老年糖尿病酮症酸中毒患者IL-6和氧化应激的变化

目的: 探讨老年糖尿病酮症酸中毒(DKA)患者白细胞介素6(IL-6)和氧化应激的变化.方法:检测老年糖尿病酮症酸中毒患者治疗前后血IL6、 8异前列腺素F-2α(8-isoprostaglandinF2α,8isoPGF2α)水平、超氧化物歧化酶(SOD）活性、总抗氧化能力(TAC)和丙二醛(MDA）含量.结果:在DKA患者SOD、 TAC显著低于对照组(P<0.05), 而IL6、 MDA和8isoPGF2α显著高于对照组(P<0.05); DKA患者治疗后的SOD和TAC显著高于治疗前患者(P<0.05), 而IL-6、 MDA和8isoPGF2α显著低于治疗前患者(P<0.05).DKA患者治疗前IL6与8isoPGF2α呈显著性正相关(r=0.33, P<0.05）, 治疗后IL-6与SOD呈显著性负相关(r=-0.36, P<0.05）.DKA患者治疗前后IL-6与MDA均呈显著性正相关(r=0.38, 0.41, P<0.05）.结论: DKA患者血清IL-6水平明显升高, 且与氧化应激有关.     

高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.     

2型糖尿病肾病患者血清视黄醇结合蛋白-4变化与氧化应激的关系

目的探讨2型糖尿病肾病(DN)患者血清视黄醇结合蛋白-4(RBP4)变化及其与氧化应激的关系。方法检测DN患者血清RBP4、血浆8-异前列腺素F2α(8-iso-PGF2α)、血浆超氧化物歧化酶(SOD)活性、总抗氧化能力(TAC)和丙二醛(MDA)含量。结果在单纯糖尿病(DM)组8-iso-PGF2α明显高于对照组(P0.01),SOD和TAC明显低于对照组(P0.01);早期DN组和临床DN组患者8-iso-PGF2α、MDA和血清RBP4明显高于对照组(P0.05,P0.01);临床DN组患者8-iso-PGF2α和血清RBP4明显高于单纯DM组和早期DN组(P0.05,P0.01)。DM患者血清RBP4与腰围/臀围(WHR)、空腹血糖(FBG)、收缩压(SBP)、尿白蛋白排泄率(UAER)和8-iso-PGF2α显著性正相关(分别为r=0.39、0.37、0.33、0.39、0.41,P0.05),与血肌酐(Cr)和SOD呈显著性负相关(分别为r=-0.28、-0.35,P0.05)。多元逐步回归分析显示WHR、UAER和8-iso-PGF2α均为影响血清RBP4水平的显著因素。结论DN患者血清RBP4水平增加与WHR、UAER和氧化应激有关。     

肥胖患者氧化应激和脂肪细胞因子变化

目的:研究男性单纯肥胖患者氧化应激(OS)和脂肪细胞因子变化及相互关系.方法:测定42例男性肥胖患者和32例健康非肥胖男性个体的血8-异前列腺素F2α(8-iso-PCF2α)、血浆超氧化物歧化酶(SOD)、血清丙二醛(MDA)、脂联素、瘦素、抵抗素、人肿瘤坏死因子可溶性受体1(TNF-R1)、白介素-1β(IL-1β)、白介素-6(IL-6).结果:肥胖患者8-iso-PGF2α、MDA、瘦素、TNF-RI、IL-1β和IL-6均明显高于正常对照组(P<0.05,P<0.01),脂联素和SOD均明显低于正常对照组(P<0.05,P<0.01),2组抵抗素水平无统计学意义(P>0.05).肥胖组相关分析:8-iso-PGF2α与BMI(r=0.54,P<0.05)呈正相关,与脂联素(r=-0.56,P<0.05)呈负相关,瘦素与体脂(r=-0.53,P<0.05)呈负相关,脂联素与LDL(r=-0.54,P<0.05)和IL-6(r=-0.41,P<0.05)呈负相关.多元逐步回归分析:脂联素和IL-6是影响肥胖患者OS变化的主要因素.结论:男性单纯肥胖患者存在OS和脂肪细胞因子变化,脂肪细胞因子与OS存在明显的相关性.     

高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.     

糖尿病高糖危象患者氧化应激与细胞黏附因子变化的关系

血浆细胞黏附分子(CAM)增加被认为是全身内皮细胞功能和白细胞损害或激活的标志,也是早期动脉粥样硬化的分子标记.自由基增加与糖尿病大血管和微血管并发症存在明显相关性,同时,CAM与细胞所处的氧化还原状态密切相关.     

高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.     

高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.     

高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.