care. For a multifactorial disease like asthma, design of intervention studies can be influenced by the relative prevalence of key risk factors. To better understand risk factors for asthma morbidity in the context of an environmental intervention study, we conducted a detailed baseline evaluation of 78 children (aged 4–17 years) from three public housing developments in Boston. 相似文献56.
Hernroth B Baden SP Holm K André T Söderhäll I 《Aquatic toxicology (Amsterdam, Netherlands)》2004,70(3):223-231
Manganese (Mn) is one of the most abundant elements on earth, particularly in the soft bottom sediments of the oceans. As a micronutrient Mn is essential in the metabolic processes of organisms. However, at high concentrations the metal becomes a neurotoxin with well-documented effects. As a consequence of euthrophication, manganese is released from bottom sediments of coastal areas and the Norway lobsters, Nephrops norvegicus, can experience high levels of bioavailable Mn(2+). Here, we present the first report showing that Mn also affects several fundamental processes in the mobilisation and activation of immunoactive haemocytes. When N. norvegicus was exposed to a realistic [Mn(2+)] of 20 mg l(-1) for 10 days 24.1 microg ml(-1) was recorded in the haemolymph. At this concentration the total haemocyte count was reduced by ca. 60%. By using BrdU as a tracer for cell division, it was shown that the proliferation rate in the haematopoietic tissue did not increase, despite the haemocytepenia. A gene coding for a Runt-domain protein, known to be involved in maturation of immune active haemocytes in a variety of organisms, was identified also in haemocytes of N. norvegicus. The expression of this gene was >40% lower in the Mn-exposed lobsters as judged by using a cDNA probe and the in situ hybridisation technique. In response to non-self molecules, like lipopolysaccharide (LPS), the granular haemocytes of arthropods are known to degranulate and thereby release and activate the prophenoloxidase system, necessary for their immune defence. A degranulation assay, tested on isolated granular haemocytes, showed about 75% lower activity in the Mn-exposed lobsters than that for the unexposed. Furthermore, using an enzymatic assay, the activation per se of prophenoloxidase by LPS was found blocked in the Mn-exposed lobsters. Taken together, these results show that Mn exposure suppressed fundamental immune mechanisms of Norway lobsters. This identifies a potential harm that also exists for other organisms and should be considered when increasing the distribution of bioavailable Mn, as has been done through recently introduced applications of the metal. 相似文献
57.
58.
L. Joseph Wheat M. Hong Nguyen Barbara D. Alexander David Denning Angela M. Caliendo G. Marshall Lyon Lindsey R. Baden Francisco M. Marty Cornelius Clancy Emily Kirsch Pamela Noth John Witt Michele Sugrue John R. Wingard 《Journal of clinical microbiology》2014,52(6):2108-2111
Research to develop and validate novel methods for diagnosis of aspergillosis based on detection of galactomannan requires the use of clinical specimens that have been stored frozen. Data indicating that galactomannan remains stable when frozen are scant. The objective of this study was to determine the stability of galactomannan in clinical specimens stored at −20°C that were positive in the Platelia Aspergillus enzyme immunoassay when initially tested. Prospective real-time testing of serum and bronchoalveolar lavage (BAL) fluid pools from positive and negative patient specimens showed no decline in galactomannan index (GMI) over 11 months at −20°C and no development of positive reactions in the negative-control pool. Retrospective testing of positive specimens that had been stored at −20°C for 5 years showed that 28 of 30 serum (n = 15) or BAL (n = 15) specimens remained positive. These findings support the use of frozen serum or BAL specimens stored for at least 5 years in evaluation of diagnostic tests based on detection of galactomannan. 相似文献
59.
Adverse reaction to intravenous gadoteridol 总被引:1,自引:0,他引:1
60.
Benson J Hahn F March T McDonald J Sopori M Seagrave J Gomez A Bourdelais A Naar J Zaias J Bossart G Baden D 《Journal of toxicology and environmental health. Part A》2004,67(18):1443-1456
Brevetoxins are potent neurotoxins produced by the marine dinoflagellate Karenia brevis. Exposure to brevetoxins may occur during a K. brevis red tide when the compounds become aerosolized by wind and surf. This study assesses possible adverse health effects associated with short-term inhalation exposure to brevetoxin 3. Male F344/Crl/Br rats were exposed to 500 microg brevetoxin 3/m3 by nose-only inhalation for 0.5 or 2 h/d for 5 consecutive days. Control rats were sham exposed for 2 h to vehicle. Calculated deposited brevetoxin doses were 8.3 and 33 microg/kg/d for the low- and high-dose groups, respectively. At the termination of exposures, only body weights of the high-dose group (Group B) were significantly below control values. By immunohistochemistry (IHC), small numbers of splenic and peribronchiolar lymphoid tissue macrophages stained positive for brevetoxin, while nasal mucosa, liver, and brain were IHC negative for brevetoxin. No gross or microscopic lesions were observed in any tissue examined. There was no biochemical evidence of cytotoxicity or inflammation in bronchoalveolar lavage fluid. Alveolar macrophages showed some evidence of activation following brevetoxin exposure. Humoral-mediated immunity was suppressed in brevetoxin-exposed rats as indicated by a >70% reduction in splenic plaque-forming cells in brevetoxin-exposed animals compared to controls. Results suggest that the immune system may be a target of toxicity following brevetoxin inhalation. Future studies will focus on identification of a no-effect level and mechanisms underlying brevetoxin-induced immune suppression. 相似文献