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抗CD71人-鼠嵌合抗体的体外抗肿瘤效应研究 总被引:1,自引:0,他引:1
目的 对抗CD71人-鼠嵌合抗体体外抗肿瘤效应进行研究。方法 以表达CD71分子的3种人类肿瘤细胞(K562,CEM和SMMC-7721)为靶细胞,以正常人外周血单个核细胞(PBMC)为效应细胞,在效/靶细胞比为50:1的条件下,测定嵌合抗体依赖性细胞介导的细胞毒效应(ADCC);以CEM和SMMC-7721为靶细胞,在有新鲜补体存在下,测定嵌合抗体的补体依赖性细胞毒效应(CDC)。结果 抗CD71人-鼠嵌合抗体(D2C)能够通过识别CD71分子,并通过人抗体Fe段介导ADCC。在有新鲜补体存在下,介导CDC。结论 抗CD71人-鼠嵌合抗体对CD71^ 肿瘤细胞可介导ADCC和CDC。 相似文献
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可溶性人干细胞因子与EGFP融合基因的构建及真核表达 总被引:1,自引:0,他引:1
目的 可溶性人干细胞因子 (SCF)膜外活性区与增强型绿色荧光蛋白 (EGFP)融合基因表达载体的构建及其表达。方法 应用基因工程技术构建重组载体 ,电穿孔转染CHO K1细胞株 ,荧光分光光度计检测上清液中融合蛋白的表达 ,流式细胞仪检测其活性。结果 融合基因在瞬时转染的真核细胞中获得表达 ,并分泌至上清 ,且SCF EGFP融合蛋白中分子标签EGFP未影响可溶性人SCF膜外活性区的结构及其生物学活性。结论 可溶性人干细胞因子与EGFP融合基因载体构建成功 ,表达的融合蛋白具有生物活性 ,为进一步研究SCF对造血干细胞的生物学作用奠定了基础。 相似文献
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邵静芳 《中国饮食卫生与健康》2005,3(5):48-49
2004年7月18日,我县朱家镇富家村发生一起疑似食物中毒事件。发病62人,经流行病学调查及实验室检查证实为一起由金黄色葡萄球菌污染豆制品引起的食物中毒事件。现将结果报告如下。 相似文献
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MonoclonalantibodiesagainstCD4molecule(anti-CD4McAb)ofhumanTlymphocyteshavebeensuccessfullyusedforthetreatmentofautoimmunediseasesandpreventionoforgantransplantationrejectioninhuman[1'Zj.However,murinemonoclonalantibodies(Ahs)haveimmunogenicityonhumanandproduceneutralizinghumananti-mouseantibodies(HAMA).ToreducetheimmunogenicityofmurineMcAbinhuman,chimericAhshavebeenproduced,inwhichtheVregionsofthemouseantibodywiththedesiredspecificityarecombinedwithhumanCregions["4j.Inthisstudy,anti-C… 相似文献
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Construction, Expression and In Vitro Biological Behaviors of Ig scFv Fragment in Patients with Chronic B Cell Leukemia 总被引:1,自引:0,他引:1
The clonotypic surface Ig receptor(SmIg)ex-pressed by malignant Bcells,idiotype,is a tumor-specific antigen and an attractive target for activei mmunotherapy.This kind of SmIg can be pro-cessed and presentedinthe context of MHCclass Iand classⅡmolecules onthe surface of the tumor.It has been shown that Id vaccination inducestumor-specific T cell responses in patients with Bcell malignancies.Such antigenic epitopes mayserve as candidates for novel peptide-vaccine strat-egies,and as tools … 相似文献
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雷公藤甲素(Triptalide)系从雷公藤中提取到的一种二萜类单体成份,其中含有治疗类风湿性关 相似文献
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In this study, the isolation, purification and differentiation of tumor-lnflltratlng lymphocytes (TIL) from 6 fresh osteosarcoma specimens were achieved by discontinuous density gradient centrifugation. One specimen of the osteosarcoma TIL were enlarged in IL-2 for long time in vitro, reaching 28 days and their cytolytic activity against different tumor cell lines was Investigated. The experimental results indicated that the preparation of osteosarcoma TIL adopted by the mechanical means was simple, having higher purifity, keeping higher effects on killing NK- sensitive tumor cell lines and NK-insensitive tumor cell lines as well as rapid proliferation in vitro cultured in IL-2. 相似文献