Studies on arsenictrioxide induced human pulmonary adenocarcinoma GLC-82 cell apoptosis and its molecularmechanisms

】 ObjectiveTo study thebiological effect of As₂O₃ on human pulmonary adenocarcinoma GLC-82cells and its mechanisms. MethodsMTT reduction assay,morphologyinvestigation,flowcytometric analysis,DNA gel electrophoresis and TUNEL,RT-PCR,Northernblot, Western blot methods were perfomed. ResultsAs₂O₃inhibited the growth of GLC-82 cell line. The cells treated with As₂O₃showed a typical apoptotic morphology and hypodiploid peak before G1 phase. DNA of treatedGLC-82 cells appeared a ladder pattern characteristic of apoptosis. In situ cell deathdetection analysis also revealed the DNA fragmentation. Besides,As₂O₃inhibited c-myc gene expression and increased p53 and p16 gene expression. ConclusionAs₂O₃can induce GLC-82 cell apoptosis mainly with regulation of c-myc,p53 and p16 geneexpression.     

宁波市某区４５～６４岁人群空腹血糖和糖耐量特征分析

摘要：目的　了解宁波市某区４５～６４岁人群空腹血糖和糖耐量分布特征,为中年人群的糖尿病防治提供依
据。方法　通过横断面调查方法,分层整群随机抽取宁波市某区４５～６４岁中年人群,进行空腹血糖和糖耐
量试验,分析流行病学特征。采用χ
２ 检验、趋势χ
２ 检验、方差分析、调整率等进行统计描述及检验。结
果　共调查４５～６４ 岁人群６１７０ 人, 空腹血糖平均值为５．２９ ｍｍｏｌ／Ｌ, 男性高于女性（犉＝９．３５,犘＝
０．００２）。糖耐量２ｈ血糖平均值为６．４１ｍｍｏｌ／Ｌ,女性高于男性（犉＝１８．６７,犘＝０．０００）。联合筛查结果为
糖尿病检出率５．２５％, 糖尿病前期检出率１７．５９％。仅检测空腹血糖, 中年人群糖尿病的漏检率为
４５．３７％ （１４７／３２４）,糖尿病前期的漏检率为７５．８５％ （８２３／１０８５）。结论　糖尿病对中年人群的影响不容
忽视,加做糖耐量试验对减少糖尿病和糖尿病前期者的漏诊意义重大。
关键词：空腹血糖;糖耐量试验;糖尿病;人群
中图分类号：Ｒ５８７．１　　文献标识码：Ａ　　文章编号：１００９ ６６３９ （２０１４）０５ ０４８６ ０４