应用聚合酶链反应快速诊断结核性脑膜炎

应用聚合酶链反应快速诊断结核性脑膜炎中国医科大学第二临床学院（１１０００３）郭津津，房荣庄，郭阳朝阳市结核病防治院梁志津沈阳市第一结核病院张革平结核性脑膜炎的早期诊断有赖于从脑脊液中检出结核杆菌，传统的涂片抗酸染色法阳性率低，特异性差。常规培养法需８...     

Additive effects of earthworm extract with HpD-laser on the enhancement of reactive oxygen production and the inhibition of[~3H]—thymidine.uptake in mouse ascitic tumor cells

The effects of combined use of earthworm extract(912)and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S_(180) tumor cells were studied throughchemiluminescence measurement and[~3H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[~3H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O_2~ and H_2O_2 in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.     

简易测量软性接触镜屈光度

随着角膜接触镜的应用,有许多问题相应产生。目前对软性角膜接触镜度数的测量还存在一定困难。生产厂家可使用价格昂贵的仪器,但不能普及应用。因此,我们就简易测量软性角膜接触镜屈光度数的方法介绍如下:首先测量配戴者的裸眼视力,然后配戴软性角膜接触镜测出最佳矫正视力,例如配戴者是近视眼时,就使用凸球镜逐渐增加屈光度数,直至配戴者的视力达到裸眼视力为止,记下凸球镜的度数(远视者使用凹球镜)。普通眼镜一般距离角膜顶点12mm,而角膜接触镜紧贴在角膜上,故在眼部实际矫正屈光力不同。其计算方法为F_0=(Fs)/(1-dFs) F_0=软性角膜接触镜屈光度数,Fs=测量后的凸、凹球镜度数(代入公式后改     

纤维连接蛋白和层粘连蛋白对瘤细胞移动性的影响

用琼脂糖滴扩散法分别观察在培养基内加入不同浓度的纤维连接蛋白（ｆｉｂｒｏｎｅｃｔｉｎ，Ｆｎ）和层粘连蛋白（Ｌａｍｉｎｉｎ，Ｌｎ）及地塞米松后，培养的人肺巨细胞癌细胞和肺腺癌细胞体外移动性的改变；同时用考马斯蓝Ｒ－２５０染色显示瘤细胞内微丝结构的变化。结果显示加入外源性Ｆｎ和Ｌｎ后，两株瘤细胞的移动性均增强，胞浆内近胞膜周围的微丝增多；地塞米松处理后，瘤细胞内Ｆｎ合成增加，细胞移动性降低，胞浆内微丝增多，杂乱弥漫分布。结果表明瘤细胞移动性的变化与细胞内微丝结构的变化有关。实验还观察到内源性Ｌｎ阳性的肺巨细胞瘤细胞的体外移动能力大于Ｌｎ阴性的肺腺癌细胞。     

肾移植受者血清巨细胞病毒IgE和IgA抗体检测

采用间接ＥＬＩＳＡ检测２３名肾移植受者血清巨细胞病毒（ＣＭＶ）抗体，共检出１８名（７８％）活动性ＣＭＶ感染，其中１０名（４４％）为原发性感染。结果证实ＣＭＶ－ＩｇＥ和－ＩｇＡ具有较好的血清学诊断价值，优于ＣＭＶ－ＩｇＭ。     

豁痰通络法治疗胸痹的临床研究

目的：探讨豁痰通络法治疗胸痹的作用。方法：选择具有豁痰通络作用的自拟豁痰通络方治疗60例冠心病心绞痛患者，观察治疗前后心绞痛、伴随症状、心电图及血液流变学指标的变化，判断其临床效果。结果：60例患者心绞痛及伴随症状的总有效率分别为86．67％和90％，治疗前后心电图和血液流变学指标有一定改善。结论：提示豁痰通络法是治疗胸痹的有效方法。     

老年人前臂离断再植一例

患者女,83岁,农民.因左前臂电锯伤2 h急诊入院.全身检查:生命体征正常.专科检查:左前臂腕上5 cm处横形离断,仅桡背侧有少许皮肤及拇伸肌腱相连;伤口组织挫伤严重;桡、尺骨粉碎性骨折,骨缺损约2cm.     

新生儿呼吸衰竭应用机械通气治疗中肺不张的预防及护理

新生儿呼吸衰竭应用机械通气治疗中肺不张的预防及护理２１０００８南京市儿童医院郭岚峰，徐英美肺不张是新生儿呼吸衰竭应用机械通气治疗的常见并发症。我院过去的发生率高达６１．５％。近几年来我们在治疗与护理上采取了一些改进措施，统计了１９９０年１月～８月在Ｎ...     

胆囊切除时肝活检70例体会

胆囊切除时肝活检７０例体会郭盖章我院６年来行胆囊切除术６５９例，其中行肝活检７０例，占１０．２％。病理证实均有不同程度的肝脏病变。本组男性４４例，女性２６例，年龄１６～８２岁，平均５０．７岁。既往史中胃病２８例，胆绞痛１７例，肝炎１７例，心绞痛６例，...