大鼠睡眠剥夺与p38MAPK信号通路的相关性研究

[目的]探讨p38有丝分裂原活化蛋白激酶(p38MAPK)信号通路在大鼠睡眠剥夺中的作用及与炎症因子的关系。[方法]将40只雄性Wistar大鼠随机分为对照组、大平台组、小平台睡眠剥夺组、抑制剂组,每组10只。采用免疫组化观察大鼠海马区IL-1和磷酸化p38MAPK的表达,同时利用水迷宫检测大鼠认知功能。[结果]与对照组和大平台组对比,睡眠剥夺组IL-1β与磷酸化p38MAPK表达明显上调(P﹤0.05),抑制剂组显著下降(P﹤0.05)。认知功能评价中睡眠剥夺组明显低于对照组和大平台组(P﹤0.05),抑制剂治疗组明显改善(P﹤0.05)。[结论]p38MPAK信号转导通路参与了大鼠睡眠剥夺的过程,且可引起炎症因子的释放,该作用可影响大鼠认知功能。     

注射用泮托拉唑钠用药注意事项

注射用泮托拉唑钠为白色或类白色疏松块状物或粉末.本品通过特异性地作用于胃黏膜壁细胞,降低壁细胞中的H+/K+ATP酶的活性,从而抑制胃酸的分泌.适用于十二指肠溃疡、胃溃疡、急性胃黏膜病变等.     

缺血性脑卒中患者执行功能障碍影响因素研究

目的探讨缺血性脑卒中患者执行功能(EF)损伤及其影响因素,为卒中后EF障碍患者提供防控依据。方法采用威斯康星卡片测验系统(WCST)对480例稳定期缺血性脑卒中患者的EF进行评测,以同期在该院进行体格检查的健康人为对照组,结合一般临床资料分析相关因素。结果缺血性脑卒中患者WCST的持续应答数、完成分类数、正确应答数、错误应答数、持续性错误数、非持续性错误数评分(16.51±5.58、5.51±0.64、65.88±6.31、31.46±16.05、14.14±5.41、16.46±7.76)明显差于对照组(24.28±12.58、3.88±1.37、58.32±5.70、43.43±18.99、21.13±5.71、23.99±14.45);单因素方差分析不同年龄、教育程度、吸烟指数、饮酒量、梗死体积、梗死部位以及不同合并症患者WCST评分存在差异;多因素分析卒中患者WCST的各项评分与性别无关,与年龄、教育程度、吸烟指数、饮酒量、梗死体积、梗死部位以及合并症(OR值分别为:1.23、0.90、1.85、1.21、1.31、3.76、2.52)等有关。结论缺血性脑卒中患者存在EF障碍,梗死面积大、梗死部位在额叶、合并症有高血压糖尿病等、大量吸烟、大量饮酒、年龄大为缺血性脑卒中EF障碍发病的独立危险因素,高教育程度、经常锻炼为缺血性脑卒中EF障碍发病的保护因素。     

参芎化瘀胶囊通过磷脂酰肌醇激酶/蛋白激酶B信号通路抑制全脑缺血大鼠神经细胞凋亡

目的:探讨参芎化瘀胶囊对大鼠脑缺血/再灌注损伤神经细胞凋亡的影响.方法:雄性SD大鼠被分成假手术组,模型组,参芎化瘀胶囊高、低剂量组,以改良的Pulsineli 4血管阻断(4-VO)法制作全脑缺血模型.免疫组织化学法检测海马区磷脂酰肌醇3-激酶(PI3-K)、蛋白激酶B(PKB/Akt)的表达.原位缺口末端标记法(TUNEL)检测凋亡细胞.结果:与假手术组比较,模型组中PI3-K、Akt表达增高,凋亡神经细胞数量增多;与模型组比较,参芎化瘀胶囊组中PI3-K、Akt表达进一步增多,凋亡神经细胞数量减少,上述变化在高剂量参芎化瘀胶囊组更为明显.结论:参芎化瘀胶囊可抑制全脑缺血大鼠神经细胞凋亡,其机制与PI3-K/Akt信号通路有关.     

依达拉奉对弥漫性脑创伤大鼠的保护作用

Objective To investigate the effects of Edaravone (Ed) on p38mitogen-activated protein kinases/Caspase-3 (p38MAPK/Caspase-3) pathway following the diffuse injury of brain (DIB) in rats, as well as the protective effects of Edaravone on traumatic injury of brain (TIB). Method The TIB models were established by using Marmarou's method in adult male Spraque-Dawlley rats. A total of 250 rats were divided (random number)9nto control group, model group, low-dose Edaravone treatment group and high-dose Edaravone treatment group.The rats were sacrificed separately 1, 6, 24, 48 and 72 hours after DIB and the brain tissues of rats were taken.The morphological changes of neuron in hippocampus region were observed by using Nissl staining. The levels of phosphorylated p38MAPK and Caspase-3 were detected by using immunohistochemistry and Western blot. The learning and memory functions were determined with Morris water maze test from the 3rd to 7th day after injury.Results Compared with control group, some neurons displayed histopathological changes of necrosis and apoptosis in rats of model group. The levels of phosphorylated p38MAPK significantly increased in 1, 6, 24, and 48 hours after injury in rats of model group ( P ＜ 0.05), but there was no statistic significance in increase 72 hours later ( P＞ 0.05). The levels of Caspase-3 significantly increased in 6, 24, 48 and 72 hours after injury in rats of model group ( P ＜ 0.05), but there was no significant difference in increase in one hour after injury (0.59±0.29 vs.0.40±0.17, P ＞0.05).In the Morris water maze test from the 3rd to 6th day, the latency to find the platform significantly prolonged in rats of model group ( P ＜ 0.05), and the numbers of passing the platform by rats decreased on the 7th day (2.28 ± 1.18 vs. 8.20 ± 1.52, P ＜ 0.05). Compared with model group, the levels of phosphorylated p38MAPK in 6, 24 and 48 hours after injury in low-dose Edaravone group were lower (P ＜0.05), but there was no statistical difference in one hour after injury ( P ＞ 0.05). The levels of Caspase-3 in 6,24, 48 and 72 hours after injury in rats of low-dose Edaravone group were lower than those of model group ( P ＜0.05). The latency to find the platform significantly shortened ( P ＜ 0.05) and the numbers of passing the platform by rats increased (4.17 ± 1.15 vs. 2.28 ± 1.18, P ＜ 0.05) in low-dose Edaravone group. The above variables changed more prominently in high-dose Edaravone group. Conclusions Edaravone attenuates p38MAPK pathway activation, lowers the level of Caspase-3 following DIB and protects the rats against the traumatic injury of brain.     

一例胆囊切除术后感染致多脏器功能障碍综合征的护理

多脏器功能障碍综合征(multiple organ dysfunction syndrome,MODS)是指严重创伤、严重感染等原发病发生后,同时或序贯发生两个或两个以上的系统或器官的功能不全或衰竭的临床综合征。我科于2011-02-03收治1例因胆囊切除、T型管引流术后感染导致MODS的患者,经过血液净化、纠正酸中毒、抗生素抗感染等治疗,取得良好效果,现报告如下。1病例资料患者,男,63岁,2011-01-25于外院行胆囊切除、T型管引流术,术后恢复尚可,精神、饮食正常,术后第8天无明显诱因突然出现意识模糊、对外界反应迟钝等症状,此     

表面肌电图在帕金森病患者综合运动训练疗效评估中的应用

目的研究表面肌电图（sEMG）在帕金森病（PD）患者综合运动训练疗效评估中的作用。 方法选取20例符合入选标准的PD患者，给予结合视听觉刺激的运动训练，训练前和训练6周后分别采用统一帕金森病评价量表（UPDRS）、10m步行速度测试进行评价，分析在静止站立和步行时下肢臀大肌、股直肌、胫骨前肌、腓肠肌外侧头四块肌肉的sEMG信号特征，指标选用RMS。 结果与训练前比较，UPDRS量表中日常生活活动（ADL）能力和运动功能部分的评定分数有明显下降；10m步行所用时间减少，步行速度明显增加，差异有统计学意义（P<0.05）；静止站立时，两侧肌肉的均方根振幅（RMS）均有明显降低，差异有统计学意义（P<0.05）；步行时，两侧肌肉的RMS有不同程度增加，重症侧股直肌、腓肠肌外侧头和的轻症侧臀大肌的RMS差异有统计学意义（P<0.05）。Wilcoxon符号秩检验结果显示除步行时的重症侧臀大肌外，其余肌肉RMS差异均有统计学意义（P<0.05）。 结论结合视听觉刺激的运动训练可改善ADL、运动及步行功能，sEMG能够定量反映PD患者静止性震颤、肌强直程度和肌肉功能水平，为进一步制订个体化训练方案提供依据。