消渴停对胰岛B细胞凋亡及相关基因Bcl-2、Fas蛋白表达的影响

目的：探讨消渴停降血糖的疗效和机理。方法：采用链脲佐菌素（STZ）腹腔注射所致糖尿病大鼠模型，应用HE染色、免疫组化、原位杂交和透射电镜技术。观察消渴停对胰岛B细胞凋亡的影响。结果：消渴停具有预防胰岛B细胞凋亡，刺激胰岛B细胞分泌胰岛素和抑制Fas蛋白表达的作用。结论：消渴停具有抑制Fas基因诱导胰岛B细胞凋亡的作用。     

慢性阻塞性肺疾病稳定期从气论治

慢性阻塞性肺疾病是一种具有气流受限特征的疾病,气流受限不完全可逆,呈进行性发展.目前西医治疗只能改善肺功能,不能阻止气道阻塞的进展和气道重塑.近年来运用传统中医药治疗COPD取得显著成效,尤其是对稳定期,能够阻止病情进展、改善肺功能、增强机体免疫力、改善气道高反应性和气道重塑,预防和治疗并发症等.     

动态平衡观在中西医生理学中的体现

动态平衡体现在结构、功能、量的协同作用上；也体现在人体精神意识及自身与外界环境关系上，即通过机体自身调节达成人体内、外环境的统一，维持或恢复协调状态。动态平衡观是中西医学生理学的共性，为研究中西医结合医学奠定基础。     

114例微创切口心胸外科手术分析

目的：探讨微创切口在胸心外科手术中的应用。方法：回顾性分析随机选用的114例心胸外科微创切口手术（微创组）资料，并与同期完成的150例常规手术（对照组）相比较。结果：两组在术中出血量、术后疼痛缓解时间、术后引流量、术后主要并发症等方面差异有显著性（P＜0.01)，畸形愈合发生率两组差异无显著性。结论：微创切口进行心胸外科手术具有多方面的优势。     

Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway

Objective:To study the immunologic function of dendritic cells(DCs) cultured in two kinds of hepatoma cell line’s supernatant and the enhancing effects of carboxymethylpachymaran(CMP) on DCs.Methods:DCs were harvested after stimulation by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin(IL)-4 from umbilical cord blood using density-gradient centrifugation method. Cultured supernatant of two hepatoma cell lines(HepG2 and HepG2.2.15) were collected for condition medium(CM) according to a volume ratio of supernatant to incomplete RPMI-1640 medium,which was 3:1.CMP was dissolved in incomplete RPMI-1640 medium.Experimental groups were divided according to the culture medium,either CM or with CMP in it.DCs subsets CD83,CD86,CD1a,and d-related human leukocyte antigens(HLA-DR) were analyzed by flow cytometry.The proliferation ability of allogeneic T cells in mixed lymphocyte reaction(MLR) stimulated by DCs was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) analysis.IL-12p70,interferon-γ(IFN-γ),and nuclear factor k B(NF- k B) were detected by enzyme-linked immunosorbent assay analysis.Results:The proliferation of lymphocytes and secreting level of IL-12 and expression of phenotype of DCs cultured in two kinds of CM were lower than those of normal group(P<0.01).Compared with the normal group,groups treated with CMP showed a higher expression level of DCs subsets,lymphocyte reproductive activity,as well as IL-12 and IFN-γsecretion levels.Groups treated with CMP also demonstrated higher levels of DCs phenotype expression and IL-12 and IFN-γsecretion in supernatant of MLR and higher lymphocyte reproductive activity compared with CM group(P<0.05).Compared with the normal group,the expression level of NF-k B in DCs nuclear was higher in CMP groups but lower in two CM groups(P<0.05).After CMP was added,the NF-κB expression levels of two CM groups were increased compared with levels before CMP was added(P<0.05).However,there was no significant difference between the two CM groups(P>0.05).Conclusions:Two kinds of hepatoma cell line’s supernatant can inhibit the immunologic function of DCs.This suppressive effect may be related to the inhibition of NF-κB/Rel pathway.CMP may up-regulate the DCs function by activating the NF-κB/Rel pathway.     

可拓学在中医药研究中应用方法探讨

中医药是我国劳动人民在几千年来同疾病作斗争中的经验总结，曾为中华民族的繁衍作出过重大贡献，现仍为广大人民群众所喜爱，并在我国医疗卫生中占有很大比重。     

参苓白术散对肠道菌群及免疫调节作用的探讨

参苓白术散是治疗脾虚泄泻的常用方,对调节肠道菌群、增强机体免疫力均有明显作用,故可明确参苓白术散治疗脾虚泄泻的机制与肠道菌群以及机体免疫平衡相关,为临床使用参苓白术散治疗脾虚泄泻提供参考依据。     

将可拓学应用于外感病病因研究的探讨

中医外感病是多因素作用的结果,用传统方法很难在病因研究上取得突破性进展。我们引入了专门用于解决矛盾问题的可拓学来分析致病因素与外感病以及各因素之间的相互关系。本文简单介绍了可拓学,就可拓学对外感病病因研究的指导作用进行了分析,还展望了医院信息系统。     

习惯性落枕的登山运动疗法机制浅析

从颈部解剖结构、卧位时的颈椎受力情况、颈部肌肉对颈椎额状面矢状面平衡与稳定的作用、功能锻炼对颈椎稳定的作用等四个方面论述颈椎的稳定机理,分析习惯性落枕的病因病机,提出防治习惯性落枕的原则,并观察19例反复发作的习惯性落枕患者3个月登山运动后的疗效,说明登山运动疗法对本病治疗有良效,并全面分析登山运动防治习惯性落枕的生物学原理。     

调肝方药对束缚应激大鼠神经内分泌免疫功能的调节作用

目的:探讨调肝方药对束缚应激大鼠神经内分泌免疫功能的调查作用。方法:以束缚制动作为应激原复制应激反应大鼠模型,采用放射免疫法检测模型大鼠下丘脑-垂体-肾上腺轴功能,同时检测模型大鼠有关免疫功能的变化,并观察调肝方药的调节作用。结果:束缚应激大鼠下丘脑-垂体-肾上腺轴(HPAA)兴奋亢进(P＜0.01或P＜0.05);脾淋巴细胞增殖反应降低(P＜0.01),腹腔巨噬细胞(MΦ)释放H₂O₂功能下降(P＜0.01)。而调肝方药能抑制HPAA的兴奋性,提高大鼠的免疫机能。结论:调肝方药对束缚应激所引起的神经内分泌免疫功能的紊乱具有一定的调节作用。