骨髓基质培养上清作用下脐血造血细胞体外生长与表面标志的关系

目的 探讨骨髓基质细胞培养上清(SN)协细胞因子对人脐血造血细胞体外生长的影响。方法 使用rIL-3，rI-1β，rIL-6，和SCF等细胞因子或SN或者两者联用长期培养人脐血造血细胞，进行细胞增殖和表面标志测定。结果 培养7d细胞已开始增殖，14d达高峰，细胞总数在细胞因子组(1)增加了134．5倍和SN组(3)增加81．5倍，两者联用(2)增加了171．3倍。21d开始下降，(3)组下降最明显，35d时细胞总数为培养前的(3)组26．65倍，(1)组54．15倍．(2)仍组有92．25倍。CD34^ 细胞第7天已升高。14d达高峰。(1)、(3)组和(2)组分别比培养前增加了68．43％、69．77％和82．47％，2ld开始减少。分别比培养前增加56．16％、12．33％和71．72％；CD33^ 细胞7天时均显著增加，14d达高峰，分别比培养前增加了80．67％、82．14％和69．69％，21d时维持在14d水平；CD38 细胞7d时均有显著增加，分别比培养前增加了66．65％、70．42％和73．8％，14～21d均在该水平维持，三组问无显著性差异。结论 骨髓基质细胞培养上清可促进脐血造血细胞的增殖，但比细胞因子作用弱，两者联用时作用明显强于两者分别使用，提示骨髓基质细胞还可产生其它物质刺激造血细胞增殖。     

护士长不良管理方法的浅析及对策

随着医院体制的改革，医疗护理模式的转变，对现代护士长的管理提出了更高的要求。护士长要想适应新的管理模式，只有克服不良的管理方法，才能有效的发挥领导作用，充分调动护士的积极性、开拓性，激发她们的爱岗敬业、无私奉献精神，共同提高护理质量，满足各层次人员的需求。     

拔伸牵引合点压手法治疗腰椎间盘突出症208例

腰椎间盘突出症是一种临床常见病、多发病.笔者近4年来采用拔伸牵引结合点压手法治疗腰椎间盘突出症208例,取得了较满意疗效,现介绍如下.     

Recent advances of perinatal medicine in China.

Perinatal medicine, known also as maternal and fetal medicine, is a new branch of medicine with only a history of 30 years. China has started the perinatal medicine since 1979. The 1st Session of Pan-China Meeting of Perinatal Medicine was held in Shanghai in 1981. In 1983, experts from 8 European countries discussed the development and popularization of perinatal medicine and surveillance technology with the Chinese scholars in Euro-China Consultation on Perinatal Surveillance in Beijing, as jointly sponsored by the Ministry of Health of Chinese Government and WHO-EURO. In the following years, 3 training centers were installed in Shanghai, Wuhan and Harbin by the United Nations Fund for Population Activities (UNFPA) and the Ministry of Health. China. Series of courses on perinatal surveillance technology were given yearly for 7 years to about 1 000 participants from all the leading universities and municipal hospitals in all provinces of China, with subsequent rapid propagation of technology all over Ch     

亚硝酸盐中毒84例抢救护理体会

亚硝酸盐是一种化学工业试剂，也是一种氧化剂，具有防腐的作用，有些饭店常用它作为熟肉的添加剂。剂量过太，或误用常常会造成食物中毒。我科从1992年以来收治亚酸盐中毒患72倒，现将抢救护理体会总结如下。