淋病奈瑟菌孔蛋白PIA真核表达系统的构建及序列分析

目的:构建淋病奈瑟菌孔蛋白PIA(Porin IA)基因的真核表达系统,为淋病DNA疫苗的研究提供材料。方法:PCR技术扩增淋病奈瑟菌捷克标准株的PIA基因片段,将其定向插入真核表达载体pC I-neo多克隆位点中,构建重组表达载体。并用酶切分析、PCR扩增及序列测定方法,对重组载体进行鉴定。结果:淋病奈瑟菌PIA基因被正确克隆到真核表达载体pC I-neo中,测序结果与Genebank中AF090819等菌株序列有99.99%同源性。结论:真核表达系统(pC I-PIA)构建成功。     

高原高寒地区卫勤保障实践与思考

为提高卫勤分队应对多种安全威胁、完成多样化军事任务能力,我部抽组专家医疗队,于近期伴随某部赴某地执行卫勤保障任务。在海拔4 500~4 700m对部队急性高原病防治知识进行技术培训和健康教育;开展高原病防治、医疗紧急救护;采集高原病人体机能有关数据,进行高原病药物优化研究等。在执行任务过程中,我们对一些问题进行了认真研究,特别是针对高原高寒地区卫勤保障工作进行了细致思考。     

中职学生手机依赖现状及影响因素分析

正随着科技的飞速发展,手机已经成为集娱乐、通讯、休闲于一体的生活必备物品~([1])。手机依赖是指过度沉迷于以手机为媒介的各种活动,对手机使用产生强烈、持续的渴求感与依赖感,并导致个体出现明显的社会、心理功能受损的行为~([2])。中职学生比普通高中学生的自由时间多、学习任务轻,所以手机的使用更普遍,手机依赖问题更需引起高度的关注和重视~([3])。本研究拟通过调查中职学生手机依赖现状和     

淋病奈瑟菌感染动物模型的建立及探讨

目的尝试建立淋球菌感染动物模型,为研究淋球菌致病机制提供良好的动物模型。方法采用野生株淋球菌FA1090接种雌二醇预处理的雌性BALB-c小鼠,小鼠生殖道拭子接种WGC选择培养基培养分离淋球菌,取小鼠阴道分泌物涂片Gram染色观察有无淋球菌,并用瑞氏染色观察小鼠阴道分泌物中多形核白细胞(polymorphonuclearleukocytes,PMN)的数目。结果受试小鼠生殖道中淋球菌平均存活了8d(2×106CFU接种量),阴道分泌物涂片Gram染色可见淋球菌粘附在上皮细胞上,88%的受试小鼠发生了炎症。结论成功建立了淋球菌感染的动物模型,为研究淋球菌的致病机制、毒力及抗菌药物的研究奠定了基础。     

锁骨钩钢板配合中药治疗肩锁关节脱位

目的:观察锁骨钩钢板配合中药治疗肩锁关节脱位的临床疗效.方法:将84例肩锁关节脱位患者随机分为治疗组和对照组各42例,治疗组采用锁骨钩钢板内固定配合中药分3期辨证治疗,对照组采用克氏针张力带钢丝内固定.结果:所有患者术后随访8个月-4年,平均为2.2年.根据karlsson的疗效标准,治疗组的优良率为97.6%,对照组为78.6%,两组比较,差异有统计学意义(P＜0.05).结论:锁骨钩钢板配合中药是治疗肩锁关节脱位的一种好方法.     

经皮腰椎间盘后外侧入路"三角工作区"的薄层断面解剖和三维重建

目的 探明经皮腰椎间盘后外侧入路"三角工作区"的连续薄层断面的形态和三维结构.寻求解决"工作三角"的确切定义. 方法 选取2例中国数字化可视人体数据,对经皮腰椎问盘后外侧入路的连续断面形态进行解剖学观测并进行三维重建. 结果 获得经皮腰椎间盘后外侧入路的连续薄层断面形态学特征、相关测量参数和三维结构."三角工作区"的面积为(23.70±8.73)mm2,腰椎间盘穿刺虽是比较安全的区域,但有腰动脉脊支、椎间静脉和椎内静脉通过. 结论 经皮腰椎问盘后外侧入路"工作三角"的连续薄层断面数据集和三维重建可良好显示其形态和三维结构.初步明确了"工作三角"的定义,进一步证实腰动脉脊支、椎间静脉和椎内静脉是穿刺出血的解剖学基础.     

石骨症1例

1 病例资料 患者,女,38岁,摔伤致左大腿疼痛,肿胀,活动受限3月余.曾在外院行左下肢骨牵引50余天,未见明显骨痂生长,转入我科行切开复位钢板螺钉内固定.     

月经期断指再植围手术期的心理护理

通过对2008年6月～2012年3月本院收治的35例(42指)月经期手指离断性损伤行急诊清创断指再植术的系统护理资料进行回顾性分析,认为充分的术前评估、心理护理及术前准备,术中患者密切配合,术后严密的病情观察、积极对症处理及精心护理,是确保月经期手指离断性损伤患者手术成功的重要环节.     

Mitofusin2 decreases intracellular cholesterol of oxidized LDL-induced foam cells from rat vascular smooth muscle cells

Mitofusin2 (Mfn2) plays a pivotal role in the proliferation and apoptosis of vascular smooth muscle cells (VSMCs). The purpose of this study was to investigate the effects of Mfn2 on the trafficking of intracellular cholesterol in the foam cells derived from rat VSMCs (rVSMCs) and also to investigate the effects of Mfn2 on the expression of adenosine triphosphate-binding cassette subfamily A member 1 (ABCA1), adenosine triphosphate-binding cassette subfamily G member 1 (ABCG1) and peroxisome proliferator-activated receptor gamma (PPARγ). The rVSMCs were co-cultured with oxidized low density lipoprotein (LDL, 80 μg/mL) to produce foam cells and cholesterol accumulation in cells. Before oxidized LDL treatment, different titers (20, 40 and 60 pfu/cell) of recombinant adenovirus containing Mfn2 gene (Adv-Mfn2) were added into the culture medium for 24 h to transfect the Mfn2 gene into the rVSMCs. Then the cells were harvested for analyses. The protein expression of Mfn2 was significantly higher in Adv-Mfn2-transfected group than in untransfected group (P<0.05), and the expression levels significantly increased when the titer of Adv-Mfn2 increased (P<0.05). At 24 or 48 h after oxidized LDL treatment, rVSMCs became irregular and their nuclei became larger, and their plasma abounded with red lipid droplets. However, the number of red lipid droplets was significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group. At 48 h after oxidized LDL treatment, the intracellular cholesterol in rVSMCs was significantly increased (P<0.05), but it was significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05), and it also significantly decreased when the titer of Adv-Mfn2 increased (P<0.05). The mRNA and protein expression levels of ABCA1 and ABCG1 were significantly increased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05). Though the mRNA and protein expression levels of PPARγ was not significantly increased (P>0.05), the phosporylation levels of PPARγ were significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group (P<0.05). These results suggest that the transfection of Adv-Mfn2 can significantly reduce intracellular cholesterol in oxidized LDL-induced rVSMCs possibly by decreasing PPARγ phosporylation and then increasing protein expression levels of ABCA1 and ABCG1, which may be helpful to suppress the formation of foam cells.     

改良包扎术在股动脉假性动脉瘤抢救中的应用价值

目的探讨改良包扎术在毒品注射所致股动脉假性动脉瘤抢救中的应用价值。方法2018年1月至2019年3月对我院收治的15例吸毒所致破裂性股动脉瘤患者先采用改良包扎术进行抢救,观察患肢包扎前后皮温、皮肤颜色、肿胀情况,观察其对动脉供血及静脉回流的影响,记录包扎后至术前重复包扎次数、渗血次数、血红蛋白变化来判断其稳定性。结果所有患者包扎后都能顺利完成术前准备,其中8例行股动脉假性动脉瘤切除+髂股动脉人工血管旁路术,1例因瘤体巨大且局部感染严重行股动脉假性动脉瘤切除+髂股动脉结扎术,4例行覆膜支架腔内隔绝手术,2例行股动脉修补术;无改急诊手术患者;无肢体坏死患者。无改急诊手术患者,亦无肢体坏死患者。结论在毒品注射所致股动脉假性动脉瘤患者的抢救中,改良包扎术是一种高效、安全的方法,且并发症少,可作为抢救治疗的首选包扎方法。