胎粪诱导肺损伤及rhSOD的抗炎抗氧化作用研究

目的:观察早期气管内给予抗氧化剂重组人超氧化物歧化酶(rhSOD)对胎粪诱导大鼠急性肺损伤的肺内脂质过氧化物和抗氧化物的产生及其炎症损伤的影响,探讨抗氧化干预的作用及机制.方法:32只雄性SD大鼠,由气管置管注入胎粪1 ml/kg复制胎粪肺损伤模型,然后随机分为:对照组和给予rhSOD 5 mg/kg、10 mg/kg、20 mg/kg组.24 h后取材,观察大鼠肺组织的湿/干重比、支气管肺泡灌洗液(BALF)蛋白含量及肺通透指数(BALF蛋白含量/血浆蛋白含量,PPI);利用比色法检测肺匀浆髓过氧化物酶(MPO)活性、一氧化氮(NO)含量及超氧化物歧化酶(SOD)活性;EIA法检测肺脂质过氧化物8-异前列腺烷含量.结果:与对照组比较,rhSOD各治疗组肺组织MPO活性显著降低(P分别<0.05、0.01和0.05),SOD活性显著增加(P均<0.05),NO含量显著下降(P分别<0.05、0.01和0.01),各治疗组经F检验,差异无显著性(P均>0.05);8-异前列腺烷含量下降(P分别<0.05、0.01和0.01),rhSOD 20 mg/kg组比rhSOD 5 mg/kg组更低(P=0.01),rhSOD 10 mg/kg组比rhSOD 5 mg/kg组略有下降,但差异无显著性(P=0.05);rhSOD 20 mg/kg组肺损伤病理评分明显减低(P<0.05),rhSOD 5 mg/kg、10 mg/kg组肺损伤病理评分略有下降,但差异无显著性(P均>0.05).各组肺组织湿/干重比、BALF蛋白含量、PPI的变化不明显(P均>0.05).结论:早期气管内给予单剂5、10或20 mg/kg rhSOD均可增加肺内SOD活性,减少脂质过氧化物产生,从而减轻胎粪诱导的大鼠肺炎症和过氧化损伤.     

Study on correlation of mammographic features、pathology and molecular bology of breast infiltrating ductal carcinoma

Objective To investigate the correlation among mammographic features、pathology and molecu-lar biology markers of breast infitrating ductal carcinoma(IDC) tissues.Methods The mammographic features of 93 cases with IDC confirmed by surgery and histopathology were analyzed retrospectively.The mastectomy specimens of the IDC were stained with immunohistochemistry,and the expression of ER、PR、C-erbB-2 were measured.The rela-tionship between the immunohistochemical pathologic results and mammographic features was analyzed.Results A-mong the 93 cases of IDC,ER positive expression was positively correlated with the spiculate margin of breast cancer (P<0.05);C-erbB-2 positive expression was positively correlated(P<0.05).Moreover,ER and PR positive expres-sion showed a significant inverse correlation with the calcifying of breast cancer(P>0.05);ER and PR positive ex-pression was positively correlated with the transfer of lymph(P<0.05);there existed correlation between the positive expression of C-erbB-2 and lymphatic metastasis and pathohistology grade(P<0.05).Conclusion There was a pos-itive correlation among IDC mammography、pathology and the abnormal value of ER、PR and C-erbB-2.The X-ray mammography could reflect the diagnosis value of ER、PR and C-erbB-2 roughly,and thadpractical value in determi-ning prognoses and endocrinotherapy.