全文获取类型
收费全文 | 4178篇 |
免费 | 191篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 72篇 |
儿科学 | 78篇 |
妇产科学 | 45篇 |
基础医学 | 477篇 |
口腔科学 | 55篇 |
临床医学 | 268篇 |
内科学 | 1104篇 |
皮肤病学 | 67篇 |
神经病学 | 275篇 |
特种医学 | 161篇 |
外科学 | 731篇 |
综合类 | 20篇 |
预防医学 | 179篇 |
眼科学 | 93篇 |
药学 | 292篇 |
中国医学 | 7篇 |
肿瘤学 | 462篇 |
出版年
2023年 | 20篇 |
2022年 | 72篇 |
2021年 | 111篇 |
2020年 | 32篇 |
2019年 | 61篇 |
2018年 | 72篇 |
2017年 | 67篇 |
2016年 | 69篇 |
2015年 | 70篇 |
2014年 | 113篇 |
2013年 | 133篇 |
2012年 | 229篇 |
2011年 | 226篇 |
2010年 | 130篇 |
2009年 | 106篇 |
2008年 | 188篇 |
2007年 | 216篇 |
2006年 | 166篇 |
2005年 | 195篇 |
2004年 | 145篇 |
2003年 | 160篇 |
2002年 | 181篇 |
2001年 | 172篇 |
2000年 | 176篇 |
1999年 | 133篇 |
1998年 | 35篇 |
1997年 | 46篇 |
1996年 | 33篇 |
1995年 | 22篇 |
1994年 | 21篇 |
1993年 | 21篇 |
1992年 | 102篇 |
1991年 | 81篇 |
1990年 | 83篇 |
1989年 | 93篇 |
1988年 | 92篇 |
1987年 | 65篇 |
1986年 | 68篇 |
1985年 | 73篇 |
1984年 | 50篇 |
1983年 | 45篇 |
1982年 | 16篇 |
1981年 | 13篇 |
1979年 | 30篇 |
1978年 | 10篇 |
1977年 | 11篇 |
1976年 | 10篇 |
1975年 | 19篇 |
1973年 | 13篇 |
1968年 | 13篇 |
排序方式: 共有4386条查询结果,搜索用时 15 毫秒
121.
122.
Genetic Polymorphism of Plasminogen: A New Basic Variant (PLG B) and Population Study in Japanese 总被引:3,自引:0,他引:3
Dr. Hiroaki Nishimukai Yoshio Kera Kiyoshi Sakata and Kichihei Yamasawa 《Vox sanguinis》1981,40(6):422-425
Abstract. Genetic polymorphism of human plasminogen in the Japanese population was studied using agarose gel isoelectric focusing followed by immunofixation. A new basic variant, PLG B, was found as a heterozygous state PLG l-B, which was genetically determined. The allele frequencies calculated from 258 individuals were PLG1 =0.958, PLG2 =0.020 and PLGB =0.022. 相似文献
123.
Pancreatic cancer represents a major challenge to oncologists because of its high chemoresistant nature and dismal outcomes. Conventional therapy for advanced disease relied for a long time on palliative 5‐fluorouracil (5‐FU)‐based chemotherapy, but with unsatisfactory results. The introduction of the novel antimetabolite gemcitabine provides new optimism for patients with advanced pancreatic cancer, as multiple clinical trials have demonstrated the superiority of gemcitabine over 5‐FU and other agents for these patients. The benefits of gemcitabine over conventional therapies include improved response rate and enhanced survival, as well as improvement in disease‐related symptoms and quality of life in these patients. With these data, gemcitabine is widely accepted worldwide as the therapy of choice by many oncologists for advanced pancreatic cancer. The current review presents an overview of the clinical studies of gemcitabine over the past decade for the treatment of patients with advanced pancreatic cancer. Other investigational regimens or uses (e.g., fixed dose‐rate infusion, intraarterial infusion, adjuvant use, chemo‐radiation, etc) are also reviewed. 相似文献
124.
Sakata Y Iwakiri R Amemori S Yamaguchi K Fujise T Otani H Shimoda R Tsunada S Sakata H Ikeda Y Ando T Nakafusa Y Fujimoto K 《European journal of gastroenterology & hepatology》2008,20(7):629-633
BACKGROUND: Ulcerative colitis (UC) is a chronic inflammatory bowel disease associated with recurring inflammation of the colorectal mucosa. Recently, cytapheresis has emerged as a new treatment for patients with UC. Removal methods are mainly performed with beads [granulocyte and monocyte/macrophage adsorptive apheresis (GMCAP)] or filters [leukocytapheresis (LCAP)]. Both treatments have been reported to be effective for active UC. There have been few trials, however, comparing the efficacy of GMCAP and LCAP. In this study, we prospectively evaluated the efficacy of LCAP and GMCAP for the treatment of active UC. METHODS: Thirty-nine patients [18 male, 21 female; mean age 38.7 years; duration of disease 6 years; clinical activity index (CAI) >6 points] with moderate-to-severe active UC were randomly assigned to the LCAP (n=21) or GMCAP group (n=17). Adacolumn (cellulose acetate beads; Japan Immunoresearch Laboratories, Takasaki, Japan) for GMCAP and Cellsorba EX (polyethylene phthalate fibers; Asahi Medical Co. Ltd, Tokyo, Japan) for LCAP were used for leukocyte removal. Patients received two sessions of cytapheresis in the first week, followed by four weekly administrations. Steroid doses were tapered if patients achieved clinical improvement. When the CAI score had decreased by 5 points or more, the patient was considered to have improved. RESULTS: Thirteen patients in the GMCAP group and 14 in the LCAP group achieved clinical improvement. No significant difference was found in clinical response and clinical course between LCAP and GMCAP. Hemoglobin levels were significantly decreased immediately after one session of cytapheresis in the LCAP group. No severe adverse effects were observed in any of the patients. No significant differences were observed in any clinical parameters predictive of a response to either LCAP or GMCAP. But in all patients receiving cytapheresis, a high CAI score was a significant risk factor for treatment failure. All of the cytapheresis nonresponders had CAI scores >or=16. CONCLUSION: Both GMCAP and LCAP were effective treatments for active UC. Patients with severe UC and a high CAI score were, however, refractory to treatment. 相似文献
125.
Yamamoto K Masuyama T Sakata Y Doi R Ono K Mano T Kondo H Kuzuya T Miwa T Hori M 《Cardiovascular research》2000,46(3):421-432
OBJECTIVE: Although isolated diastolic heart failure with preserved left ventricular (LV) systolic function frequently occurs, regulation of local neurohumoral factors in the transition from diastolic dysfunction without signs of heart failure to diastolic failure, a target for therapeutic strategy, remains to be clarified, partly because of a lack of animal models. Our laboratory recently demonstrated that Dahl-Iwai salt-sensitive (Dahl-S) rats fed on a high-salt diet since 7 weeks of age develop hypertension followed by compensated LV hypertrophy at 13 weeks and transition to isolated diastolic heart failure at 19 weeks. METHODS: Gene expression of the components of the renin-angiotensin system, endothelin (ET) system and natriuretic peptide system in the left ventricle was investigated in the transition to isolated diastolic heart failure in this model. RESULTS: The compensated ventricular hypertrophy was associated with slight increases in angiotensin-converting enzyme (ACE) and angiotensin II type-1a (AT1a) receptor mRNA levels. Although preproET-1 (ppET-1) and ET-converting enzyme-1 (ECE-1) mRNA levels were not increased, mRNA levels of ET type-A (ETA) and ET type-B (ETB) receptors were increased. Atrial natriuretic peptide (ANP) mRNA level increased, but not brain natriuretic peptide (BNP) mRNA level. At the decompensated failing stage (at 19 weeks), ACE mRNA level further increased without downregulation of ATla receptor mRNA level. The mRNA levels of ppET-1 and ECE-1 increased with persistent upregulation of mRNA levels of ETA and ETB receptors, and immunohistochemical staining for ET-1 was found at endothelial cells and myocytes. BNP mRNA level increased with a further increase in ANP mRNA level. CONCLUSIONS: The transition to isolated diastolic heart failure in hypertrophied hearts was associated with preserved gene expression of the renin-angiotensin system and 'overdrive' of gene expression of the ET system. BNP gene expression is likely to be activated by the progression of diastolic failure rather than by LV hypertrophy alone. 相似文献
126.
127.
Noriko Komatsu Stephanie Win Minglu Yan Nam Cong-Nhat Huynh Shinichiro Sawa Masayuki Tsukasaki Asuka Terashima Warunee Pluemsakunthai George Kollias Tomoki Nakashima Hiroshi Takayanagi 《The Journal of clinical investigation》2021,131(6)
In rheumatoid arthritis (RA), osteoclastic bone resorption causes structural joint damage as well as periarticular and systemic bone loss. Periarticular bone loss is one of the earliest indices of RA, often preceding the onset of clinical symptoms via largely unknown mechanisms. Excessive osteoclastogenesis induced by receptor activator of NF-κB ligand (RANKL) expressed by synovial fibroblasts causes joint erosion, whereas the role of RANKL expressed by lymphocytes in various types of bone damage has yet to be elucidated. In the bone marrow of arthritic mice, we found an increase in the number of RANKL-expressing plasma cells, which displayed an ability to induce osteoclastogenesis in vitro. Genetic ablation of RANKL in B-lineage cells resulted in amelioration of periarticular bone loss, but not of articular erosion or systemic bone loss, in autoimmune arthritis. We also show conclusive evidence for the critical contribution of synovial fibroblast RANKL to joint erosion in collagen-induced arthritis on the arthritogenic DBA/1J background. This study highlights the importance of plasma-cell RANKL in periarticular bone loss in arthritis and provides mechanistic insight into the early manifestation of bone lesion induced by autoimmunity. 相似文献
128.
Masahiro Kanatani Tadashi Adachi Ryoichi Sakata Yoko Nishimura Yoshiaki Saito Yoshihiro Maegaki Yasuhiro Watanabe Ritsuko Hanajima 《Brain & development》2021,43(3):486-489
IntroductionDravet syndrome (DS) is severe myoclonic epilepsy in infancy and associated with a heterozygous mutation of the gene for the sodium channel alpha 1 subunit (SCN1A). Recently, adult patients with DS have been reported to show parkinsonism, but no corresponding neuroimaging data are available. Here, we present neuroimaging data in 2 adult patients with DS showing parkinsonian symptoms.Case reportCase 1: A man who had intractable seizures from the age of 1 year and 2 months was diagnosed with DS at 7 with a mutation in the SCN1A gene. At 18, he had parkinsonian symptoms such as masked face and bradykinesia. At 20, he was admitted to our department. Dopamine transporter single-photon emission computed tomography (DAT SPECT) showed no decrease in striatal binding of 123I–N–ω–fluoropropyl–2β–carbomethoxy–3β–(4–iodophenyl) nortropane (123I-FP-CIT), and myocardial scintigraphy showed no decrease in cardiac uptake of 123I-metaiodobenzylguanidine (123I-MIBG). Levodopa showed no significant improvement in his symptoms. Case 2: A woman who had febrile seizures at 4 months of age and myoclonic seizures at 1 year and 5 months was diagnosed with DS at 31. She had myoclonus, resting tremor, hypertonia, antecollis, crouch gait, and bradykinesia. DAT SPECT imaging showed no decrease in striatal FP-CIT binding, and levodopa did not improve her symptoms.DiscussionThe normal DAT SPECT and 123I-MIBG results suggest that dopaminergic neurons projecting onto striatal neurons were not impaired in our patients, explaining the lack of response to levodopa. Thus, dopamine imaging can help to guide treatment decisions in patients with DS and parkinsonism. 相似文献
129.
Daisuke Yoshioka Koichi Toda Taichi Sakaguchi Shigeru Miyagawa Hiroyuki Nishi Yasushi Yoshikawa Satsuki Fukushima Shunsuke Saito Tetsuya Saito Ikuko Shibasaki Yasushi Sakata Tomohito Ohtani Yoshiki Sawa 《Journal of artificial organs》2013,16(3):386-388
Continuous-flow left ventricular assist devices (LVADs) provide acceptable clinical results, but the long waiting period for heart transplantation leads to diverse complications. LVAD support can cause reverse left ventricular (LV) remodeling that results in the improvement of LV function and allows LVAD removal. We present a case of successful removal of a DuraHeart LVAD because of sufficient recovery of LV function. Before LVAD removal, we conducted an “LVAD weaning test” by decreasing pump speed and performing an additional normal saline infusion test. We consider that the LVAD weaning test can be used in place of the “pulsatile LVAD off test.” 相似文献