心脏永久起搏手术的前后护理

护理部主任:根据护理管理目标计划,第一季度在心内科进行一次护理查房,现全院护士及心内科的全体护理人员已到齐,查房开始。心内科护士长:由心脏疾病引起的晕厥是我科常见病,但安装心脏起搏器则是我科开展的新项目,虽然在这之前已成功的为6例患者安装了心脏起搏器,但     

中西医结合治疗口腔粘膜扁平苔癣60例临床观察

中西医结合治疗口腔粘膜扁平苔癣６０例临床观察河北省农科院承德桑蚕研究所医务室高智慧河北省人民医院中医科许秀兰关键词扁平苔癣；口腔粘膜；疗效；中西医结合口腔粘膜扁平苔癣是一种较常见的皮肤和粘膜的慢性炎性疾病。它可以同时发生于皮肤和粘膜，也可以单独发生于...     

In vitro effect of p21WAF-1/CIP1 gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system

Objective: To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21WAF-1/CIP1 gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-viral vector GE7 gene delivery system was constructed. The malignant human glioma cell line U251MG was transfected in vitro with β-galactosidase gene(reporter gene) and p21WAF-1/CIP1 gene (therapeutic gene) using the GE7 system. By means of X-gal staining, MTS and FACS, the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21WAF-1/CIP1 gene in transfected U251MG cell was examined by immunohistochemistry staining. Results: The highest transfer rate of exogenous gene was 70%. After transfection with p21WAF-1/CIP1 gene, the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently. FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the ability to transfer exogenous gene to targeted cells efficiently, and expression of p21WAF-1/CIP1 gene can induce apoptosis of glioma cell and inhibit its growth.     

pLTKcSN／VPC及GCV系统的旁观者效应观察

目的：探讨单纯疱疹病毒Ｉ型胸苷激酶基因（ＴＫ）逆转录病毒载体生产细菌（ｐＬＴＫｃＳＮ／ＶＰＣ）和更昔洛韦（ＧＣＶ）系统杀伤恶性胶质瘤细胞过程中的旁观者效应。方法：采用大鼠胶质瘤细胞Ｃ６、人恶性胶质瘤Ｕ８７ＭＧ和它们的转染ＴＫ阳性细胞Ｃ６ＴＫ，Ｕ８７ＴＫ，将Ｃ６与Ｃ６ＴＫ，Ｕ８７ＭＧ和Ｕ８７ＴＫ按比例（ＴＫ阳性细胞占细胞总体的０－１００％，１０％梯度）分别混合培养于９６孔板中，每种混合比例设６孔。     

醋酸联合靛胭脂染色对胃黏膜病变诊断的临床价值

目的：探讨内镜下醋酸联合靛胭脂染色对胃黏膜病变的临床诊断价值。方法选择高危患者356例,常规胃镜检查后行醋酸联合靛胭脂染色,分别观察并记录染色前后可疑病变的形态及色泽变化并进行活检。结果①染色检查的低级别上皮内瘤变、高级别上皮内瘤变、早期胃癌检出率均高于常规检查,正常及炎症检出率低于常规检查（P 〈0.05）。②常规检查检出的隆起型病灶占染色检查检出的44.9%,检出正确的平坦型病灶占染色检查检出的55.6%,检出凹陷型病灶占染色检查检出的63.0%;合计检出正确病灶率占染色检查检出的48.7%。结论内镜下醋酸联合靛胭脂染色可显著提高早期胃癌及高级别上皮内瘤变检出率。     

人肺癌相关抗原的提取、抗血清制备及初步鉴定

原发性肺癌(以下简称肺癌)是严重危害人民健康的常见恶性肿瘤,目前尚缺乏理想的诊断和治疗方法。沿用的X线和细胞学方法在早期诊断和鉴别诊断方面甚感不足,肺癌相关抗原的研究将有助于提高诊断率。自1968年Yachi开始对肺癌抗原进行研究以后,有些研究工作者用微量细胞毒、迟缓型超敏试验、白细胞移动抑制试验(LMI)、白细胞粘附抑制试验(LAI)等细胞免疫方法,证实肺癌组织于3M KCl提取液中存在     

正常肝细胞核糖核酸对肝癌细胞的生物学作用

肿瘤细胞是一种分化与生长异常的细胞,细胞癌变一向被认为是一种不可逆的、去分化的过程。能否使癌细胞再分化,对于癌生长的控制与治疗将有重要的意义。我们从正常小鼠肝制备了两种核糖核酸,即水层 RNA 与酚层RNA。应用水层 RNA(每毫升8毫克)处理小鼠腹水型肝癌细胞后,癌细胞失去了生长成