Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

国产法莫替丁治疗消化性溃疡多中心双盲及开放的临床研究

我们用青岛制药厂生产的新H_2受体阻滞剂法莫替丁在北京、沈阳、大连、锦州和青岛等七家医院进行了治疗消化性溃疡的多中心临床研究。300例患者服用法莫替丁20mg一天二次共6周,并用甲氰味呱400mg一天一次共6周进行对照(179)。分双盲摸拟和开放两组进行。结果表明法莫替丁在治疗消化溃疡方面疗效与甲氰味呱相似。双盲组中愈合率分别为89.6％与80％(P>0.05),总有效率分别为95.8％与91％(P>0.05)。开放组中法莫替丁愈合率为86.9％,甲氰咪呱组为65％,两组比较P值<0.05。两组的总有效率为96.8％与92.4％(P>0.05)。结果同时表明法莫替丁对十二指肠溃疡和胃溃疡的疗效相似(愈合率为87.5％与86.8％,P>0.05)。未发现法莫替丁组有明显副作用,我们认为,法莫替丁是一种新的、强效的、作用时长的H_2受体阻滞剂,病人易耐受,副作用小,且无抗雄性激素的副作用。     

Celsior液与UW液对无心跳大鼠供肝保存效果的比较

Objective To compare the effects of Celsior (CS) solution vs University of Wisconsin (UW) solution on preservation of non-heart- beating donor (NHBD) livers in rats. Methods The healthy male SD rats were taken as donors and recipients. The thoracic descending aorta and supradiaphragmatic inferior vena cava were cross-clamped for 10 min to induce ischemia as a NHBD model. After flushed with the cold CS or UW solution, NHBD livers were stored at 4℃ for 8 h or 16 h. Then they were transplanted using the two-cuff technique with arterial reconstruction. Accordingly, the recipients were divided into four groups: CS 8 h, UW 8 h, CS 16 h and UW 16 h groups. The bile discharge, liver enzymes (ALT and AST), ET-1, IL-1 and TNF-alpha levels in serum at 1, 3, and 6 h after reperfusion were measured. Histopathological examination was carried out by HE staining, and the 7-day survival rate was observed. Results As compared with a homogenous appearance in the NHBD livers perfused with CS solution, the NHBD livers perfused with UW solution showed a heterogenous appearance, with more or less mottling. In the CS 8 h group there were more bile discharge at 1 h, lower ALT and AST levels at 1, 3 and 6 h, and lower serum ET-1, IL-1 and TNF-alpha ? levels at 1 and 3 h after reperfusion than the UW 8 h group (P<0.05). Histopathological examination showed a well-preserved hepatic architecture, milder portal congestion and inflammatory infiltration in CS 8 h group as compared with UW 8 h group. The 7-day survival rate in CS 8 h and UW 8 h groups was 58.3% and 25.0% respectively (P<0.05). There were no significant differences in bile discharge, ALT, AST, ET-1, IL-1 and TNF-alpha ? levels at different time points between CS 16 h and UW 16 h groups (P>0.05), and histopathological examination showed no much difference in the tissue damage between two groups; all recipients died within 3 days (P>0.05). Conclusion CS was more effective than UW in preserving NHBD livers after 8 h of cold storage, which may be due to the better perfusion of CS and inhibition of Kupffer cells, but it had better preserve NHBD livers with CS no more than 16 h.     

建立医院财务计划管理信息系统的探讨

随着我军现代化、正规化建设的发展,对军队医院财务计划管理工作提出了新的要求,为适应卫勤现代化发展的需要,更好地为医院管理服务,提高医院的经济效益.建立医院财务计划管理信息系统时重要手段,能及时准确的掌握财务计划、执行情况,为医院财务管理控制提供依据.     

浅谈抗生素的相互作用与合理伍用

浅谈抗生素的相互作用与合理伍用王兆文，汪金（新疆疏勒县解放军第１２医院８４４２００）随着抗生素的广泛使用，抗生素之间的联合应用以及抗生素与其它药物配伍应用已较常见。现就其相互作用与不合理伍用的后果表现列举如下，此期引起临床医药工作者的注意。一、相互作...     

新疆喀什、克州驻军肺结核情况调查

为了解南疆部队肺结核情况及其相关因素,进而为部队防治该病提供一定的科学依据,我们对驻新疆南部喀什、克州两地州部队部分单位作了抽样调查,报告如下。１材料与方法。１．１调查地区与受检对象：调查对象为驻新疆南部喀什、克州两地州３个县的部分部队。采用整建制连...     

抗痨药物所致肝损害及其防治

<正>联合用药是结核病化疗的基本原则之一,目前常用的抗痨药物虽然疗效较好,但是毒副作用较大,易发生以肝脏损害为主的不良反应,异烟肼、利福平、吡嗪酰胺的联用尤然,由于肝脏损害造成结核病治疗中断又是导致不合理化疗乃至治疗失败的原因之一,也有因为严重肝损而危及患者生命。近年来,由抗痨药物所致肝损及有关其防治的报道逐渐增多,现就这方面的部分国内文献作以简要综述。引文中常用的抗痨药物有:异烟肼     

大剂量丹参硫酸镁注射液退黄效果观察

1992年3月～1994年12月我科对病毒性肝炎高胆红素血症（SB＞160μmol／L）60例中的32例，应用丹参加硫酸镁治疗，效果满意。现报告如下。1病例选择全部病例为住院患者，均符合1990年5月上海第六届全国病毒性肝炎会议修订诊断标准。对高胆红素血症60例随机分组，治疗组32例，男22例，女10例，年龄4～62岁，平均286岁，SB166.0±8μmol／L；对照组28例，男20例，女8例，年龄8～56岁，平均26．6岁，SB161．2±4．6μmol／L。ALT均＞200U。两组病程，年龄，SB，ALT比较，无显著性差异，P＞0．05，有可比性。2治疗方法两组基础护肝药基本…     

肝细胞癌p53蛋白、增殖细胞核抗原与临床关系的研究

目的　探讨在肝细胞癌中突变型 p5 3蛋白与增殖细胞核抗原 (PCNA)的相互关系及其意义。 方法　应用免疫组化ABC法检测肝细胞癌和肝硬化中 p5 3蛋白及增殖细胞核抗原。 结果　 37例肝细胞癌 (HCC)中 p5 3阳性 19例 ,33例肝硬化标本中p5 3阳性 2例 ,两者有显著差异 (P <0 .0 1) ;HCC中PCNA阳性细胞均数显著高于肝硬化组 (P <0 .0 1) ;p5 3阳性者PCNA阳性细胞数高于p5 3阴性者 (P <0 .0 5 )。p5 3与PCNA两者表达存在显著正相关关系 (r=0 .5 936 2 ,P <0 .0 5 ) ,p5 3表达与年龄、性别、组织分化无关 ,PCNA表达分别与组织分化及血AFP成负相关及正相关。结论　p5 3基因突变在由肝硬化转变为癌过程中是频发事件 ,其表达异常与HCC发生密切相关。PCNA与 p5 3的正相关提示p5 3突变型主要存在于异常增生细胞中 ,且PCNA可反映癌细胞分化及增殖程度 ,可为肝硬变与肝癌关系的探讨及临床诊断、治疗、预后提供参考资料。     

肝动脉化疗栓塞联合替雷利珠单抗成功转化不可切除肝细胞癌一例报道及探讨

目的 探讨肝动脉化疗栓塞（TACE）联合免疫检查点抑制剂（ICIs）在转化不可切除肝细胞癌中的治疗价值。方法 对1例初治不可切除肝细胞癌患者实施TACE联合替雷利珠单抗行转化治疗,序贯挽救性切除的案例的临床病理资料进行分析。结果 患者术前常规行血液学、CT及磁共振等检查确诊肝癌不可切除后,接受TACE术,1周后静脉滴注替雷利珠单抗,每4周重复。3次重复后,磁共振检查提示肿瘤坏死、体积缩小,并能够完全切除。术后病理学检查发现肿瘤完全坏死,肿瘤边缘有大量淋巴细胞浸润,肿瘤周围肝脏组织内广泛存在炎症性肉芽肿,提示TACE联合替雷利珠单抗方案具有杀伤肿瘤和激活机体免疫反应的协同作用。患者术后规范随访,目前已无瘤存活10个月。结论 TACE联合替雷利珠单抗可能是转化治疗不可切除肝癌的有效策略,但需要随机临床试验数据来评估其安全性和有效性。