Association between polycystic ovarian syndrome, overweight, and metabolic syndrome in adolescents

PurposePolycystic syndrome (PCOS) is associated with multiple metabolic abnormalities. Studies in the adolescent population are still limited and the results have been much different. The aim of this study was to investigate the association between PCOS, overweight, and metabolic syndrome in adolescents.Methods30 PCOS adolescents were randomly selected from a PCOS population with NIH 1990 criteria and 71 adolescents from the normal adolescents. Anthropometric, hormonal and metabolic parameters were evaluated in four sub-groups including obese and non-obese PCOS and obese and non-obese normal adolescents.ResultsThe prevalence of overweight and metabolic syndrome in adolescents with PCOS was 52% and 33.3% respectively vs 22.4% (P = 0.005) and 11.26% in control (normal) adolescents (P = 0.0001). Among all subjects, including obese and non-obese adolescents with or without PCOS, the prevalence of insulin resistance, hypercholesterolemia, central obesity, and metabolic syndrome was higher in obese PCOS with 61.5%, 46.2%, 53.8%, and 69.2%, respectively.ConclusionsObesity and IR are important risk factors for metabolic syndrome in PCOS. Considering the long-term health risks, it is necessary to identify metabolic disorders in adolescents with PCOS as early as possible.     

Pleural and pericardial effusions: rare presentations of brucellosis, Iran

We report a rare case of brucellosis presenting with pleural and pericardial effusions in a 35 year-old male rancher in Iran with fever and dyspnea. Such findings should prompt inclusion of brucellosis in the differential diagnosis in endemic areas.     

Health-related quality of life in patients with relapsing-remitting multiple sclerosis treated with subcutaneous interferon β-1a in Iran

Purpose: Multiple sclerosis (MS) requires long-term therapy and can affect many aspects of a patient's life, including quality of life. MS patients score lower on health-related quality of life (HRQoL) measures. The efficacy of subcutaneous interferon (IFN) β-1a has been extensively evaluated by using objective measures but its impact on HRQoL is currently unclear. In this observational study, we evaluated HRQoL of Iranian patients with relapsing-remitting MS (RRMS) treated with IFN β-1a by using short-form 36 (SF-36) and multiple sclerosis international quality of life (MusiQoL) questionnaires. Methods: Four hundred recruited RRMS patients were treated with human serum album free IFN β-1a for 1 year. Patients were required to fill in SF-36 and MusiQoL questionnaires at the first visit and at each follow-up visit. Expanded disability status scale (EDSS) evaluation was performed at baseline and at each visit. Comparisons in HRQoL between visits were calculated using Cohen's d effect size. The relationship between change in EDSS score and the score of each questionnaire was calculated using Pearson correlation coefficients. Results: Three-hundred and eighty three completed the study. Two-hundred and thirty nine were female. Mean (SD) age was 28.75 (±5.49). After 1 year, overall MusiQoL Index score effect size was ?0.16 and SF-36 physical component and mental component showed overall effect sizes of ?0.28 and ?0.53, respectively. Mean (range) EDSS change was 1 (1–4). Three-hundred and seventy four were clinically stable with mean (range) EDSS change of 0.1 (?2–0.5). Increase in EDSS was linked to a decrease in both MusiQoL and SF-36. Conclusion: We found that, HRQoL did not change significantly over the first year of therapy. Furthermore, decreases in HRQoL were inversely correlated with increases in EDSS score.     

Hematotoxic effects of direct infusion of crude diesel oil on juvenile great sturgeon Huso huso

Changes in the hematological and immunological parameters of fish due to anthropological pollutants, may lead to hematotoxic and immunotoxic effects. The objectives of current study were to determine the experimental effects of direct infusion of crude diesel oil on hematological and immunological features of juvenile great sturgeon Huso huso. During this toxicity test, juveniles of beluga were exposed to the acute doses (5, 10, 20, 40, 100, 500, and 1,000 ppm) of direct infusion crude diesel oil for 0, 48 h and 7 days. WBC, MCH, MCHC, and neutrophil in fish exposed to crude diesel oil for 48 h were significantly greater compared to the respective control groups and RBC, Hb, Ht, MCV, and lymphocyte were significantly lower than control groups (P?≤?0.05). Eosinophils did not vary significantly in the groups exposed to 48 h crude diesel oil compared to the respective control groups (P?>?0.05). Furthermore, 7-day exposures showed that WBC and Neutrophil were significantly greater compared to the respective control groups (P?≤?0.05) and MCV was depleted within the crude diesel oil adjacency.     

Pulsed-Field Gel Electrophoresis Analysis of Bordetella pertussis Isolates Circulating in Europe from 1998 to 2009

Between 1998 and 2009, Bordetella pertussis clinical isolates were collected during three periods, i.e., 1998 to 2001 (n = 102), 2004 to 2005 (n = 154), and 2007 to 2009 (n = 140), from nine countries with distinct vaccination programs, i.e., Denmark, Finland, France, Germany, The Netherlands, Norway, Poland, Sweden, and the United Kingdom. Pulsed-field gel electrophoresis (PFGE) analysis was performed according to standardized recommendations for epidemiological typing of B. pertussis. There were 81 different PFGE profiles, five of which (BpSR3, BpSR5, BpSR10, BpSR11, and BpSR12) were observed in 61% of the 396 isolates and shown to be predominant in almost all countries. The major profile, BpSR11, showed a decreasing trend from 25% to 30% in 1998 to 2005 to 13% in 2007 to 2009, and there were increases in BpSR3 and BpSR10 from 0% and 8% to 21% and 22%, respectively. One difference between these profiles is that BpSR11 contains isolates harboring the fim3-2 allele and BpSR3 and BpSR10 contain isolates harboring the fim3-1 allele. The total proportion of the five predominant profiles increased from 44% in 1998 to 2001 to 63% in 2004 to 2005 to 70% in 2007 to 2009. In conclusion, common PFGE profiles were identified in B. pertussis populations circulating in European countries with different vaccination programs and different vaccine coverages. These prevalent isolates contain the novel pertussis toxin promoter ptxP3 allele. However, there is evidence for diversifying selection between ptxP3 strains characterized by distinct PFGE profiles. This work shows that, even within a relatively short time span of 10 years, successful isolates which spread through Europe and cause large shifts in B. pertussis populations may emerge.     

Potential mechanisms of SARS-CoV-2 action on male gonadal function and fertility: Current status and future prospects

The novel coronavirus was recognised in December 2019 and caught humanity off guard. The virus employs the angiotensin-converting enzyme 2 (ACE2) receptor for entry into human cells. ACE2 is expressed on different organs, which is raising concern as to whether these organs can be infected by the virus or not. The testis appears to be an organ enriched with levels of ACE2, while the possible mechanisms of involvement of the male reproductive system by SARS-CoV-2 are not fully elucidated. The major focus of the present studies is on the short-term complications of the coronavirus and gains importance on studying the long-term effects, including the possible effects of the virus on the male reproductive system. The aim of this review was to provide new insights into different possible mechanisms of involvement of male gonads with SARS-CoV-2 including investigating the ACE2 axis in testis, hormonal alterations in patients with COVID-19, possible formation of anti-sperm antibodies (ASA) and subsequently immunological infertility as a complication of SARS-CoV-2 infection. Finally, we suggest measuring the sperm DNA fragmentation index (DFI) as a determiner of male fertility impairment in patients with COVID-19 along with other options such as sex-related hormones and semen analysis. Invasion of SARS-CoV-2 to the spermatogonia, Leydig cells and Sertoli cells can lead to sex hormonal alteration and impaired gonadal function. Once infected, changes in ACE2 signalling pathways followed by oxidative stress and inflammation could cause spermatogenesis failure, abnormal sperm motility, DNA fragmentation and male infertility.     

Levine-Sekhar grading system for prediction of the extent of resection of cranial base meningiomas revisited: study of 124 cases

INTRODUCTION: Skull base meningiomas comprise an intricate kingdom in neurological surgery. Due to their proximity to critical neurovascular structures, these tumours impose a cumbersome burden on the surgeon regarding surgical intervention and the clinical outcome. Preoperative prediction of the meningioma resectability will help the surgeon seek a rational result from surgery. This study tries to re-examine and promote the Levine-Sekhar (LS) grading system proposed to predict the resectability of basal meningiomas. PATIENTS AND METHODS: A retrospective study was performed on 124 eligible patients (90 female and 34 male) suffering from cranial base meningioma that had been operated on between April 1996 and February 2003. The patients were classified according to LS and our modified grading systems. The modified grading system deploys six groups of variables: optic apparatus involvement, cavernous sinus neural involvement, facial-auditory involvement, caudal cranial nerve dysfunction, data derived from imaging studies (multiple fossa involvement and/or vessel encasement), and history of previous radiosurgery. Each criterion scores 1 if present and the total score is the sum of scores obtained from the aforementioned criteria. RESULTS: Amongst 124 patients, 66 (52%) underwent gross total removal of the tumour. Regression and correlation analysis were performed for both LS (r(2) = 0.9683) and our modified grading systems (r(2) = 0.990) to evaluate the relationship of tumour grade versus the proportion of total resection. The correlations were significantly different (P < 0.01). CONCLUSION: Although the LS grading system is reported to be a good predictor of the extent of tumour resection, we believe that application of the six aforementioned variables will enhance the accuracy of this system, while preserving simplicity and communicability.