荧光定量PCR测定端粒长度

目的 应用实时荧光定量聚合酶链式反应(Q-PCR)方法测定端粒长度.方法 选取9种人类细胞株,提取基因组DNA,采用Q-PCR方法测定相对T/S比率,DNA印迹法测定末端限制性片段(TRF)长度,进行二者之间的相关性分析.结果 定量PCR测定端粒长度相对T/S比率为0.68±0.57,DNA印迹法测量平均TRF值为8.57±2.34,两种方法测定结果的相关性分析R2=0.7807(P＜0.01).结论 采用荧光定量PCR方法测量端粒长度具有重复性好、省时、简便、可靠的特点,可高通量处理大量样品.     

中医妇科663株解脲脲原体药敏结果分析

目的了解广东省中山地区中医妇科解脲脲原体（UU）对9种抗生素的药物敏感情况。方法采集我院妇科患者的泌尿生殖道分泌物，用珠海丽珠试剂有限公司生产的试剂盒进行检测。结果UU对克拉霉素敏感率最高，为92．2％；对氧氟沙星敏感率较低，仅为28．8％。结论克拉霉素可作为目前本地区中医妇科治疗支原体感染的首选药物。     

血透患者颈内静脉长期留置导管应用

1　资料与方法1.1　研究对象　慢性血透患者 6 5例 ,其中男性 33例 ,女性 32例。原发病慢性肾炎 48例 ,糖尿病肾病 10例 ,肾移植后失功 3例 ,其它 4例。年龄范围 14～ 77岁 (平均 5 8± 13岁 )。已行血透治疗 0～ 15年。1.2　插管原因　内瘘狭窄、闭塞或失败 34例 ,自身血管条件差无法行内瘘手术 2 3例 ,害怕静脉穿刺要求行长期留置导管 8例 ,其中 2例患者因长期留置导管不畅分别重新置管 2次和 3次。1.3　插管方法　颈内静脉临时留置管原穿刺点直接改为长期留置导管 18次 ,经右侧 (或左侧 )颈部胸锁乳突肌下三角为穿刺点 5 0次 ,长期导管出…     

血液透析治疗毒蛇咬伤致急性肾功能衰竭九例

我们总结9例利用血液透析治疗毒蛇咬伤所致的急性肾功能衰竭(ARF)患者,报道如下。 临床资料 1.一般资料;9例中男5例,女4例,年龄29～63岁,其中7例明确为蝮蛇咬伤,2例未辨明蛇种类。受伤部位为手指、手掌或足背。     

国产Amplatzer封堵器治疗先心病的初步临床研究

目的：报道用国产Amplatzer封堵器治疗先天性心脏病（先心病）的临床经验。方法：82例先心病患者经超声和心血管造影诊断后，分别应用3种类型的国产Amplatzer封堵器封堵动脉导管未闭（PDA）、房间隔缺损（ASD）和室间隔缺损（VSD），观察其安全性、术中和术后近期疗效。结果：PDA28例（漏斗型23例，管型5例），直径4～11mm，封堵成功率100％。继发孔型ASD34例，直径8～32mm，封堵成功率97％。VSD20例，均为膜周部，直径3～10mm，封堵成功率100％，术后即刻造影均成功，无明显并发症。结论：应用国产Amplatzer封堵器治疗先心病是安全、有效的。     

维持性血液透析患者微炎症状态的认识与防治

近年来，尽管对尿毒症毒素的认识不断深入以及血液净化技术已有长足的发展，终末期。肾病(ESRD)患者的预后仍然不够理想。经过治疗的ESRD患者的死亡率仍高达25％，预期寿命比正常人群少20～25年。其中最主要的死因是心、脑血管并发症，占全部死因的40％～50％，是普通人群的20～100倍，而加速进展的动脉粥样硬化是最受关注的原因之一。尸检和冠脉造影发现，     

基于慢病毒的SHARP-2特异的RNA干扰延长肾移植受者大鼠存活时间

Objective To investigate if rat enhancer of split- and hairy-related protein-2 (SHARP-2) short hairpin RNA interference (shRNAi) prolongs the survival time of rat kidney transplant recipients. Methods Gene recombinant procedures, transfection and co-transfection were carried out to introduce short hairpin RNA interference sequences target for SHARP-2 into 3rd generation self-inactivated lentiviral-ViraPower packaging mix. Limiting dilution method was used for viral titration. Real-time PCR was employed for quantification of gene expression. Rat kidney transplantation was utilized to investigate the effect of SHARP-2 gene silence on the recipient survival. Results A lentiviral-based shRNAi construct LV-SHARP-2iC showed 84% SHARP-2 gene silence efficiency in normal rat kidney cells. At multiplicity of infection 20, 57% T cells could be transfected by lentivirus with spinoculation method. In activated T cells, SHARP-2 g ene silence resulted in 61.3% and 68.7% reduction of intedeukin 2 (IL-2) and interferon γ (IFN-γ) gene expression. When donor kidney was perfused with 5×107 TU LV-SHARP-2iC, the median survival time prolonged for 4-5 days as compared to blank and scramble control groups. Conclusions A recombinant lentivirus LV-SHARP-2iC that effectively silence SHARP-2 gene expression is constructed successfully, leading to the inhibition of IL-2 and IFN-γ. LV-SHARP-2iC treatment can prolong the survival time of rat kidney transplant recipients.     

基于慢病毒的SHARP-2特异的RNA干扰延长肾移植受者大鼠存活时间

Objective To investigate if rat enhancer of split- and hairy-related protein-2 (SHARP-2) short hairpin RNA interference (shRNAi) prolongs the survival time of rat kidney transplant recipients. Methods Gene recombinant procedures, transfection and co-transfection were carried out to introduce short hairpin RNA interference sequences target for SHARP-2 into 3rd generation self-inactivated lentiviral-ViraPower packaging mix. Limiting dilution method was used for viral titration. Real-time PCR was employed for quantification of gene expression. Rat kidney transplantation was utilized to investigate the effect of SHARP-2 gene silence on the recipient survival. Results A lentiviral-based shRNAi construct LV-SHARP-2iC showed 84% SHARP-2 gene silence efficiency in normal rat kidney cells. At multiplicity of infection 20, 57% T cells could be transfected by lentivirus with spinoculation method. In activated T cells, SHARP-2 g ene silence resulted in 61.3% and 68.7% reduction of intedeukin 2 (IL-2) and interferon γ (IFN-γ) gene expression. When donor kidney was perfused with 5×107 TU LV-SHARP-2iC, the median survival time prolonged for 4-5 days as compared to blank and scramble control groups. Conclusions A recombinant lentivirus LV-SHARP-2iC that effectively silence SHARP-2 gene expression is constructed successfully, leading to the inhibition of IL-2 and IFN-γ. LV-SHARP-2iC treatment can prolong the survival time of rat kidney transplant recipients.     

基因芯片在肾移植中的应用进展

基因芯片是一种综合了寡核苷酸高密度空间合成技术的核酸分子杂交分析技术，可同时、快速、高效、高通量分析生物信息。本文综述了基因芯片在肾移植组织配型、移植免疫机制、移植术后感染的诊断以及敏感药物筛选、抗排斥药物作用机制方面的研究进展。     

基于慢病毒的SHARP-2特异的RNA干扰延长肾移植受者大鼠存活时间

Objective To investigate if rat enhancer of split- and hairy-related protein-2 (SHARP-2) short hairpin RNA interference (shRNAi) prolongs the survival time of rat kidney transplant recipients. Methods Gene recombinant procedures, transfection and co-transfection were carried out to introduce short hairpin RNA interference sequences target for SHARP-2 into 3rd generation self-inactivated lentiviral-ViraPower packaging mix. Limiting dilution method was used for viral titration. Real-time PCR was employed for quantification of gene expression. Rat kidney transplantation was utilized to investigate the effect of SHARP-2 gene silence on the recipient survival. Results A lentiviral-based shRNAi construct LV-SHARP-2iC showed 84% SHARP-2 gene silence efficiency in normal rat kidney cells. At multiplicity of infection 20, 57% T cells could be transfected by lentivirus with spinoculation method. In activated T cells, SHARP-2 g ene silence resulted in 61.3% and 68.7% reduction of intedeukin 2 (IL-2) and interferon γ (IFN-γ) gene expression. When donor kidney was perfused with 5×107 TU LV-SHARP-2iC, the median survival time prolonged for 4-5 days as compared to blank and scramble control groups. Conclusions A recombinant lentivirus LV-SHARP-2iC that effectively silence SHARP-2 gene expression is constructed successfully, leading to the inhibition of IL-2 and IFN-γ. LV-SHARP-2iC treatment can prolong the survival time of rat kidney transplant recipients.