重组Sj31-b的表达及其免疫诊断价值的研究

目的 探讨日本血吸虫重组31KD蛋白片（rSJ31-b)的免疫诊断价值。方法 表达并纯化rSj31-b，用免疫印迹方法（EITB）检测纯化的rSj-b的免疫活性，以rSj31-b-ELISA分别检测慢性日本血吸虫病人及正常人的血清。结果 纯化的重组Sj31-b具有较好的免疫活性；用其rSj31-b－ELISA检测52份慢性日本血吸虫病人血清及83份正常人血清，敏感性和特异性与常规应用的天然抗原SEA（可溶性虫卵抗原）差异无显性。检测治疗12个月后的慢性日本血吸虫病人血清，阴转率85.0%，明显优于SEA－ELISA。结论 rSj31-b具有较好的敏感性和特异性，且有一定的疗效考核价值，可替代SEA用于慢性日本血吸虫病人的诊断。     

尼可地尔预先给药对心肌缺血再灌注兔心肌细胞凋亡的影响

目的观察尼可地尔预先给药对心肌缺血再灌注兔心肌细胞凋亡的影响。方法 雄 性新西兰白兔40只,随机分为5组(n=8),假手术组(Sham组):仅穿线,不结扎;IR组:缺血30 min再 灌注120min;Nic组:缺血前10min静脉注射尼可地尔100μg·kg-1负荷量,随后以10μg·kg-1·min-1的 速率持续静脉输注至缺血前即刻;Nic 5-HD、Nic Gli组:在缺血前20 min静脉注射五羧基葵酸钠或 格列本脲5 mg·kg-1,其余的处理同Nic组。再灌注120 min时处死兔,计算心肌梗塞面积,通过两种染 色方法检测心肌细胞凋亡情况,观察心肌超微结构改变,并用免疫组织化学方法测定活化的caspase-3 蛋白表达。结果与IR组比较,Nic组心肌梗塞面积减小,早期凋亡细胞数减少,活化的caspase-3蛋 白表达下降(P<0．05),与Sham组比较,Nic组心肌仍有一定损伤,但较IR组明显改善。与Nic组比 较,Nic 5-HD、Nic Gli组心肌梗塞面积、早期凋亡细胞数、活化的caspase-3蛋白表达增加(P< 0．05)。结论尼可地尔预先给药对缺心肌血再灌注损伤有一定的保护作用,主要通过开放mito-KATPC 及下调活化的caspase-3蛋白的表达。     

脂蛋白脂肪酶单克隆抗体的制备及特征分析

用牛奶纯化的脂蛋白脂肪酶(LPL),一分子量为56KD,免疫Balb/c小鼠,利用杂交瘤技术,建立了30余株分泌抗朱LPL单克隆抗体的杂交瘤细胞。对其中2E_5、2G_(10)、6D_7、8D_2、7F_4进行特征分析表明:①其抗体类别均为IgG,亚类分别为IgG_1、TgG_(2b)、IgG_(2b)、IgG_1、IgG_1;②抗体效价(细胞培养上清)5×10~(-2)——2×10~(-3);③5种单抗可认为识别三个不同的抗原表位;④Western—blot及Dot—blot结果显示各单抗均能识别纯化的LPL,与人奶均无交叉反应,与牛奶显示不同的结合特性。     

Hallucinations and Delusions in Black Psychiatric Patients

A higher incidence of hallucinations has been previously reported among blacks, as compared to whites, in both psychiatric and nonpsychiatric populations. In contrast to these reports, which relied on hospital records, the present study utilized standardized research rating scales, and confirmed the above observation among schizophrenic patients. Some blacks, irrespective of diagnosis, probably experience a variety of non-schizophrenic hallucinations, may lead to erroneous diagnoses of schizophrenia. Current ignorance regarding the content of hallucinations in normal and non-schizophrenic, and in schizophrenic blacks is a source of diagnostic confusion which may have dire consequences for many individuals.     

Treatment of intracerebral arteriovenous malformations with isobutyl 2- cyanoacrylate: initial clinical experience

From November 1976 to September 1979, 46 patients with intracranial arteriovenous malformations or fistulas participated in a clinical study using isobutyl 2-cyanoacrylate (IBCA), with tantalum, for palliative or preoperative occlusion of the blood supply to the abnormalities. Although failure to obtain satisfactory position of a functioning microcatheter precluded deposition of IBCA 10 times, a total of 51 of a possible 62 feeding vessels were occluded with the tantalum-impregnated glue. The technique, results, and complications are discussed in light of the clinical follow-up, which varied from 12 to 48 months.     

螺内酯对阵发性心房颤动发作的影响

目的观察螺内酯对阵发性心房颤动(AF)的抑制作用及对心房结构和功能重构的影响。方法选择因Ⅲ度房室传导阻滞植入双腔起搏器的阵发性AF患者96例,分为两组,治疗组(48例)给予螺内酯20 mg/d,对照组(48例)不予螺内酯治疗。随访12个月,观察研究前后血浆醛固酮(Ald)浓度、AF发作情况、左房结构和功能变化。结果治疗组应用螺内酯12个月后Ald水平较前明显降低(5.30 ng/dl vs 7.70 ng/dl,P<0.01),AF发作次数及AF负荷明显减少,左房内径、左房最大容积、左房最小容积及左房收缩期前容积均减小,左房射血分数增高(P均<0.01)。与对照组相比,上述指标亦有显著差异(P均<0.05)。结论醛固酮受体拮抗剂——螺内酯能够抑制阵发性AF患者心房结构和功能的改变,减少AF发生率及持续时间。     

Larval habitats of mosquito fauna in Osogbo metropolis,Southwestern Nigeria

Objective

To determine the larval habitats of mosquito fauna and possible impact of land use/ land cover changes on the epidemiology of mosquito-borne diseases in Osogbo metropolis, Southwestern, Nigeria.

Methods

All accessible larval habitats were surveyed between May and September, 2011 in Osogbo metropolis while Land Use/ Land cover of the city was analyzed using 2 Lansat Multispectral Scanner satellite imagery of SPOT 1986 and LANDSAT TM 2009.

Results

A total of six species namely, Aedes aegypti, Aedes albopictus, Aedes vittatus, Anopheles gambiae complex, Culex quinquefasciatus and Eretmapodite chrysogaster were encountered during the study. The occurrence and contribution of disused tyres was significantly higher (P<0.05) than the other habitats encountered, while there were no significant differences in the contribution of gutters/run-offs, septic tanks/ drums, ground pools/open drains and discarded containers to the breeding of mosquitoes (P>0.05). The accessible land use/ land covered of the study area between 1986 and 2009 showed that the wet land coverage and settlement area increased from 0.19 to 9.09 hectare and 1.00 to 2.01 hectare respectively while the forest area decreased from 60.18 to 50.14 hectare.

Conclusion

The contribution of the habitats coupled with the increasing rate of flooded environment which could provide ample breeding sites for mosquitoes call for sustained environmental sanitation and management in Osogbo metropolis.     

鸡贫血病毒vp3基因的克隆和分子特征

目的 获得来源于浙江地区鸡组织中的鸡贫血病毒(CA)vp3基因并分析其变异情况.方法 根据GenBank登录的vp3序列设计特异引物,利用PCR方法从鸡组织中获得7个CAV的vp3基因克隆,将其克隆到载体进行测序.结果 40份鸡血清中共检出CAV阳性16份,阳性率40.0％.序列测定结果表明,vp3基因长366 bp,...     

乳腺纤维瘤组织差异蛋白质组的研究

目的:应用凝胶内差异显示电泳和质谱方法研究乳腺正常组织及纤维瘤组织蛋白质组的变化,探讨乳腺纤维瘤的发病机理。方法:提取乳腺正常组织及纤维瘤组织总蛋白,分别用Cy3或Cy5标记,与一个Cy2标记的内标等量混合后,上样于同一胶中进行电泳分离,经不同光激发下扫描得到不同样品的蛋白质组图谱。所获得的图谱经De Cyder 6.5软件进行分析,筛选表达量差异超过两倍的蛋白质进行质谱鉴定。结果:15个蛋白质在乳腺正常组织及纤维瘤组织中有显著差异。其中,T细胞受体、钙磷脂结合蛋白A2、a-B crystallin、肌球蛋白轻链\T盒转录因子3、CDC16蛋白、血红素α链、RAB14、烯醇酶1表达量增加,Cu,Zn-超氧化物歧化酶、肽基脯氨酸异构酶、转甲状腺素蛋白、转甲状腺素蛋白突变体、阻抑素表达降低。结论:这15个蛋白质可能与乳腺纤维瘤的发生发展相关,可以作为乳腺纤维瘤早期检测和治疗药物筛选的候选指标。     

A simple permutation‐based test of intermodal correspondence

Many key findings in neuroimaging studies involve similarities between brain maps, but statistical methods used to measure these findings have varied. Current state‐of‐the‐art methods involve comparing observed group‐level brain maps (after averaging intensities at each image location across multiple subjects) against spatial null models of these group‐level maps. However, these methods typically make strong and potentially unrealistic statistical assumptions, such as covariance stationarity. To address these issues, in this article we propose using subject‐level data and a classical permutation testing framework to test and assess similarities between brain maps. Our method is comparable to traditional permutation tests in that it involves randomly permuting subjects to generate a null distribution of intermodal correspondence statistics, which we compare to an observed statistic to estimate a p‐value. We apply and compare our method in simulated and real neuroimaging data from the Philadelphia Neurodevelopmental Cohort. We show that our method performs well for detecting relationships between modalities known to be strongly related (cortical thickness and sulcal depth), and it is conservative when an association would not be expected (cortical thickness and activation on the n‐back working memory task). Notably, our method is the most flexible and reliable for localizing intermodal relationships within subregions of the brain and allows for generalizable statistical inference.