人蛋白酶体α7亚基在原发性肝细胞肝癌中的表达及临床意义

目的:探讨人蛋白酶体α7亚基(PSMA7)与原发性肝细胞肝癌(HCC)发生发展的关系。方法:采用免疫组织化学法检测254例HCC中PSMA7蛋白的表达。结果:在癌组织细胞质中,发现不同临床分期和病理分级之间,PSMA7的表达均无明显差异(P>0.05),但不同肝硬化程度间具有显著性差异(P<0.01),以早期肝硬化患者肝细胞表达量最高。在癌组织细胞核中,不同临床分期之间PSMA7的表达无明显差异(P>0.05),但不同病理分级之间具有显著性差异(P<0.05),同时与肝硬化的程度有一定关系(P<0.05),亦以早期肝硬化患者肝细胞表达量最高。另外,PSMA7的表达在癌组织和癌旁组织的细胞质中无明显差异(P>0.05),而在细胞核中具有显著性差异,癌组织高于癌旁组织(P<0.01)。淋巴结转移组细胞核中的PSMA7表达高于无淋巴结转移组(P<0.05)。结论:PSMA7在HCC细胞核中表达增高,与病理分期有关。此外,PSMA7的表达与肝硬化程度和淋巴结转移有关。     

C3基因单核苷酸多态性与成人哮喘易感性的相关性研究

目的 探讨C23基因的单核苷酸多态性与华南汉族成人支气管哮喘发病的相关性.方法 应用病例对照研究,收集2006年2月至2010年2年南方医院和广东医学院附属医院确诊的汉族成人哮喘患者484例为研究对象,同期健康查体者553名为对照,应用MassARRAY-IPLEX技术和基质辅助激光解吸电离飞行时间质谱平台(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF-MS)对C3基因的rs10402876和rs366510位点进行基因分型.结果 在华南汉族人群中rs10402876检测到GG、GC和CC 3种基因型,rs366510检测到GG、GT和TT 3种基因型,分型成功率为98.94%.与对照组相比,哮喘组rs10402876的基因型分布(χ2=0.346,P=0.841)和等位基因分布(χ2=0.101,P=0.751)差异均无统计学意义;而rs366510的基因型分布(χ2=9.759,P=0.008;Bonferroni校正法,P=0.016)和等位基因分布(χ2=5.294,P=0.021;Bonferroni校正法,P=0.042)差异均有统计学意义.与GG+GT基因型相比,rs366510的TT基因型显著增加哮喘发生的危险性(OR=1.471,95%CI:1.125～1.923).结论 C3基因rs366510位点单苷酸多态性可能与华南汉族成人哮喘易感性有关.

Abstract：

Objective To investigate the association between single nucleotide polymorphisms (SNPs) of the complement component 3 gene (C3) and adult asthma of Hans in southern China. Methods A casecontrol study was performed. Four hundred and eighty-four adult asthma patients diagnosed in Nanfang Hospital and Affiliated Hospital of Guangdong Medical College, and 553 healthy subjects were collected from 2006 to 2010 for the study. MassARRAY-IPLEX and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) techniques was used to determine the genotypes of the rs10402876 and rs366510 loci of C3 gene. Results Genotypes GG, GT and TT in the rs366510 locus, and genotypes GG, GT and TT in the rs10402876 locus were detected. A total of 98. 94 percent of samples were genotyped. There were no significant differences in genotype frequencies (χ2 =0. 346, P=0. 841 ) and allele frequencies (χ2 =0. 101,P=0. 751) of rs10402876 between the two groups. However, genotype and allele frequencies of the rs366510 locus were significantly different (χ2 = 9.759, P=0. 008, Bonferroni correction,P= 0. 016; χ2 = 5. 294, P= 0. 021, Bonferroni correction, P = 0. 042, respectively). Compared with genotypes GG+GT, genotype TT of rs366510 significantly increased the risk of asthma, with the odds ratio of 1. 471 (95 % confidence interval 1. 125-1. 923). Conclusion These results suggest that C3 gene could be associated with adult asthma of Han population in southern China.     

急性冠状动脉综合征患者血清晚期氧化蛋白产物水平的测定意义

目的 探讨血清晚期氧化蛋白产物（AOPP）水平在急性冠状动脉综合征（ACS）中的意义及对预后的影响.方法 选择ACS患者共55例,其中不稳定型心绞痛（UAP）29例,急性心肌梗死（AMI）26例;正常对照组30例;采用分光光度计法检测所有入选者的血清AOPP浓度,并记录4周内所有患者的病情转归情况.结果 2组ACS患者AOPP水平均明显高于正常对照组（均P＜0.01）,AMI组AOPP水平高于UAP组（P＜0.05）;病情转归及预后差者AOPP水平相对高（P＜0.01）.结论 AOPP与ACS的发生有关,并且与病情的严重程度呈正相关,对病情转归有一定预测价值.     

年轻人急性心肌梗死的特点

急性心肌梗死 (AMI)发病率近年有明显增高趋势 ,且随年龄增加而增高。对老年人 ,AMI无论是基础还是临床都有较多研究 ,一般都能及时诊治 ,但对于年轻人AMI(<4 0岁 )国内研究较少 ,且往往与非心脏疾患相混淆 ,以至延误病情。作者就年轻人AMI特点作一综述 ,以利于其诊治。1　发病情况据日本统计 ,年轻人AMI并不罕见 ,其发生率占总AMI的 1 2 %～ 4 5 % ,在Miyamoto[1] 统计的AMI 2 879例 ,年轻男性 37例 (36± 4岁 ,2 3～ 39岁 )。意大利Imazio[2 ] 在大样本的流行病学调查中报道 ,GISSI 2和GISSI 3两组中年轻人AMI的发生率分…     

Sex Hormones Affect Aging Process by Influencing Lipid Profiles, Cellular Immunological Function and Lipid Peroxides and Oxidation System

Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA, SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone ( FSH)、luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life; Both testosterone (T) and free testosterone (FT) all decreased greatly with aging, but 17β - estradiol( 17β - E_2) was reverse ; E_2 was negatively correlated with T and E_2/T increased with aging. The level of serum total cholesterol (TC) increased with aging, but triglycerides (TG) remain unchanged; compared with young group, high - d     

无创连续气道正压通气辅助治疗妊娠高血压并发心力衰竭效果观察

目的：观察无创连续气道正压通气辅助治疗妊娠高血压并发心力衰竭的临床效果。方法选取80例妊娠期高血压并发心力衰竭患者为研究对象,在解痉、降压、利尿、强心等常规治疗基础上给予辅助无创连续气道正压通气,观察治疗前后3h 心率、呼吸、氧分压（PaO2）与二氧化碳分压（PaCO2）改善情况及效果。结果患者治疗后心率、呼吸较治疗前下降,PaO2升高（ P ＜0．01）,治疗后PaCO2较治疗前无明显变化（ P ＞0．05）,治疗总有效率为97．50％。结论妊娠期高血压并发心力衰竭患者在常规治疗基础上给予持续气道正压通气辅助治疗有利于及时改善患者呼吸困难及发绀等症状,有效纠正低氧血症,疗效确切。     

参芪扶正注射液联合无创机械通气治疗心力衰竭的疗效分析

目的：对参芪扶正注射液联合无创机械通气治疗心力衰竭的临床疗效进行分析探讨。方法：选择2011年1月至2014年1月我院收治的心力衰竭患者120例为研究对象,将患者随机分为对照组和治疗组,各60例,对照组给予常规吸氧及药物治疗;治疗组在对照组的基础上给予静脉滴注参芪扶正注射液及无创机械通气。对比分析两组患者在治疗前后临床症状改善情况。结论：经过相应治疗后,与对照组比较,治疗组患者在心脏功能改善方面显著优于对照组,治疗组患者临床总有效率为88．34％显著高于对照组的68．33％, P ＜0．05,差异具有统计学意义。结果：参芪扶正注射液联合无创机械通气治疗心力衰竭能够很好的改善患者临床症状,取得良好的临床效果。     

伴葡萄糖耐量低减的老年非ST段抬高急性冠状动脉综合征患者的临床特点

近年来，作为糖尿病(DM)前期的检查指标葡萄糖耐量低减(IGT)日益引起重视，我们于2002年4月至2002年11月对老年伴IGT和DM的非ST段抬高的急性冠状动脉(冠脉)综合征(ACS)患者的临床特点进行观察，旨在了解IGT对心血管疾病的影响。     

Effects of Estrogen Level on the Function of Vascular Endothelial Cells and Expression of Vascular Cell Adhesion Molecule-1(?)

Objectives To ob-serve the effect of different estrogen levels on the secretory function of vascular endothelial cells of female rats, and study the effect of modulation of estrogen level on the expression of vascular cell adhesion molecule - 1 and the concentration of estrogen receptor in vascular endothelial cells. Methods Radioim-munology was used to measure the serum concentration of endothelin and PGI2, and copper - cadmium reduction was employed to measure the serum content of nitrogen monoxide. Radioligand binding and flowcy-tometry were used to measure the expression of estrogen receptor and vascular cell adhesion molecule (VCAM - 1) of vascular endothelial cells respectively. Results 1. The serum concentration of nitric oxide and PGI2 decreased when the ovaries of female rats were removed. In ovariectomized rats, given estrogen, the concentration rose ( P < 0. 05), but the plasma concentration of endothelin was adverse to it. 2. The concentration of estrogen receptor of vascular endothelial cel