内皮素对黑素瘤A375细胞转化生长因子-β1及磷酸化Smad 3蛋白表达的调节

Objective To investigate the effects ofendothelin-1 (ET-1) and endothelin-3 (ET-3) on the expression of transformation growth factor-beta 1 (TGF-β1) and phosphorylation of Smad 3 in malignant melanoma cell line, A375. Methods Cultured A375 cells were classified into 5 groups, i.e. control group (no stimulation), ET-1 group (stimulated with ET-1), ET-1+BQ123 group (treated with ET-1 and BQ123),ET-1 + BQ788 group (treated with ET-1 and BQ788), ET-3 group (stimulated with ET-3), to receive different stimulation. The working concentrations were 0, 0.1, 1, 10, 100 nmol/L for ET-1 and ET-3, 10 μmol/L for BQ123 and BQ788. After another 12- and 24-hour culture, ELISA, RT-PCR and Western blot were used to detect the expression of TGF-β1 protein and mRNA as well as phosphorylated Smad 3 (P-Smad 3). Results The expression of TGF-β1 in A375 cells was up-regulated by ET-1, but down-regulated by ET-3, and both of the effects were in a concentration-dependent manner. Under the stimulation with ET-1 and ET-3 of 100 nmol/L, the level of TGF-β1 reached 1289.38 ± 89.42 ng/L per 105 cells and 85.09 ± 9.37 ng/L per 105 cells, respectively, significantly different from that in unstimulated cells (both P < 0.05). BQ123 signifi-cantly blocked the up-regnlatory effect of ET-1 on the expression TGF-β1 protein(P < 0.05), but BQ788 had no significant influence on the effect, so was the case with TGF-β1 mRNA. Western blot revealed that ET-1significantly elevated the expression of P-Smad 3 in A375 cells (P <0.05), and the elevation was significantly inhibited by BQ123, but not by BQ788. The expression of P-Smad 3 was statistically decreased by ET-3 in A375 cells (P <0.05). Conclusions The expression of TGF-β1 could be enhanced by ET-1, but suppressed by ET-3. It is likely that endothelin receptor A mediates the phosphorylation of Smad 3 induced by ET-1.     

《中西医结合临床皮肤性病学》邮购信息

血管内皮生长因子（VEGF）有两种特异性受体,分别为含激酶插入区受体（KDR）和fms样酪氨酸激酶-1（Flt-1）,主要分布在血管法检测VEGF、Flt-1、KDR的mRNA在尖锐湿疣组织的表达,探讨在尖锐湿疣发病机制内皮细胞表面[1].研究表明在人类实体肿瘤中VEGF表达升高[2].本实验用RT-PCR方的作用.……     

男性非淋病性尿道炎患者支原体的检测和对10种抗菌药物的敏感性试验

为了解解脲支原体和人型支原体在男性非淋病性尿道炎(NGU)中的致病作用和它们的体外药物敏感状况,运用微量肉汤稀释法对支原体进行10种抗菌药物的敏感性测定。在268例疑为NGU的男性患者中,85例（31.7%）支原体培养阳性,其中UU感染71例（26.5%）,MH感染4例（1.5%）,MH+UU感染10例（3.7%）,85例阳性患者的支原体株对10种抗菌药物的高度敏感性依次为交沙霉素（81.2%）、克拉霉素（73.0％）、司巴沙星（70.6%）、罗红霉素（67.1%）、阿奇霉素（55.3%）、强力霉素（28.2%）、美满霉素（16.5%）、氧氟沙星（15.3%）、环丙沙星（14.2%）、培氟沙星,发现11株对四环素类和喹诺酮类（除司巴沙星外）交叉耐药。认为支原体的定期耐药性监测,对临床用药有重要意义。     

Olmsted综合征1例及文献复习

报道1例Olmsted综合征,并对国内外文献进行复习,对42例进行总结和分析其临床特点。本例患儿出生后双手足指趾甲呈淡白色,从出生后100d双手足、肛周发生境界清楚的角化性斑块,进行性加重,双手足损害影响患儿行走和抓物,口周也发生角化性红斑,伴有甲营养不良和头发稀疏。根据皮损的形态特点和分布诊断Olmsted综合征。     

误诊为脐茸的脐尿管瘘一例

<正>临床资料患儿,男,5个月。足月顺产,母乳喂养,自脐带脱落后即发现脐部有鲜红色花生大团块,质地柔软,表面无渗液,周围无红肿,局部无触痛,无腹痛、发热等全身不适,我科门诊初步诊断为"脐茸",建议患者至小儿外科就诊后拟诊"脐茸"入院。体格检查:T 36.6℃,P 121次/分,R 28次/分,血压未测,心肺听诊未及明显异常,腹软,未及压痛、反跳痛。皮肤科检查:脐部一鲜红色花生大分叶状肿块,周围无溢液、渗出(图1)。实验室及辅助检查:血、尿常规、     

阴茎鳞状细胞癌1例

1.临床资料 患者男,65岁.阴茎体部乳头样肿块3年.3年前因脑血栓形成合并肺部感染在当地医院治疗,系统应用抗生素(头孢类和喹诺酮类)及对症支持治疗.数天后阴茎体部出现一钱币大红斑,后破溃,伴痒痛不适,皮肤科医师会诊考虑因用药复杂,但抗生素可能性大,系固定型药疹,嘱停用抗生素,外用紫药水,预防感染.出院后阴茎体部破溃面积逐渐扩大,表面呈乳头样突起,偶有少量分泌物.外院以"尖锐湿疣"多次予高频电灼治疗,同时干扰素皮损内注射,无效,且皮损上出现菜花样或结节样外观.患者否认非婚性生活史.系统检查未见明显异常,双侧腹股沟未扪及肿大淋巴结.     

The nongenomic effects of progesterone in repressing iNOS activation through P38MAPK pathways in gonococci-infected polymorphonuclear leukocytes and the clinical significance

Progesterone has nongenomic effects on inducible nitric oxide synthase(iNOS),which is mediated by mitogen activated protein kinase(MAPK)pathways.This effect is supposed to have some potential association with asymptomatic gonococcal infections in women by immunological depression.In this study,polymorphonuclear leukocytes(PMNs)challenged by gonococci were used to study the nongenomic effects of progesterone.The activation of iNOS was assessed by measuring [3H] L-arginine converses to [3H] L-citrulline,and t...     

浅表型基底细胞上皮瘤一例

患者女,84岁,因左上臂内侧局部糜烂迁延不愈6年,于2008年5月17日来我科就诊.患者于6年前无明显诱因出现左上臂内侧局部浅表性糜烂,迁延不愈,皮损缓慢扩大,无明显自觉症状.曾在当地行相关诊治(具体不详),疗效欠佳.     

热休克蛋白90在系统性红斑狼疮外周血单个核细胞中的表达

目的探讨热休克蛋白90(HSP90)在系统性红斑狼疮(SLE)发病中的作用。方法使用免疫组化SP法和细胞原位杂交技术检测45例SLE患者外周血单个核细胞(PBMC)中HSP90蛋白、HSP90mRNA的表达情况,另取15例健康血样进行正常对照。结果45例SLE患者PBMC中HSP90蛋白阳性表达17例(37.78%),15例正常对照组中未见明显阳性细胞。半定量分析SLE活动组PBMC中HSP90蛋白表达的平均吸光度(A)值为(0.412±0.230),显著高于正常对照组(0.117±0.033)和SLE非活动组(0.187±0.019)(均P<0.01);SLE非活动组也显著高于正常对照组(P<0.05)。45例SLE患者中HSP90mRNA阳性表达20例(44.44%),15例正常对照组中未见阳性细胞。半定量分析SLE活动组PBMC中HSP90mRNA表达的平均A值为(0.350±0.225),显著高于正常对照组(0.114±0.009,P<0.01)和SLE非活动组(0.221±0.040,P<0.05);SLE非活动组与正常对照组比较也显著升高(P<0.05)。SLE中抗心磷脂抗体阳性患者HSP90水平明显高于阴性患者。结论SLE患者中存在HSP90的过度表达,且在抗心磷脂抗体阳性的患者中尤其明显,并与疾病的活动性相关。提示HSP90可能在SLE的发病机制中起着重要作用。     

蕈样肉芽肿患者皮损中朗格汉斯细胞的迁移研究

目的：探讨蕈样肉芽肿（MF）患者皮损中皮肤淋巴细胞相关抗原（CLA）、CD62L、共刺激分子（CD40、CD80、CD86）分布及其在树突状细胞（DC）上的表达。方法：将MF皮损作冰冻切片,行单克隆抗体免疫组化染色。结果：肿瘤组织真皮及表皮内可见大量CLA、CD62L、主要组织相容性复合体（MHC）Ⅱ、共刺激分子表达,CLA的表达细胞明显多于CD62L,由斑块期到肿瘤期,表皮内CLA表达数目明显减少,真皮中CLA、CD62L表达增强。CD83^＋DC能表达强的共刺激分子,在基底层、真皮和血管内可见CD83^＋DC表达CD62L,但未成熟DC常表达CLA。结论：MF皮损中朗格汉斯细胞发生迁移,参与了抗肿瘤免疫反应。