辐照氟银猪皮的实验研究

报道辐照氟银猪皮的制备、实验检测(弹性、创面粘附力和透水性测定,含银量测定)组织学检查细菌检测及抑菌试验研究,结果表明:辐照氟银猪皮除延伸度低于未辐照猪皮外,其撕裂强度、创面粘合力和透水性均与未辐照猪皮相似。组织学检查示:辐照氟银猪皮组织结构完整,表皮细胞部分空泡形成。组织细菌检测证明其无菌生长,达到无菌要求。抑菌试验证明对金黄色葡萄球菌、绿脓杆菌、大肠杆菌、变形杆菌、克雷伯氏菌均有极强的抑菌作用。     

CD3AK细胞对烧伤患者T淋巴细胞亚群的影响

观察 CD3AK细胞用于严重烧伤患者后对 T淋巴细胞亚群的影响。观察对象为 CD3AK细胞治疗的 2 0例大面积烧伤患者。结果 :经 CD3AK细胞治疗后烧伤患者 T细胞亚群的结构有了明显的改善 ,CD4细胞比例明显升高 ,CD3细胞比例下降 ,CD4/ CD8比值升高。结论 :CD3AK细胞对烧伤患者 T细胞亚群的结构有明显的改善 ,从而提高烧伤患者的免疫力。     

烧伤免疫的研究现状

烧伤后机体免疫功能发生严重紊乱 ,主要表现为免疫功能低下 ,已被大量的研究证实。免疫功能低下使烧伤患者极易发生感染 ,感染是烧伤患者主要死亡原因之一。因此 ,近年来烧伤后机体免疫功能的问题 ,已被越来越多的学者所重视。做了大量的研究工作 ,取得了一定的成绩 ,使人们对     

加味四君子汤对严重烫伤兔肠黏膜及mTOR信号通路相关基因表达的影响

目的 探讨加味四君子汤对严重烫伤兔肠黏膜及哺乳动物雷帕霉素靶蛋白(mTOR)信号通路相关基因表达的影响.方法 成功建立严重烫伤模型的72只日本大耳兔分为烫伤模型对照组(A组,18只)和加味四君子汤处理(B组,54只)两组.B组又分为三亚组,分别每日用加味四君子汤0.2 g/mL(B1组)、1.0 g/mL(B2组)或5.0 g/mL(B3组)灌胃,连续7 d.A组予以等量生理盐水灌胃作为对照.另取18只大耳兔为空白对照(C组),自由饮食、饮水.分别于第1、3、7天各组处死6只大耳兔,采用ELISA法检测各组回肠黏膜组织中TNF-α、IL-10和IL-1β水平.选取治疗效果最佳的第7天B2组、第7天A组和C组大耳兔回肠黏膜组织,采用荧光定量PCR和Western blot分别检测mTOR信号通路相关基因mRNA和蛋白表达.结果 与C组比较,A、B1、B2和B3组T N F-α和IL-1β水平升高,而IL-10水平降低(P<0.05).与A组比较,B1、B2和B3组T N F-α和IL-1β水平下降,而IL-10水平升高(P<0.05),其中B2组T N F-α和IL-1β水平下降以及IL-10水平升高更明显(P<0.05).与C组比较,A组磷脂酰乙醇胺结合蛋白1(PEBP1)和真核起始因子4F(EIF4G1)mRNA和蛋白表达升高,而酪蛋白激酶Ⅱ亚基(CSNK2A1)、真核生物翻译起始因子3亚基I(EIF3I)、蛋白磷酸酶2催化亚基(PPP2CA)和丝裂原激活蛋白激酶1(MAP2K1)mRNA和蛋白表达降低(P<0.05).与A组比较,B2组PEBP1、CSNK2A1和EIF4G1 mRNA和蛋白表达降低,而EIF3I、PPP2CA和MAP2K1 mRNA和蛋白表达升高(P<0.05).结论 加味四君子汤可通过调控mTOR信号通路相关基因表达,从而减轻严重烫伤兔回肠黏膜炎症反应.     

强化精氨酸肠内营养对烧伤患者营养状况和细胞免疫的影响

Objective To investigate the effects of arginine enriched enteral nutrition (EN) on nu-tritional status and cellular immunity of severely burned patients. Methods Randomized, single blind, parallel and positive control investigation was employed in the study. Thirty severely burned patients were di-vided into enteral immune nutrition (EIN) group and EN group. Sixteen patients in EIN group received en-teral nutrition enriched with arginine, while the other 14 patients in EN group received standard enteral nu-trition. Nutritional support was continued for 14 days. Gastrointestinal reaction of patients in 2 groups was observed. Fasting venous blood was drawn from patients of both groups before receiving nutrition treatment and on the morning of 7th, 14th day of treatment. Level of serum protein, hepatic function parameters, renal function parameters, fasting-blood glucose, and subpopulations of T lymphocytes in peripheral blood were determined. Results (1) Incidence of gastrointestinal side effect in EIN group (25.0%) was close to that of EN group (21.4% , P>0.05). (2) Compared with pre-treatment days, levels of prealbumin and transferrin in serum of patients in 2 groups on 7th and 14th post-treatment days were significantly increased (P<0.05 or P<0.01), but there was no significant difference between 2 groups. The level of total serum protein on 14th day of treatment of patients was significantly increased in both groups, and that of EIN group (66±7 g/L)was significantly higher compared with that in EN group (64 ± 11 g/L, P<0.05). The level of serum albumin (29±5, 32±5 g/L, respectively) of patients in EIN group on 7th and 14th day of treat-ment were significantly higher than that (26±4 g/L, P <0.05) in pre-treatment days, however there was no significant difference in EN group. (3) There was no significant difference in respect of hepatic function, renal function, and fasting-blood glucose between pre-treatment and post-treatment periods in both groups (P>0.05). (4) The ratio of CD4+ , CD8+ on 14th day of treatment in EIN group was close to that of pre-treatment level. In EN group, cell percentage of CD4+ significantly decreased, while that of CD8+ significantly increased (P<0.05), and CD4+ was significantly higher [(56±8) %] in EIN group than that in EN group [(55±12) % , P <0.05]. In both groups, cell percentage of CD3+ was significantly higher than that in pre-treatment days (P<0.05), while there was no obvious change in CD4+/CD8+. Conclusions Arginine enriched enteral nutrition can effectively improve nutritional status and cellular immune function of burn patients.     

烧伤创面细菌学调查分析

败血症是烧伤患者的主要死亡原因之一‘’,’‘,烧伤创面细菌是烧伤败血症细菌的主要来源。因此,及时了解烧伤创面细菌分布及生态学的变化,对指导烧伤创面的处理及烧伤败血症的预防和治疗有其重要的意义。本文』Q．结了我科1986～1997年创面培养菌4649株,并分1986～1990年     

中药膳食对烫伤大鼠肠源性感染防治作用

目的 了解中药膳食对严重烫伤大鼠肠源性感染防治效果,探索临床防治烧伤后肠源性感染的新方法。方法 健康Wistar大鼠100只,随机分为药膳组、肉汤组、常规组(n=30)和对照组(n=10);药膳、肉汤、常规组于伤后第1、3、7 d各取10只,无菌条件下取材检测肠粘膜病理形态学、血浆内毒素含量、细菌移位率、盲肠膜菌群、肠黏液中分泌型免疫球蛋白A(sIgA)含量。结果 与对照组比较,伤后药膳、肉汤、常规组细菌移位率、内毒素水平升高、酵母菌及大肠杆菌数量明显增多,肠道slgA水平由(59.31±8.66)μg/mL降至(38.12±4.49)μg/mL、双歧杆菌数量由(7.6±0.6)log CFU/g降至(6.3±0.5)log CFU/g(P<0.05);与肉汤、常规组比较,药膳组细菌移位率、内毒素水平降低、酵母菌及大肠杆菌明显减少,肠道slgA水平升高至(53.06±5.23)μg/mL,双歧杆菌数量明显增多(P<0.05)。结论 严重烫伤破坏了肠道粘膜屏障功能,引起细菌、内毒素移位,应用中药膳食干预,能有效预防肠源性感染。     

强化精氨酸肠内营养对烧伤患者营养状况和细胞免疫的影响

Objective To investigate the effects of arginine enriched enteral nutrition (EN) on nu-tritional status and cellular immunity of severely burned patients. Methods Randomized, single blind, parallel and positive control investigation was employed in the study. Thirty severely burned patients were di-vided into enteral immune nutrition (EIN) group and EN group. Sixteen patients in EIN group received en-teral nutrition enriched with arginine, while the other 14 patients in EN group received standard enteral nu-trition. Nutritional support was continued for 14 days. Gastrointestinal reaction of patients in 2 groups was observed. Fasting venous blood was drawn from patients of both groups before receiving nutrition treatment and on the morning of 7th, 14th day of treatment. Level of serum protein, hepatic function parameters, renal function parameters, fasting-blood glucose, and subpopulations of T lymphocytes in peripheral blood were determined. Results (1) Incidence of gastrointestinal side effect in EIN group (25.0%) was close to that of EN group (21.4% , P>0.05). (2) Compared with pre-treatment days, levels of prealbumin and transferrin in serum of patients in 2 groups on 7th and 14th post-treatment days were significantly increased (P<0.05 or P<0.01), but there was no significant difference between 2 groups. The level of total serum protein on 14th day of treatment of patients was significantly increased in both groups, and that of EIN group (66±7 g/L)was significantly higher compared with that in EN group (64 ± 11 g/L, P<0.05). The level of serum albumin (29±5, 32±5 g/L, respectively) of patients in EIN group on 7th and 14th day of treat-ment were significantly higher than that (26±4 g/L, P <0.05) in pre-treatment days, however there was no significant difference in EN group. (3) There was no significant difference in respect of hepatic function, renal function, and fasting-blood glucose between pre-treatment and post-treatment periods in both groups (P>0.05). (4) The ratio of CD4+ , CD8+ on 14th day of treatment in EIN group was close to that of pre-treatment level. In EN group, cell percentage of CD4+ significantly decreased, while that of CD8+ significantly increased (P<0.05), and CD4+ was significantly higher [(56±8) %] in EIN group than that in EN group [(55±12) % , P <0.05]. In both groups, cell percentage of CD3+ was significantly higher than that in pre-treatment days (P<0.05), while there was no obvious change in CD4+/CD8+. Conclusions Arginine enriched enteral nutrition can effectively improve nutritional status and cellular immune function of burn patients.     

强化精氨酸肠内营养对烧伤患者营养状况和细胞免疫的影响

Objective To investigate the effects of arginine enriched enteral nutrition (EN) on nu-tritional status and cellular immunity of severely burned patients. Methods Randomized, single blind, parallel and positive control investigation was employed in the study. Thirty severely burned patients were di-vided into enteral immune nutrition (EIN) group and EN group. Sixteen patients in EIN group received en-teral nutrition enriched with arginine, while the other 14 patients in EN group received standard enteral nu-trition. Nutritional support was continued for 14 days. Gastrointestinal reaction of patients in 2 groups was observed. Fasting venous blood was drawn from patients of both groups before receiving nutrition treatment and on the morning of 7th, 14th day of treatment. Level of serum protein, hepatic function parameters, renal function parameters, fasting-blood glucose, and subpopulations of T lymphocytes in peripheral blood were determined. Results (1) Incidence of gastrointestinal side effect in EIN group (25.0%) was close to that of EN group (21.4% , P>0.05). (2) Compared with pre-treatment days, levels of prealbumin and transferrin in serum of patients in 2 groups on 7th and 14th post-treatment days were significantly increased (P<0.05 or P<0.01), but there was no significant difference between 2 groups. The level of total serum protein on 14th day of treatment of patients was significantly increased in both groups, and that of EIN group (66±7 g/L)was significantly higher compared with that in EN group (64 ± 11 g/L, P<0.05). The level of serum albumin (29±5, 32±5 g/L, respectively) of patients in EIN group on 7th and 14th day of treat-ment were significantly higher than that (26±4 g/L, P <0.05) in pre-treatment days, however there was no significant difference in EN group. (3) There was no significant difference in respect of hepatic function, renal function, and fasting-blood glucose between pre-treatment and post-treatment periods in both groups (P>0.05). (4) The ratio of CD4+ , CD8+ on 14th day of treatment in EIN group was close to that of pre-treatment level. In EN group, cell percentage of CD4+ significantly decreased, while that of CD8+ significantly increased (P<0.05), and CD4+ was significantly higher [(56±8) %] in EIN group than that in EN group [(55±12) % , P <0.05]. In both groups, cell percentage of CD3+ was significantly higher than that in pre-treatment days (P<0.05), while there was no obvious change in CD4+/CD8+. Conclusions Arginine enriched enteral nutrition can effectively improve nutritional status and cellular immune function of burn patients.